Results 271 to 280 of about 183,075 (317)
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1995
In this chapter, I shall explain how to set-up a patch-clamp technique. The aim is to guide researchers in their initial steps in the field of electrophysiology, describing the instrumentation needed, as well as the technique itself. This chapter has been written assuming that the reader has a basic knowledge of electrophysiology.
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In this chapter, I shall explain how to set-up a patch-clamp technique. The aim is to guide researchers in their initial steps in the field of electrophysiology, describing the instrumentation needed, as well as the technique itself. This chapter has been written assuming that the reader has a basic knowledge of electrophysiology.
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The Patch Clamp Technique in Ion Channel Research
Current Pharmaceutical Biotechnology, 2004To understand the pathogenesis of a given ion channel disorder, knowledge of the mutation alone is insufficient, instead, the description of the associated functional defect is decisive. The patch clamp technique enables to achieve this both in native tissue as well as heterologous expression systems. By this technique, structure-function relationships
K, Jurkat-Rott, F, Lehmann-Horn
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Patch-clamp technique in renal physiology
American Journal of Physiology-Renal Physiology, 1986The patch-clamp technique of Neher and Sakmann and their colleagues has been widely used over the last 5 years to investigate ion channels in excitable tissues. More recently, it has become useful as a tool to study channels involved in transepithelial ion transport.
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Patch clamp techniques: an overview.
Methods in enzymology, 1992This chapter provides an overview of the patch clamp techniques. The patch clamp technique provides the experimental means for merging the tools of modern molecular and cellular biology with those of electrophysiology. Using various recording configurations, it is possible to dissect the mechanisms of channel modulation.
M, Cahalan, E, Neher
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Yakubutsu, seishin, kodo = Japanese journal of psychopharmacology, 1992
We first describe how to set up the system for patch-clamp recording and carry out the experiments with 4 different modes of conventional patch-clamp technique: cell-attached, whole-cell, inside-out and outside-out configurations. Thereafter, we mention about an improved 'nystatin perforated' patch-clamp technique which dissolves the fault of ...
N, Akaike, T, Shirasaki
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We first describe how to set up the system for patch-clamp recording and carry out the experiments with 4 different modes of conventional patch-clamp technique: cell-attached, whole-cell, inside-out and outside-out configurations. Thereafter, we mention about an improved 'nystatin perforated' patch-clamp technique which dissolves the fault of ...
N, Akaike, T, Shirasaki
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Patch-Clamp Technique in ESC-Derived Cardiomyocytes
2014ESC-derived cardiomyocytes are excitable cells that express many of the ion channels also found in adult cardiomyocytes. The patch-clamp technique is a powerful technique to characterize both the electrophysiological properties of excitable cells as well as the underlying ion channel currents responsible for these electrophysiological properties.
Jie, Liu, Peter H, Backx
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Perforated Patch-Clamp Technique
2003Application of the conventional whole-cell patch-clamp method leads to a replacement of the intracellular fluid with the intracellular pipet solution. The speed of this replacement or dialysis depends on the cell volume and electrode tip diameter. Although this mechanism can be used advantageously in many experiments, there are conditions where such a ...
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Patch Clamp Technique and Applications
2017There are two main modes in patch clamp recordings: voltage clamp and current clamp. In the voltage clamp mode, the membrane voltage is controlled by the amplifier through the recording pipette and the corresponding current through the pipette is measured.
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Patch-Clamp Technique in Brain Slices
2003The technique of patch-clamp recording in brain slices is applicable to a large variety of cell types in slices from nearly all areas of the central nervous system (CNS) in animals at many different stages of development (Blanton et al., 1989; Edwards et al., 1989; Konnerth, 1990).
T. D. Plant, J. Eilers, A. Konnerth
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