Results 1 to 10 of about 3,602,392 (295)
Abstract Summary: The web application PCR Suite is an extension of the primer design program Primer3. It allows the design of primer sets encompassing single nucleotide polymorphisms, all exons of a single gene, all open reading frames in a list of cDNAs or the creation of overlapping PCR products.
Marijke J. van Baren, Peter Heutink
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Quantitative PCR and RT-PCR in virology. [PDF]
In the last few years, molecular hybridization methods have been used extensively in basic and applied virology because of their technical flexibility and high specificity. Using these techniques, the detection of DNA and RNA viruses directly from clinical specimens, the analysis of the specific transcriptional activity of viral genes in vitro and in ...
CLEMENTI M+5 more
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The identification of predefined mutations expected to be present in a minor fraction of a cell population is important for a variety of basic research and clinical applications. Here, we describe an approach for transforming the exponential, analog nature of the PCR into a linear, digital signal suitable for this purpose. Single molecules
Bert Vogelstein, Kenneth W. Kinzler
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Abstract Summary: We present an algorithm that uses public sequence data to predict PCR products. The algorithm is implemented as a CGI script. Output is compared to real-world PCR. Availability: Perl code and instructions for installation are freely available over the internet at http://www.sci.muni.cz/LMFR/vpcr.html
M, Lexa, J, Horak, B, Brzobohaty
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What forms the chunks in a subject's performance? Lessons from the CHREST computational model of learning [PDF]
Computational models of learning provide an alternative technique for identifying the number and type of chunks used by a subject in a specific task. Results from applying CHREST to chess expertise support the theoretical framework of Cowan and a limit ...
Cheng, PCH, Gobet, F, Lane, PCR
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Modelling the relationship between visual short-term memory capacity and recall ability [PDF]
Previous cognitive modelling work has suggested that the decline of short-term memory (STM) capacity is the dominant factor of age-related decline on recall ability. We report the results of a set of experiments investigating in further detail the effect
Gobet, F, Lane, PCR, Smith, RL
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PCR for the detection of pathogens in neonatal early onset sepsis. [PDF]
BACKGROUND: A large proportion of neonates are treated for presumed bacterial sepsis with broad spectrum antibiotics even though their blood cultures subsequently show no growth. This study aimed to investigate PCR-based methods to identify pathogens not
Bedford Russell, A+8 more
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A new approach to profiling taphonomic history through bone fracture analysis, with an example application to the Linearbandkeramik site of Ludwinowo 7. [PDF]
This is the author accepted manuscript. The final version is available from the publisher via the DOI in this record.This paper presents a new method of assessing and displaying taphonomic history through detailed bone fracture analysis.
Johnson, EV, Outram, AK, Parmenter, PCR
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Westward propagating twin gyres in the equatorial Indian Ocean [PDF]
A reduced-gravity (11/2-layer) model forced by daily climatological winds simulates twin, anticyclonic gyres, which propagate westward on either side of the equator.
Deo, AA+3 more
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Digital PCR as a tool to measure HIV persistence [PDF]
Although antiretroviral therapy is able to suppress HIV replication in infected patients, the virus persists and rebounds when treatment is stopped.
Bosman, Kobus+4 more
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