Results 181 to 190 of about 77,661 (325)

Understanding of endo/lysosomal escape of nanomaterials in biomedical application

Smart Molecules, EarlyView.
This review summarized mainstream mechanisms of endo/lysosomal escape and design strategies of endo/lysosomal escapable nanoparticles in view of surface charge, polarity & hydrophobicity, particle morphology, and lysosomal escape agents. The utilization of endo/lysosomal escapable nanoparticles for vaccine delivery and genetic engineering are ...
Xin Wang, Haoyu Li, Chen Chen, Zhihui Liang   +3 more
wiley   +1 more source

Lipid Nanoparticles for Delivery of CRISPR Gene Editing Components

Small Methods, EarlyView.
The review presents a comprehensive overview of each component of lipid nanoparticles（LNPs）and their effects on editing efficiency. It specifically highlights strategies for achieving non‐liver delivery, aiming for broader applications in gene editing. Furthermore, this review summarizes the applications of LNPs in gene editing and offers insights for ...
Fan Wu, Nei Li, Yudian Xiao, Rohan Palanki, Hannah Yamagata, Michael J. Mitchell, Xuexiang Han   +6 more
wiley   +1 more source

Engineering Pt Single‐Atom Doped SeS2/Ti3CNTx MXene with Molecularly Imprinted Polymer for Precision Pancreatic Cancer Diagnostics: DFT and Molecular Dynamics Perspectives

Small Methods, EarlyView.
A novel approach is developed to pancreatic cancer screening by targeting the cyclophili‐B (CypB)protein using a molecularly imprinted polymer with the electrode material containing Pt single‐atom‐doped/SeS2‐Ti3CNTx MXene. The quantification of CypB in pancreatic cancer tissues provide an insight into the association of CypB with pancreatic cancer ...
Sathish Panneer Selvam, Shanmugasundaram Kamalakannan, Agalya Mathivanan, Sungbo Cho   +3 more
wiley   +1 more source

A densely sampled nuclear phylogenomic analysis of the coryphoid palms (Arecaceae: Coryphoideae)

TAXON, EarlyView.
Abstract The palm family (Arecaceae) has a rich history of phylogenetic research, including several recent phylogenomic studies. However, densely sampled phylogenomic datasets for larger palm clades – such as subfamilies – are still few in number. We used target sequence capture to obtain data for 971 nuclear genes across 421 (ca.
Oscar Wrisberg, Peter Petoe, Paola de Lima Ferreira, Christine D. Bacon, Anders S. Barfod, Sidonie Bellot, Ángela Cano, Thomas L.P. Couvreur, John Dransfield, Andrew Henderson, Fred W. Stauffer, William J. Baker, Wolf L. Eiserhardt   +12 more
wiley   +1 more source

CRISPR/Cas9‐edited tumor‐associated immune cells in cancer immunotherapy

VIEW, EarlyView.
Abstract Immuno‐oncology represents an emerging field that has significantly transformed tumor therapeutics, with immune cells serving as the cellular foundations of cancer immunotherapy. Due to its high efficiency and sensitivity, CRISPR/Cas9 genome editing is a highly promising technique for precise and rapid gene modification.
Yuhui Ma, Junxin Chen, Yuan Du, Zhihao Cao, Weizhi Ji, Yuyu Niu, Zebin Yang   +6 more
wiley   +1 more source

A palindromic RNA sequence as a common breakpoint contributor to copy-choice recombination in SARS-COV-2

, 2020
William R. Gallaher
openalex   +1 more source

Role of mitochondrial complex I genes in host plant expansion of Bactrocera tau (Tephritidae: Diptera) by CRISPR/Cas9 system

Insect Science, EarlyView.
The role of 3 mitochondrial complex I gene in host expansion of Bactrocera tau was verified by CRISPR/Cas9 gene editing system. A stable homogenous strain (Btndufa7‐/‐) of B.tau was established after silencing Ndufa7. The reduced size of larvae and pupae of Ndufa7 knockout strain was first found.Then different levels of fitness and relative expression ...
Wei Shi, Linsheng He, Ruixiang Li, Jun Cao   +3 more
wiley   +1 more source

The transformer gene controls sexual development in Drosophila suzukii

Insect Science, EarlyView.
The transformer gene plays a key role in the genetic pathway for sexual development in Drosophila suzukii Abstract The genetic network of sex determination in the model organism Drosophila melanogaster was investigated in great detail. Such knowledge not only advances our understanding of the evolution and regulation of sexual dimorphism in insects ...
Ying Yan, Jing Zhao, Jonas Schwirz, Cristina Borghesi, Conghui Liu, Bo Liu, Wanqiang Qian, Fanghao Wan, Marc F. Schetelig   +8 more
wiley   +1 more source

Detection of mutations induced by CRISPR/Cas9 in cabbage protoplasts using qPCR and dPCR

Annals of Applied Biology, EarlyView.
This study evaluated two PCR‐based methods—qPCR and dPCR—for detecting CRISPR‐induced mutations in transformed cabbage protoplasts. Both methods distinguished edited from wild‐type protoplasts, offering rapid and cost‐efficient alternatives for mutation detection, with dPCR providing higher sensitivity and closer agreement with NGS results.
Ester Stajič, Žiga Javornik, Nataša Štajner   +2 more
wiley   +1 more source

Causal Relationships Between Gastroesophageal Reflux Disease and Myocardial Infarction: Insights From Univariable and Multivariable Mendelian Randomization Analyses

Annals of Human Genetics, EarlyView.
ABSTRACT Background Observational studies have indicated that gastroesophageal reflux disease (GERD) is connected to myocardial infarction (MI). Nonetheless, the question of causality in these relationships remains unresolved, given the potential influence of confounding variables.
Xiaoya Zheng, Teng Hu, Tianxiang Fang, Pengpeng Su, Yingsong Wang, Ning Huangfu   +5 more
wiley   +1 more source