Results 91 to 100 of about 154,598 (309)

The effects of cryopreservation on PBMCs transcriptome profile

Frontiers in Immunology
Cryopreservation is a key method for long-term storage of biological specimens. The development of optimal cryopreservation and recovery conditions will minimize storage-related damage. The effect of the cryopreservation and recovery condition we used on peripheral blood mononuclear cells (PBMCs) has previously been evaluated using microarray analysis.
Xuan, Li, Qian, Chen, Jeanette, Higgins, Kyndal, Cook, Michael W, Baseler, Tomozumi, Imamichi, Weizhong, Chang   +6 more
openaire   +2 more sources

HNRNPD Induces Radioresistance in Nasopharyngeal Carcinoma by Sequestering GRAMD4 mRNA in Stress Granules

Advanced Science, EarlyView.
HNRNPD promotes radioresistance in nasopharyngeal carcinoma by enhancing stress granule assembly and sequestering GRAMD4 mRNA. This suppresses GRAMD4 translation and inhibits mitochondrial apoptosis. Targeting the integrated stress response with ISRIB restores GRAMD4 expression and sensitizes tumors to radiotherapy, revealing a translational control ...
Yingzi Li, Tong Xiang, Yuanyuan Liu, Desheng Weng, Jingjing Zhao, Hao Chen, Yan Tang, Songzuo Xie, Hao Zhang, Jun Luo, Xinyi Yang, Qiuzhong Pan, Muping Di, Jianchuan Xia   +13 more
wiley   +1 more source

Preparation of PBMCs

Fachrepositorium Lebenswissenschaften, 2021
VACCELERATE (Projekt), Kelly, Christine, Garcia Leon, Alejandro Abner   +2 more
openaire   +1 more source

PBMCs from patients responding to adalimumab and etanercept produce higher levels of TNF-α and sTNFRII than PBMCs from patients responding to infliximab.

, 2016
(A) TNF-α levels were quantified in the supernatant of peripheral blood mononuclear cells (PBMCs) in the absence or in the presence of lipopolysaccharide (LPS) for 24 hours.
Federica Ferrari (3391226), Marcello Pinti (295127), Andrea Cossarizza (295123), Marco Manfredini (3391214), Milena Nasi (295128), Giuseppe Albertini (3391220), Regina Bartolomeo (3391223), Elena Bianchini (686335), Carlo Salvarani (412600), Lara Gibellini (117504), Giovanni Pellacani (266366), Sara De Biasi (295129), Anna Iannone (2339785), Antonella Fabiano (3391217), Tommaso Trenti (179891)   +14 more
core   +1 more source

Enhancing CAR‐T Cell Efficacy in Solid Tumors by Inhibiting CCL5/VEGF‐Mediated Angiogenesis

Advanced Science, EarlyView.
This study reveals that CAR‐T cells in solid tumors produce CCL5, which paradoxically induces VEGF and angiogenesis to promote tumor growth. Blocking CCL5/VEGF signaling—through gene knockout, or the CCR5 inhibitor maraviroc—significantly enhances the antitumor efficacy of CAR‑T therapy (the diagram was created in Biorender).
Shishuo Sun, Qihong Li, Bixi Wang, Gang Wang, Ying Xue, Yibing Liang, Chenhe Hu, Zhenyu Wang, Xiaoge Gao, Haiheng Xu, Jun Song, Hailong Li, Liantao Li, Xiaoxiao Liu, Patrick Ming‐Kuen Tang, Qing Zhang   +15 more
wiley   +1 more source

MALDI-TOF MS monitoring of PBMC activation status in sepsis

BMC Infectious Diseases, 2018
Background MALDI-TOF mass spectrometry (MS) on whole cells enables the detection of different cell types and cell activation. Here, we wondered whether this approach would be useful to investigate the host response in sepsis.
Aurélie Daumas, Julie Alingrin, Richard Ouedraogo, Patrick Villani, Marc Leone, Jean-Louis Mege   +5 more
doaj   +1 more source

Targeting METTL3 Attenuates Thyroid Inflammatory Injury by Restoring Th17/Treg Balance through a YTHDC2‐m6A‐Dependent KDR/VEGFA Loop

Advanced Science, EarlyView.
METTL3‐mediated m6A modification stabilizes KDR/Kdr mRNA through YTHDC2, amplifying the VEGFA‐KDR feedback loop and disturbing Th17/Treg balance. This pathway promotes persistent inflammation and tissue damage in patients with AIT, and inhibiting the METTL3‐KDR axis effectively disrupts this circuit, alleviating thyroid tissue injury and disease ...
Qingyi Hu, Huan Liu, Anwen Ren, Zimei Tang, Jie Tan, Wen Yang, Jie Ming, Tao Huang   +7 more
wiley   +1 more source

The expression of GARP on CD4⁺ T cells in freshly isolated PBMCs and CD3/28-sitmulated PBMCs.

, 2014
Bood samples were collected from patients with CPS (n = 20), CSA (n = 17) and ACS (n = 37, AMI (n = 18), and UA (n = 19)). PBMCs were freshly isolated or stimulated with CD3/CD28 for 24 h.
Ze-Peng Ren (523675), Xiao-Bo Mao (341202), Wei Zhang (405), Qin-Wei Ji (523677), Qiu-Tang Zeng (168027), Bang-Wei Wu (523676), Yu-Cheng Zhong (523672), Zheng-Feng Zhu (205005), Peng-Fei Zhu (523674), Xiang Wang (127867), Kun-Wu Yu (523673), Kai Meng (523671), Liang Qi (484925)   +12 more
core   +1 more source

Radiotherapy Enhances the Oncolytic Efficacy of the Novel Oncolytic Herpesvirus VG161 and Amplifies Its Antitumor Immunity in Breast Cancer

Advanced Science, EarlyView.
RT promotes VG161 replication in BC and its immunostimulatory transgenes expression, which is mediated by the upregulation of GADD34 and HVEM caused by RT. The combination therapy with VG161 and RT increases the abundances of tumor‐infiltrating lymphocytes and elicits potent systemic antitumor immunity, thereby effectively inhibiting local tumors and ...
Lijuan Lyu, Ming Yi, Ji Chen, Zeda Zhao, Ruocen Liao, Yingnan Wang, Shengtao Hu, Jun Ding, Ronghua Zhao, William Jia, Yinan Shen, Yongchang Wei, Kongming Wu, Zhijun Dai   +13 more
wiley   +1 more source

Activation of peroxisome proliferator-activated receptor alpha in human peripheral blood mononuclear cells reveals an individual gene expression profile response

BMC Genomics, 2008
Background Peripheral blood mononuclear cells (PBMCs) are relatively easily obtainable cells in humans. Gene expression profiles of PBMCs have been shown to reflect the pathological and physiological state of a person.
Afman Lydia A, Bouwens Mark, Müller Michael   +2 more
doaj   +1 more source