An optimized histological proceeding to study the female gametophyte development in grapevine
Plant Methods, 2020 Background Reproductive success in seed plants depends on a healthy fruit and seed set. Normal seed development in the angiosperms requires the production of functional female gametophytes. This is particularly evident in seedless cultivars where defects
P. Moreno-Sanz +4 more
doaj +1 more source
Diagnostic Pathology, 2021 Background Quick and reliable testing of EGFR and KRAS is needed in non-small cell lung cancer (NSCLC) to ensure optimal decision-making for targeted therapy.
Saskia Offerman +5 more
doaj +1 more source
Lineage dynamics of murine pancreatic development at single-cell resolution. [PDF]
, 2018 Organogenesis requires the complex interactions of multiple cell lineages that coordinate their expansion, differentiation, and maturation over time.
Byrnes, Lauren E +8 more
core +2 more sources
The Paraffin-Embedded RNA Metric (PERM) for RNA Isolated from Formalin-Fixed, Paraffin-Embedded Tissue [PDF]
BioTechniques, 2016 RNA isolated from formalin-fixed, paraffin-embedded (FFPE) tissue is commonly evaluated in both investigative and diagnostic pathology. However, the quality of the data is directly impacted by RNA quality. The RNA integrity number (RIN), an algorithm based on a combination of electrophoretic features, is widely applied to RNA isolated from paraffin ...
Joon-Yong Chung +2 more
openaire +4 more sources
Enhanced molecular analyses by combination of the HOPE-technique and laser microdissection
Diagnostic Pathology, 2006 As part of an investigation aimed at illuminating the possibilities and limits of the HOPE-fixation and paraffin-embedding technique we here describe a novel procedure which was developed in order to combine the benefits of the HOPE-technique with the ...
Lang Dagmar +7 more
doaj +1 more source
TmaDB: a repository for tissue microarray data [PDF]
, 2005 Background: Tissue microarray (TMA) technology has been developed to facilitate large, genome-scale molecular pathology studies. This technique provides a high-throughput method for analyzing a large cohort of clinical specimens in a single experiment ...
Quirke, P, Sharma-Oates, A, Westhead, DR
core +3 more sources
Utility of squash smear cytology in fiber-optic bronchoscopic biopsies
Journal of Cytology, 2014 Background: Fiber-optic bronchoscopic biopsies yield very small bits of tissue, leading to high false negativity in lung cancer diagnosis, after paraffin embedding.
Sangeetha Keloth Nayanar +2 more
doaj +1 more source
Human prostate sphere-forming cells represent a subset of basal epithelial cells capable of glandular regeneration in vivo. [PDF]
, 2009 BackgroundProstate stem/progenitor cells function in glandular development and maintenance. They may be targets for tumor initiation, so characterization of these cells may have therapeutic implications.
Garraway, Isla P +11 more
core +1 more source
Proteomic analysis of RCL2 paraffin‐embedded tissues [PDF]
Journal of Cellular and Molecular Medicine, 2008 AbstractHistopathological diagnosis in most of the world's hospitals is based upon formalin‐fixed and paraffin‐embedded (FFPE) tissues. Although this standard fixation and embedding procedure keeps the tissue in excellent form for morphological and immunohistological analysis, FFPE is inappropriate for nucleic acids and protein studies. We investigated
Bellet, V +8 more
openaire +2 more sources
Improved arteriogenesis with simultaneous skeletal muscle repair in ischemic tissue by SCL plus multipotent adult progenitor cell clones from peripheral blood [PDF]
, 2004 Background: The CD34- murine stem cell line RM26 cloned from peripheral blood mononuclear cells has been shown to generate hematopoietic progeny in lethally irradiated animals.
Gottgens, Berthold +7 more
core +1 more source