Results 61 to 70 of about 81,757 (265)

Three-dimensional spatiotemporal tracking of fluorine-18 radiolabeled yeast cells via positron emission particle tracking. [PDF]

open access: yesPLoS ONE, 2017
A method for Positron Emission Particle Tracking (PEPT) based on optical feature point identification techniques is demonstrated for use in low activity tracking experiments.
Seth T Langford   +5 more
doaj   +1 more source

Evaluating the involvement of autolysosomes in the nuclear translocation of fluorescent proteins

open access: yesFEBS Open Bio, EarlyView.
Endogenously expressed fluorescent proteins can be degraded by autophagy and transported to cell nuclei via the nuclear pore complex. But in some cell lines, for example, HeLa cells which are positive for immunoreactivity of a receptor ligand, such as UCN I, in cell nuclei, fusion of autolysosome with the nuclear envelope is involved in the nuclear ...
Keiichi Ikeda
wiley   +1 more source

From energy provision to protein synthesis: Tunnelling nanotubes as mediators of intercellular metabolic cooperation in cancer

open access: yesFEBS Open Bio, EarlyView.
The cytoskeleton‐mediated transport of mitochondria via tunnelling nanotubes restores respiration, increases ATP production, rescues cells from apoptosis, activates the AKT/mTOR signalling pathway, promotes cell migration and invasiveness, contributes to cancer progression and treatment resistance.
Stanislava Martínková, Jan Trnka
wiley   +1 more source

On the performance of particle tracking

open access: yesJournal of Fluid Mechanics, 1987
The sources of error associated with the use of particle-tracking techniques in the measurement of velocity and vorticity fields in moderately three-dimensional turbulent flows are analysed. The two dominant sources of error are the visualisation error, resulting from the limited resolution of the optical data acquisition system, and the sampling error,
Agüì, Juan C.   +1 more
openaire   +2 more sources

Accurate and noninvasive prostate cancer detection using plasma‐derived extracellular vesicle RNA

open access: yesFEBS Open Bio, EarlyView.
Plasma extracellular vesicles were captured with WGA‐conjugated magnetic beads and profiled for RNA biomarkers. A three‐RNA panel (NM_024955, NR_047469, and NR_002564) distinguished prostate cancer from healthy controls and benign prostatic hyperplasia, supporting a simple, noninvasive approach to improve prostate cancer detection.
Hanping Wei, Haoran Wu, Wei Feng
wiley   +1 more source

Deep learning-based multiple particle tracking in complex system

open access: yesAIP Advances
This paper presents an innovative approach for multiple particle tracking within complex systems, utilizing convolutional neural networks in conjunction with multi-output models.
Xiaoming Xu, Jianjun Wei, Sheng Sang
doaj   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

open access: yesFEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen   +5 more
wiley   +1 more source

PARTICLE FILTER-BASED OBJECT TRACKING USING JOINT FEATURES OF COLOR AND LOCAL BINARY PATTERN HISTOGRAM FOURIER

open access: yesJurnal Ilmiah Kursor: Menuju Solusi Teknologi Informasi, 2015
Object tracking is defined as the problem of estimating object location in image sequences. In general, the problems of object tracking in real time and complex environtment are affected by many uncertainty.
Dewa Made Wiharta   +2 more
doaj   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

open access: yesFEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh   +2 more
wiley   +1 more source

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