Results 261 to 270 of about 2,912,653 (308)
Cas9‐Rep fusion tethers donor DNA in vivo and boosts the efficiency of HDR‐mediated genome editing
Summary Genome editing based on the homology‐directed repair (HDR) pathway enables scar‐free and precise genetic manipulations. However, the low frequency of HDR hinders its application in plant genome editing. In this study, we engineered the fusion of Cas9 and a viral replication protein (Rep) as a molecular bridge to tether donor DNA in vivo, which ...
Zhentao Zhou+7 more
wiley +1 more source
Palindromic but not G-rich sequences are targets of class switch recombination
Junko Tashiro+2 more
openalex +1 more source
9311 allele of OsNAR2.2 enhances nitrate transport to improve rice yield and nitrogen use efficiency
Summary Improving nitrogen use efficiency (NUE) in rice is a requirement for future sustainable agricultural production. However, key factors and regulatory networks involved in NUE remain unclear. Here, QTL analysis, fine‐mapping and functional validation demonstrated that qCR4 encodes a putative high‐affinity nitrate transporter‐activating protein 2 ...
Linlin Hou+17 more
wiley +1 more source
Summary Pomegranate is an important perennial fruit tree distributed worldwide. Reference genomes with gaps and limit gene identification controlling important agronomic traits hinder its functional genomics and genetic improvements. Here, we reported a telomere‐to‐telomere (T2T) gap‐free genome assembly of the distinctive cultivar ‘Moshiliu’.
Lina Chen+18 more
wiley +1 more source
Statistical analysis on avoidance of palindromic sequences in genomes of microorganisms
Tomas Rekašius, Albertas Timinskas
openalex +2 more sources
HB52‐PUT2 Module‐Mediated Polyamine Shoot‐to‐Root Movement Regulates Salt Stress Tolerance in Tomato
ABSTRACT Soil salinity severely restricts crop quality and yields. Plants have developed various strategies to alleviate salinity stress's negative effects, including polyamine redistribution by polyamine uptake transporters (PUTs). However, the mechanisms by which PUTs alter polyamine translocation processes during salt stress have not been fully ...
Xian Yang+7 more
wiley +1 more source
Identification of Target Genes Using Innovative Screening Systems
ABSTRACT Advances in cancer biology have been achieved by the identification of oncogenes and tumor suppressor genes through the remarkable progression of next‐generation sequencing. New techniques, such as single‐cell analysis, help uncover cancer progression and heterogeneity.
Keisuke Sugita, Morito Kurata
wiley +1 more source
Non‐conserved microRNAs and their roles in plants: the case for legumes
Non‐conserved miRNAs are functional and modulate stress and microbial interactions in legumes, hinting at broader roles in plant gene regulation. Abstract Several classes of small RNAs function to regulate stress and development pathways in all kingdoms of life.
Y. Hernández+3 more
wiley +1 more source