Results 321 to 330 of about 1,356,440 (380)

Analysis of the vasculature by immunohistochemistry in paraffin-embedded brains

Brain Structure and Function, 2017
The brain vasculature can be investigated in different ways ranging from in vivo to biochemical analysis. Immunohistochemistry is a simple and powerful technique that can also be applied to archival tissues. However, staining of brain vessels on paraffin sections has been challenging.
Yann Decker, Andreas Müller, Eszter Németh, Walter J. Schulz-Schaeffer, Marc Fatar, Michael D. Menger, Yang Liu, Klaus Fassbender   +7 more
openaire   +3 more sources

Multiparameter Immunofluorescence on Paraffin-Embedded Tissue Sections

Applied Immunohistochemistry & Molecular Morphology, 2006
Immunohistochemical techniques have gained increasing importance in diagnostics and research. While formalin-fixed, paraffin-embedded human tissue retains excellent morphology, the detection of antigens by immunofluorescence in its sections and especially the demonstration of multiple simultaneous antibodies have limitations.
Frank Klebl, Benjamin Stoelcker, Gerhard Rogler, Ferdinand Hofstädter, Frauke Bataille, Sabine Troppmann, Petra Rümmele, Anja-Katrin Bosserhoff   +7 more
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Staining Paraffin Embedded Sections of Scald of Barley before Paraffin Removal

Biotechnic & Histochemistry, 1997
Staining of paraffin embedded sections with periodic acid-Schiff reagent and fast green before paraffin removal resulted in differentiation of barley seed and leaf tissue from fungal structures of Rhynchosporium secalis. Crystal violet, toluidine blue O and antiline blue also successfully stained fungal structures of R.
K Xi, P A Burnett
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A Disposable Plastic Box for Paraffin Embedding

Stain Technology, 1958
A sheet of cellulose acetate about 0.01 inch thick is clamped over a mold, heated to softness by an electric heater and drawn down over the mold by means of a vacuum. When cooled, the sheet, now formed into embedding boxes, is removed from the clamp. Boxes so made are inexpensive enough to be disposable but can be reused, since the sides of the boxes ...
openaire   +3 more sources

A review of preanalytical factors affecting molecular, protein, and morphological analysis of formalin-fixed, paraffin-embedded (FFPE) tissue: how well do you know your FFPE specimen?

Archives of Pathology & Laboratory Medicine, 2014
CONTEXT Formalin fixation and paraffin embedding is a timeless, cost-efficient, and widely adopted method of preserving human tissue biospecimens that has resulted in a substantial reservoir of formalin-fixed, paraffin-embedded blocks that represent both
B. Bass, K. B. Engel, S. Greytak, Helen M. Moore   +3 more
semanticscholar   +1 more source

A Method for the Use of Immunofluorescence on Paraffin-embedded Tissues

American Journal of Clinical Pathology, 1978
Immunofluorescence is increasingly used for the visualization of antigens and antibodies in various tissues. The commonly used frozen sections present several disadvantages, which can be avoided by the present adaptation of paraffin-embedded sections for immunofluorescence.
Brent H. Dorsett, Harry L. Ioachim
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PLANT MICROTECHNIQUE: SOME PRINCIPLES AND NEW METHODS

, 1968
Some easily seen structural features of living plant cells are destroyed or badly distorted by most of the common fixatives and embedding media used in plant histology.
N. Feder, T. P. O’brien
semanticscholar   +1 more source

Stain for Myelin Sheaths in Tissues Embedded in Paraffin [PDF]

American Journal of Clinical Pathology, 1937
Although methods for staining myelin sheaths in material embedded in paraffin have been described, 1 there has not been found in the literature to date a technic that does not require differentiation. In working with the modification of the Pal-Weigert stain described by Clark and Ward, 2 it was observed that their method, when applied to sections ...
openaire   +2 more sources

Tissue fixed with formalin and processed without paraffin embedding is suitable for imaging of both peptides and lipids by MALDI‐IMS

Proteomics, 2016
M. Pietrowska, M. Gawin, J. Polańska, P. Widłak   +3 more
semanticscholar   +1 more source

DNA Extraction from Paraffin-Embedded Tissues

2003
In routine histopathology, most tissues are fixed in formalin and embedded in paraffin for long-term preservation. DNA can be extracted from these tissues for subsequent molecular analysis by amplification methods. We describe herein a protocol for DNA preparation from paraffin-embedded tissues based on published procedures (1-3).
Hongxin Fan, Margaret L. Gulley
openaire   +3 more sources