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DNA Extraction from Paraffin-Embedded Tissues
2003In routine histopathology, most tissues are fixed in formalin and embedded in paraffin for long-term preservation. DNA can be extracted from these tissues for subsequent molecular analysis by amplification methods. We describe herein a protocol for DNA preparation from paraffin-embedded tissues based on published procedures (1-3).
H, Fan, M L, Gulley
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Paraffin Embedding Tissue Samples for Sectioning
Cold Spring Harbor Protocols, 2008INTRODUCTIONThis protocol describes a method for embedding tissues in paraffin blocks for sectioning. Paraffin sections require extensive fixation and processing steps, but provide superior morphology compared with other sectioning methods.
Fischer, Andrew H. +3 more
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Multiparameter Immunofluorescence on Paraffin-Embedded Tissue Sections
Applied Immunohistochemistry & Molecular Morphology, 2006Immunohistochemical techniques have gained increasing importance in diagnostics and research. While formalin-fixed, paraffin-embedded human tissue retains excellent morphology, the detection of antigens by immunofluorescence in its sections and especially the demonstration of multiple simultaneous antibodies have limitations.
Frauke, Bataille +7 more
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Making Formalin-Fixed, Paraffin Embedded Blocks
2018Paraffin embedding is a standard technique used in clinical and research laboratories to create a formalin-fixed, paraffin-embedded (FFPE) block of tissue. Formalin-fixed tissue undergoes tissue processing and then is embedded in paraffin (wax) to create a FFPE block or paraffin block.
Alireza, Sadeghipour +1 more
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Staining Paraffin Embedded Sections of Scald of Barley before Paraffin Removal
Biotechnic & Histochemistry, 1997Staining of paraffin embedded sections with periodic acid-Schiff reagent and fast green before paraffin removal resulted in differentiation of barley seed and leaf tissue from fungal structures of Rhynchosporium secalis. Crystal violet, toluidine blue O and antiline blue also successfully stained fungal structures of R.
K, Xi, P A, Burnett
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Improvement of the Butyl Methacrylate-Paraffin Embedment
Stain Technology, 1983The excellent butyl methacrylate-paraffin method as an embedment for light microscopy has been technically improved. More uniform and reproducible polymerization has been obtained by using a vacuum oven to degas the polymerizing mixture and to replace the air with nitrogen at 650 Torr. The amount of benzoyl peroxide required must be determined for each
P J, McMillan +3 more
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A Sample-Grouping Technique for Paraffin Embedments
Stain Technology, 1983A technique is described which facilitates histological preparation of multiple tissue specimens for light microscopy. The procedure enables the investigator to separate and label identifiable subgroups from a larger number of specimens in one histological section. After standard fixation, murine esophagi were arranged longitudinally and secured within
N H, Rubin, P S, Baur
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2007
1. Nakon prethodne fiksacije i dehidracije tkivo se obično nalazi u tzv. intermediju. Najčešće se u tu svrhu koristi ksilol. Tkivo se mora prožeti intermedijem sve dok ne padne na dno posude odnosno ne postane prozirno3. 2. Uzorak tkiva prebaciti u posudicu s mješavinom ksilola i parafina (1:1) (termostat, 56° C 10 min.)4. 3. Tkivo staviti u posudicu s
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1. Nakon prethodne fiksacije i dehidracije tkivo se obično nalazi u tzv. intermediju. Najčešće se u tu svrhu koristi ksilol. Tkivo se mora prožeti intermedijem sve dok ne padne na dno posude odnosno ne postane prozirno3. 2. Uzorak tkiva prebaciti u posudicu s mješavinom ksilola i parafina (1:1) (termostat, 56° C 10 min.)4. 3. Tkivo staviti u posudicu s
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Interphase Cytogenetics in Paraffin-Embedded Ovarian Tissue
2003Interphase cytogenetics using formalin-fixed/paraffin-embedded tissue is now a well-established technique, which renders it possible to obtain "cytogenetic information" from interphase nuclei of solid tumors (1,2, for ovarian cancer, e.g., 3-8). It is the only tool to investigate specific numerical chromosomal aberrations (3-7), amplification.
S, Neubauer, T, Liehr
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Embedding Paraffins for Eye Microtomy
Journal of Histotechnology, 1977Abstract Embedding paraffins commonly used for eye microtomy are discussed. A relatively new paraffin product may be used also for eye microtomy and light microscopy preparations of excellent quality.
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