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Internal Validation of a Real-Time qPCR Kit following the UNE/EN ISO/IEC 17025:2005 for Detection of the Re-Emerging Monkeypox virus

open access: yesDiagnostics, 2023
Human mpox is caused by the Monkeypox virus, a microorganism closely related to the Variola virus, both belonging to the Orthopoxvirus genus. Mpox had been considered a rare disease until a global outbreak occurred in 2022. People infected with the virus
Antonio Martínez-Murcia   +4 more
doaj   +1 more source

Screening of 50 Emerging Contaminants in Wastewater by Micro-direct-injection UPLC-MS/MS [PDF]

open access: yesZhipu Xuebao, 2023
In recent years, with the increasingly serious problem of environmental pollution, people′s awareness of environmental protection has gradually strengthened.
REN Hang1,2   +6 more
doaj   +1 more source

Internal Validation of the ASFV MONODOSE dtec-qPCR Kit for African Swine Fever Virus Detection under the UNE-EN ISO/IEC 17025:2005 Criteria

open access: yesVeterinary Sciences, 2023
African swine fever virus is considered an emerging virus that causes African swine fever, a disease characterised by high mortality and elevated transmission rates and that, as it is for most other viral diseases, cannot be treated with specific drugs ...
Gema Bru   +4 more
doaj   +1 more source

SARS-CoV-2 Variants Identification; A Fast and Affordable Strategy Based on Partial S-Gene Targeted PCR Sequencing

open access: yesViruses, 2022
A considerable number of new SARS-CoV-2 lineages have emerged since the first COVID-19 cases were reported in Wuhan. As a few variants showed higher COVID-19 disease transmissibility and the ability to escape from immune responses, surveillance became ...
Antonio Martínez-Murcia   +3 more
doaj   +1 more source

The PCR Suite [PDF]

open access: yesBioinformatics, 2004
Abstract Summary: The web application PCR Suite is an extension of the primer design program Primer3. It allows the design of primer sets encompassing single nucleotide polymorphisms, all exons of a single gene, all open reading frames in a list of cDNAs or the creation of overlapping PCR products.
Marijke J. van Baren, Peter Heutink
openaire   +3 more sources

Quantitative PCR and RT-PCR in virology. [PDF]

open access: yesGenome Research, 1993
In the last few years, molecular hybridization methods have been used extensively in basic and applied virology because of their technical flexibility and high specificity. Using these techniques, the detection of DNA and RNA viruses directly from clinical specimens, the analysis of the specific transcriptional activity of viral genes in vitro and in ...
CLEMENTI M   +5 more
openaire   +4 more sources

Intensive diagnostic management of coronavirus disease 2019 (COVID-19) in academic settings in Japan: challenge and future

open access: yesInflammation and Regeneration, 2020
Coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), first emerged in Wuhan, China, and has spread globally to most countries. In Japan, the first COVID-19 patient was identified on January 15, 2020.
Tokio Hoshina   +8 more
doaj   +1 more source

The Model for Storing Tokens in Local Storage (Cookies) Using JSON Web Token (JWT) with HMAC (Hash-based Message Authentication Code) in E-Learning Systems

open access: yesJournal of Applied Engineering and Technological Science, 2022
E-learning is a technology that may be used in the learning process to improve not only the distribution of learning materials but also the ability of learners to modify their abilities of various competencies contained in a database.
Syabdan Dalimunthe   +2 more
doaj   +1 more source

Correlation of Chest CT and RT-PCR Testing in Coronavirus Disease 2019 (COVID-19) in China: A Report of 1014 Cases

open access: yesRadiology, 2020
Background Chest CT is used for diagnosis of 2019 novel coronavirus disease (COVID-19), as an important complement to the reverse-transcription polymerase chain reaction (RT-PCR) tests. Purpose To investigate the diagnostic value and consistency of chest
T. Ai   +8 more
semanticscholar   +1 more source

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