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Elastase Activity in Human Pancreatic Secretions
Nature, 1962IN 1949, Balo and Banga1 first reported the isolation of the pancreatic enzyme elastase, which was unique in its ability to render soluble elastin (an important structural component of arteries, veins, skin and lung). This enzyme was first believed to be mucolytic in action, but it is now established that it is also a potent proteolytic agent2.
S, TINDEL +3 more
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ELISA for human pancreatic elastase 1
Clinical Biochemistry, 1989A sandwich-type ELISA for human pancreatic elastase 1 was developed and assessed for clinical feasibility. Using highly purified reagents, the test seems to be as sensitive and specific as the well-documented Radio-immunoassay (RIA) for the detection of elevated elastase 1 serum levels and, consequently, would have the same indications described for ...
A, Sziegoleit, H, Knäpler, B, Peters
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Mechanism of elastolysis by pancreatic elastase
Experientia, 1957Nous presentons une etude du mecanisme de l'elastolyse par une elastase partiellement purifiee du pancreas. La methode utilisee consiste dans le dosage spectrophotometrique de l'«azoelastine» dissoute. Cette proteine n'est que tres lentement hydrolysee par la trypsine et chymotrypsine cristallisees.
L, ROBERT, P, SAMUEL
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Urinary Elastase 1 in Chronic Pancreatic Disease
Enzyme, 1989Serum and urine elastase 1, its renal output and clearance and urinary gamma-glutamyltransferase and ribonuclease excretions were measured in 16 patients with pancreatic cancer, 23 with chronic pancreatitis and in 22 healthy controls in order to evaluate elastase 1 plasma-urine transfer in chronic pancreatic disease and to investigate any factors that ...
C. Fabris +12 more
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Serum human pancreatic elastase I levels in pancreatic disease
Clinica Chimica Acta, 1987The serum elastase concentration has been measured in 25 healthy individuals, sixteen patients with tumours involving the pancreas, nine patients with acute pancreatitis and nine patients with chronic pancreatitis. The elastase concentrations were increased in those with pancreatic tumours particularly when the head of the pancreas was involved.
P K, Buamah +3 more
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Digestion of elastin by porcine pancreatic elastase I and elastase II
International Journal of Peptide and Protein Research, 1985Elastin was fully solubilized by digestion with elastase I or elastase II. Each digest was separated into high‐molecular weight and low‐molecular weight fractions that were characterized by the correspondence to their amino acid content, N‐terminal sequence and C‐terminal amino acids. It was found that although the relative amount of amino acids in the
M, Vered, Y, Burstein, A, Gertler
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1971
Publisher Summary This chapter focuses on porcine pancreatic elastase. The ability of elastase to degrade elastin forms the basis for many assay methods that involve the measurement of the amount of insoluble elastin solubilized by elastase digestion in a certain time.
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Publisher Summary This chapter focuses on porcine pancreatic elastase. The ability of elastase to degrade elastin forms the basis for many assay methods that involve the measurement of the amount of insoluble elastin solubilized by elastase digestion in a certain time.
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1962
Publisher Summary As the mechanism of action of elastase is not definitely known, all assay methods are based on measuring the amount of insoluble substrate solubilized in a certain time. The assay described can be used with crude preparations, as other pancreatic enzymes do not attack elastin.
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Publisher Summary As the mechanism of action of elastase is not definitely known, all assay methods are based on measuring the amount of insoluble substrate solubilized in a certain time. The assay described can be used with crude preparations, as other pancreatic enzymes do not attack elastin.
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Substrate specificity of human pancreatic elastase 2
Biochemistry, 1980The substrate specificity of human pancreatic elastase 2 was investigated by using a series of peptide p-nitroanilides. The kinetic constants, kcat and Km, for the hydrolysis of these peptides revealed that this serine protease preferentially hydrolyzes peptides containing P1 amino acids which have medium to large hydrophobic side chains, except for ...
E G, Del Mar +4 more
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Serum elastase 1 in chronic pancreatic disease
Klinische Wochenschrift, 1985Elastase 1 and immunoreactive trypsin were assessed by a RIA technique in the sera of 29 control subjects, 24 pancreatic cancer patients, 22 patients with chronic pancreatitis and 31 with extra-pancreatic diseases to ascertain and compare their usefulness in chronic pancreatic disease diagnosis.
DEL FAVERO G. +7 more
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