Results 41 to 50 of about 326,331 (291)
Frontiers in Aging Neuroscience, 2021 A patch-clamp recording in slices generated from the brain or the spinal cord has facilitated the exploration of neuronal circuits and the molecular mechanisms underlying neurological disorders.
Yang Li +5 more
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Protocol to prepare mouse spinal cord for patch-clamp and histology experiments
STAR Protocols, 2023 Summary: The spinal cord is a part of the central nervous system located within the spinal canal of the vertebrae. Here, we present a protocol to prepare mouse spinal cord sections for patch-clamp and histology experiments.
Sang-Hyeon Ju, Jong-Woo Sohn
doaj +1 more source
Whole-GUV patch-clamping [PDF]
Proceedings of the National Academy of Sciences, 2016 Significance Although membrane composition and tension modulate the activity of ion channels and transporters, this protein–membrane coupling has been challenging to study due to the difficulty of controlling membrane properties in cells and technical limitations of existing in vitro systems. This work demonstrates that the whole-cell
Garten, Matthias +5 more
openaire +3 more sources
Frontiers in Physiology, 2023 Human-induced stem cell-derived cardiomyocytes (hiPSC-CMs) are a valuable tool for studying development, pharmacology, and (inherited) arrhythmias. Unfortunately, hiPSC-CMs are depolarized and spontaneously active, even the working cardiomyocyte subtypes
Arie O. Verkerk +2 more
doaj +1 more source
Protocol for pressure-clamped patch-clamp recording at the node of Ranvier of rat myelinated nerves
STAR Protocols, 2021 Summary: The patch-clamp recording technique is indispensable for studying ion channel functions of cells but is challenging to apply to the node of Ranvier, a key site where action potentials are conducted along myelinated nerves.
Hirosato Kanda +3 more
doaj +1 more source
Planar Patch Clamp and Techniques of Patch-Clamp Spectroscopy in Antimicrobial Therapy
Austin Journal of Pharmacology and Therapeutics, 2023 Planar patch-clamp is an effective screening tool in pharmacology, institutionalized in molecular microbiology over 15 years ago [1]. The creation of planar polymer patch-clamp electrodes and silicon substrates in the 2000s [2,3] and the creation of microfluidic chips and perfusable chambers corresponding to this pharmacological screening technology ...
Orehow F, Adamovic E, Gradow O
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Advanced Engineering Materials, EarlyView.A numerical–experimental framework is developed for characterizing multi‐matrix fiber‐reinforced polymers (MM‐FRPs) combining epoxy and polyurethane matrices. Harmonic bending tests are integrated with finite element model updating (FEMU) to simultaneously identify elastic and viscoelastic material parameters.
Rodrigo M. Dartora +4 more
wiley +1 more source
Molecular Pain, 2015 Mammals use tactile end-organs to perform sensory tasks such as environmental exploration, social interaction, and tactile discrimination. However, cellular and molecular mechanisms underlying tactile transduction in tactile end-organs remain poorly ...
Ryo Ikeda +3 more
doaj +1 more source
Nanoscale-targeted patch-clamp recordings of functional presynaptic ion channels [PDF]
, 2013 Important modulatory roles have been attributed to presynaptic NMDA receptors (NMDARs) located on cerebellar interneuron terminals. Evidence supporting a presynaptic location includes an increase in the frequency of mini events following the application ...
Caldwell, M +4 more
core
High conductance anion channel in Schwann cell vesicles from rat spinal roots [PDF]
, 1992 Potassium uptake, possibly together with chloride, is one of the presumed functions of Schwann cells in the peripheral nervous system. However, the presence of chloride channels has not been demonstrated in adult Schwann cells.
Quasthoff, Stefan +5 more
core +1 more source