Results 41 to 50 of about 81,757 (265)
Biophysical approaches for studying viral entry
Viruses infect all living organisms and have been responsible for major epidemics and pandemics. Their ongoing evolutionary battle with host defenses creates a constant need for improved tools to study viral behavior. Advancing methods to probe viral attachment, fusion, and genome release deepen our understanding of how infections begin and support the
Inbar Yosibash, Raya Sorkin
wiley +1 more source
We present robust protocols for the preparation of supported lipid bilayers (SLBs) incorporating either Salmonella smooth LPS or outer membrane vesicles (OMVs). We use a combination of quartz crystal microbalance with dissipation (QCM‐D) and fluorescence microscopy to both characterize the SLBs of various compositions and to probe their interactions ...
Hudson P. Pace +6 more
wiley +1 more source
Peripheral lysosomes recruit PLEKHG3 to focal adhesions and restrain protrusion dynamics
Proximity‐dependent labeling at the LAMTOR complex revealed the Rho GEF PLEKHG3 as a lysosome‐proximal protein directing the study toward the influence of lysosome positioning on actin dynamics and cell motility. We show that PLEKHG3 colocalizes with lysosomes at focal adhesion sites and observe that forced peripheral dispersion of lysosomes hinders ...
Rainer Ettelt +8 more
wiley +1 more source
Abstract The formation of particle tracks, and such phenomena as the detection of charged particles, and the damage produced by charged particles, are intimately related to the spatial distribution of ionization energy deposited by δ-rays. Changes in the spectrum of δ-rays with the velocity of the primary particle, imply that linear measures of the ...
R. Katz, E. J. Kobetich
openaire +3 more sources
Golgi enzymes are retrieved from the plasma membrane to the trans‐Golgi network
Golgi enzymes are traditionally considered resident proteins retained within the Golgi apparatus. Here, we demonstrate that a subset transiently reaches the cell surface and is subsequently retrieved to the trans‐Golgi network via retrograde transport. Using a nanobody‐based toolkit, we uncover a dynamic trafficking cycle of several Golgi enzymes.
Dominik P. Buser, Tina Junne
wiley +1 more source
Deteksi dan Pelacakan Wajah Berdasarkan Warna Kulit Menggunakan Partikel Filter
Face detection and tracking are one of the areas which always in progress in many applications such as security system, biometric and so on. However, face detection and tracking can become a complex problem when it is done in real time condition.
Budi Sugandi
doaj +1 more source
Particle tracking modeling of sediment-laden jets [PDF]
This paper presents a general model to predict the particulate transport and deposition from a sediment-laden horizontal momentum jet. A three-dimensional (3-D) stochastic particle tracking model is developed based on the governing equation of particle ...
S. N. Chan, J. H. W. Lee
doaj +1 more source
BNP-Track: A framework for multi-particle superresolved tracking
When tracking fluorescently labeled molecules (termed "emitters") under widefield microscopes, point spread function overlap of neighboring molecules is inevitable in both dilute and especially crowded environments. In such cases, superresolution methods leveraging rare photophysical events to distinguish static targets nearby in space introduce ...
Lance W.Q. Xu +3 more
openaire +2 more sources
Synergistic perspectives—How single‐molecule biophysics complement biochemical understanding
In this review, we discuss how ensemble biochemistry and single‐molecule approaches are complementary, outline commonly used single‐molecule techniques, and illustrate their relevance through two representative case studies: chromatin organization by SMC complexes and pathway choice during DNA double‐strand break repair.
Sara De Bragança +2 more
wiley +1 more source

