Results 121 to 130 of about 2,912,653 (308)
Humanized GPRC6A KGKY is a gain-of-function polymorphism in mice
Scientific Reports, 2020 GPRC6A is proposed to regulate energy metabolism in mice, but in humans a KGKY polymorphism in the third intracellular loop (ICL3) is proposed to result in intracellular retention and loss-of-function.
Min Pi +7 more
doaj +1 more source
Advanced Science, EarlyView.This work designs manganese metal‐organic frameworks (Mn‐MOFs) co‐assembled with Cas12a/crRNA, enabling rapid release of Mn2⁺ under mild conditions. The MOFs‐induced proximity effect sustains Mn2⁺‐Cas12a/crRNA interactions, enhancing the trans‐cleavage activity of complex available to develop an electrochemical biosensor based on Mn‐MOFs‐enhanced ...
Shuai Wu +8 more
wiley +1 more source
Small, Volume 19, Issue 2, January 11, 2023., 2023 For therapeutic genome editing, efficient and cell‐specific transport of the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR‐associated protein 9 (Cas9) system into target cells is desirable. Here, folic acid‐modified Cas9 ribonucleoprotein nanocarriers are presented, which mediate enhanced gene modification in folate receptor
Yi Lin +8 more
wiley +1 more source
CRF: detection of CRISPR arrays using random forest [PDF]
PeerJ, 2017 CRISPRs (clustered regularly interspaced short palindromic repeats) are particular repeat sequences found in wide range of bacteria and archaea genomes. Several tools are available for detecting CRISPR arrays in the genomes of both domains.
Kai Wang, Chun Liang
doaj +2 more sources
Correlation Between GC-content and Palindromes in Randomly Generated Sequences and Viral Genomes [PDF]
arXiv, 2013 GC-content, the ratio of guanine and cytosine bases in an entire nucleotide sequence, and palindromic sequences are unique for every organism due to genomic evolution. The goals of our research was to establish a correlation between GC-content and palindromic densities in wild-type viral and randomly-generated genomes.
arxiv
Advanced Science, EarlyView.Facet joint osteoarthritis (FJOA), prevalent in the elderly, involves Talin2 upregulation promoting ECM degradation while suppressing synthesis. Mechanical load stabilizes Talin2 via OTUB1‐mediated non‐canonical deubiquitination, driving CCL2 expression that recruits immune cells, exacerbating inflammatory joint degeneration.
Yizhen Huang +11 more
wiley +1 more source
CRISPR/Cas9 nuclease cleavage combined with Gibson assembly for seamless cloning
BioTechniques, 2015 Restriction enzymes have two major limitations for cloning: they cannot cleave at any desired location in a DNA sequence and may not cleave uniquely within a DNA sequence. In contrast, the clustered regularly interspaced short palindromic repeat (CRISPR)–
Jia-Wang Wang +6 more
doaj +1 more source
AMB Express, 2021 Salmonella, as a zoonotic pathogen, has attracted widespread attention worldwide, especially in the transmission between household pets and humans. Therefore, we investigated the epidemic distribution of dog Salmonella from pet hospitals and breeding ...
Cheng Yang +5 more
doaj +1 more source
Palindromic length complexity and a generalization of Thue-Morse sequences [PDF]
arXiv, 2019 The notion of palindromic length of a finite word, as well as an infinite word, was first introduced by Frid, Puzynina and Zamboni\cite{FRID2013737}. They conjectured that if the palindromic length of an infinite word is bounded, then this sequence is eventually periodic.
arxiv
Advanced Science, EarlyView.This review explores how multi‐omics, visualization technologies, and AI drive CAR‐T therapy from the micro to the macro level, from preclinical studies to clinical applications. The convergence of these approaches provides deeper insight into CAR‐T cell mechanisms, aids in structural optimization, and significantly enhances treatment efficacy and ...
Yuting Gong +4 more
wiley +1 more source