Screening and epitope characterization of Nidogen‐2‐specific nanobodies
FEBS Open Bio, EarlyView.Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen +9 more
wiley +1 more source
PARAMETRIC SYNTHESIS OF MODELS FOR MULTICRITERIAL ESTIMATION OF TECHNOLOGICAL SYSTEMS
Сучасний стан наукових досліджень та технологій в промисловості, 2017 The subject matter of the article is the problem of multicriteria estimation of the properties of technological systems (TS) in the process of their structural-parametric optimization. The goal of the study is to increase the efficiency of procedures for
Vladimir Beskorovainyi
doaj +1 more source
A Non-parametric Approach to Small Area Estimation with Application to Madhya Pradesh, India
, 2021 Aalok Ranjan Chaurasia
openalex +1 more source
Estimation in Single-Index Panel Data Models with Heterogeneous Link Functions [PDF]
In this paper, we study semiparametric estimation for a single-index panel data model where the nonlinear link function varies among the individuals.
Jia Chen, Jiti Gao, Degui Li
core
Tracking-Based Non-Parametric Background-Foreground Classification in a Chromaticity-Gradient Space
, 2010 This work presents a novel background-foreground classification technique based on adaptive non-parametric kernel estimation in a color-gradient space of components.
Narciso Garcia +3 more
core +1 more source
Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system
FEBS Open Bio, EarlyView.An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková +3 more
wiley +1 more source
Computer Assisted Methods in Engineering and Science, 2023 This paper concerns modelling of surfaces resulting from measurements or from digital simulation of surfaces e.g., tooth flanks, based on the theory of gearing, with intentionally introduced modification usually defined in discrete form.
Andrzej Kawalec
doaj
Semi-Parametric Estimation Using Bernstein Polynomial and a Finite Gaussian Mixture Model. [PDF]
Entropy (Basel), 2022 Helali S, Masmoudi A, Slaoui Y.
europepmc +1 more source
Semiparametric Regression Estimation in Null Recurrent Nonlinear Time Series [PDF]
Estimation theory in a nonstationary environment has been very popular in recent years. Existing studies focus on nonstationarity in parametric linear, parametric nonlinear and nonparametric nonlinear models. In this paper, we consider a partially linear
Jia Chen, Jiti Gao, Degui Li
core
Systemic dysregulation of apolipoproteins in amyotrophic lateral sclerosis serum
FEBS Open Bio, EarlyView.Amyotrophic lateral sclerosis (ALS) is a fatal disease that damages motor neurons. This study found that people with ALS show significant changes in blood fats and the proteins that carry them. Several apolipoproteins were higher, lipid balances were altered, and normal protein–lipid relationships were disrupted.
Finula I. Isik +6 more
wiley +1 more source