Ex Vivo and In Vitro Proteomic Approach to Elucidate the Relevance of IL-4 and IL-10 in Intervertebral Disc Pathophysiology. [PDF]
Bermudez-Lekerika P +9 more
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A pediatric case of membranous-like glomerulopathy with masked IgG kappa deposits. [PDF]
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Histological Analysis of the Prostate During the Human Fetal Period (12 to 22 Weeks Post-Conception). [PDF]
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Epigenetic similarity between adenoid ameloblastoma and dentinogenic ghost cell tumour: DNA methylation analysis in odontogenic tumours. [PDF]
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Softening Paraffin-Embedded Plant Tissues
Stain Technology, 1953Soaking paraffin-embedded plant specimens 2–3 days at 37°C. in a mixture of glycerol, 10 ml., Dreft, 1 g., and water 90 ml. is an effective means of softening them prior to sectioning. One side of the paraffin block must be pared away to expose the tissue before immersion in the softening solution.
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Decalcifying Tissues for Paraffin Embedding
Cold Spring Harbor Protocols, 2008INTRODUCTIONParaffin sections of bone usually require a decalcification step after fixation before sectioning. This protocol describes a method for decalcifying fixed tissue.
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Chemical Dehydration for Rapid Paraffin Embedding
Biotechnic & Histochemistry, 1994We describe chemical dehydration with 2,2-dimethoxypropane (DMP) for rapid paraffin embedding using a mixture of DMP and mineral oil followed by mineral oil as clearing intermediates. This method is useful for classical histological techniques as well as for histochemistry and immunocytochemistry.
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Cutting Sections of Paraffin-Embedded Tissues
Cold Spring Harbor Protocols, 2008INTRODUCTIONThis protocol describes the sectioning of tissues embedded in paraffin blocks. Paraffin sections require extensive fixation and processing steps but provide superior morphology compared with other sectioning methods. Sectioning paraffin blocks requires experience and should be learned from an experienced researcher, if possible.
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