Results 91 to 100 of about 108,019 (305)

Colorectal Cancer and Lactobacillus plantarum: A qPCR-Based Study of FFPE Tissues [PDF]

Journal of Basic Research in Medical Sciences
Introduction: Colorectal cancer (CRC) is a major global health issue. The gut microbiota has become an important factor in CRC development, with increased focus on microbes such as Lactobacillus plantarum.
Farzaneh Korani, NayebAli Rezvani, Behrooz Sadeghi kalani, Reza Pakzad, Parisa Asadollahi   +4 more
doaj  

Protocol for preparing formalin-fixed paraffin-embedded musculoskeletal tissue samples from mice for spatial transcriptomics

STAR Protocols
Summary: Here, we present a protocol for using spatial transcriptomics in bone and multi-tissue musculoskeletal formalin-fixed paraffin-embedded (FFPE) samples from mice.
Esther Wehrle, Denise Günther, Neashan Mathavan, Amit Singh, Ralph Müller   +4 more
doaj   +1 more source

Protocol for tissue processing and paraffin embedding of mouse brains following ex vivo MRI. [PDF]

STAR Protoc, 2023
Smart A, Tisca C, Huszar IN, Kor D, Ansorge O, Tachrount M, Smart S, Lerch JP, Miller KL, Martins-Bach AB.   +9 more
europepmc   +1 more source

Dual PI3K/AKT and CDK4/6 inhibition reveals selective sensitivity in an SHH medulloblastoma stem cell model

Molecular Oncology, EarlyView.
Targeted therapy was evaluated in SHH medulloblastoma using neuroepithelial stem cell (NES) and tumor‐derived NES‐like (tNES) models in 2D monolayers and 3D spheroids. PI3K, AKT, and CDK4/6 inhibitors had minimal effects in NES but markedly reduced viability and growth and induced apoptosis in tNES cells, revealing distinct therapeutic vulnerabilities.
Monika Lukoseviciute, Madeleine Birgersson, Paolo Ceriani, Margareta Wilhelm, Ourania N Kostopoulou   +4 more
wiley   +1 more source

Continuous Separation of Light Olefin/Paraffin Mixtures on ZIF-4 by Pressure Swing Adsorption and Membrane Permeation

Molecules, 2018
In this study, two zeolitic imidazolate frameworks (ZIFs) called ZIF-4 and ZIF-zni (zni is the network topology) were characterized by sorption studies regarding their paraffin/olefin separation potential.
Maximilian Hovestadt, Sebastian Friebe, Lailah Helmich, Marcus Lange, Jens Möllmer, Roger Gläser, Alexander Mundstock, Martin Hartmann   +7 more
doaj   +1 more source

Establishment of a humanized patient‐derived xenograft mouse model of high‐grade serous ovarian cancer for preclinical evaluation of combination immunotherapy

Molecular Oncology, EarlyView.
We have established a humanized orthotopic patient‐derived xenograft (Hu‐oPDX) mouse model of high‐grade serous ovarian cancer (HGSOC) that recapitulates human tumor–immune interactions. Using combined anti‐PD‐L1/anti‐CD73 immunotherapy, we demonstrate the model's improved biological relevance and enhanced translational value for preclinical ...
Luka Tandaric, Line Bjørge, Martine Rott Lode, Cecilie Fredvik Torkildsen, Pia Aehnlich, Rammah Elnour, Daniela Elena Costea, Lars Andreas Akslen, Liv Cecilie Vestrheim Thomsen, Emmet McCormack, Katrin Kleinmanns   +10 more
wiley   +1 more source

Formalin fixation and paraffin embedding interfere with the preservation of optical metabolic assessments based on endogenous NAD(P)H and FAD two-photon excited fluorescence. [PDF]

Biomed Opt Express, 2023
Sánchez-Hernández A, Polleys CM, Georgakoudi I.   +2 more
europepmc   +1 more source

Deciphering transcriptional plasticity in pancreatic ductal adenocarcinoma reveals alterations in sensory neuron innervation

Molecular Oncology, EarlyView.
Pancreatic sensory neurons innervating healthy and PDAC tissue were retrogradely labeled and profiled by single‐cell RNA sequencing. Tumor‐associated innervation showed a dominant neurofilament‐positive subtype, altered mitochondrial gene signatures, and reduced non‐peptidergic neurons.
Elena Genova, Michele Montrone, Uday Rangaswamy, Francesco Diversi, Irene Schiavo, Denise Ferrarini, Roberta Di Florio, Irene Longo, Michele Coscia, Nicola Zamboni, Giorgia Demontis, Lisa Veghini, Vincenzo Corbo, Remo Sanges, Paul Heppenstall   +14 more
wiley   +1 more source

Anatomical Structure of Young Oil Palm (Elaeis guineensis Jacq.) Leaves for Callogenesis Initiation

Biology, Medicine & Natural Product Chemistry
Plant anatomy, a key area within biology, examines the structure and organization of plant organs and tissues. It underpins various disciplines such as physiology, ecology, taxonomy, and evolutionary biology.
Muhammad Ilham, Ernayunita Ernayunita
doaj   +1 more source

Enzyme-labeled Antigen Method: Factors Influencing the Deterioration of Antigen-binding Activity of Specific Antibodies during Formalin Fixation and Paraffin Embedding. [PDF]

Acta Histochem Cytochem, 2022
Mizutani Y, Shiogama K, Inada KI, Takeuchi T, Niimi A, Suzuki M, Tsutsumi Y.   +6 more
europepmc   +1 more source