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Water Research, 2005
We tested three PCR based methodologies to detect adenoviruses associated with cultivated oysters. Conventional-PCR, nested-PCR, and integrated cell culture-PCR (ICC/PCR) were first optimized using oysters seeded with know amounts of Adenovirus serotype 5 (Ad5).
Caroline Rigotto +2 more
exaly +3 more sources
We tested three PCR based methodologies to detect adenoviruses associated with cultivated oysters. Conventional-PCR, nested-PCR, and integrated cell culture-PCR (ICC/PCR) were first optimized using oysters seeded with know amounts of Adenovirus serotype 5 (Ad5).
Caroline Rigotto +2 more
exaly +3 more sources
Analytical and Bioanalytical Chemistry, 2003
Miniaturization of genetic tests has become an important goal. This review surveys the current progress towards the miniaturization of tests based on the polymerase chain reaction (PCR). It examines the different types of PCR microchip designs, fabrication methods,and the components of a microchip PCR device. It also discusses the problems attributable
L J, Kricka, P, Wilding
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Miniaturization of genetic tests has become an important goal. This review surveys the current progress towards the miniaturization of tests based on the polymerase chain reaction (PCR). It examines the different types of PCR microchip designs, fabrication methods,and the components of a microchip PCR device. It also discusses the problems attributable
L J, Kricka, P, Wilding
openaire +2 more sources
2013
Colony PCR is a method for rapidly screening colonies of yeast or bacteria that have grown up on selective media following a transformation step, to verify that the desired genetic construct is present, or to amplify a portion of the construct.
Bergkessel, Megan, Guthrie, Christine
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Colony PCR is a method for rapidly screening colonies of yeast or bacteria that have grown up on selective media following a transformation step, to verify that the desired genetic construct is present, or to amplify a portion of the construct.
Bergkessel, Megan, Guthrie, Christine
openaire +3 more sources
cclm, 2000
Abstract The classic molecular biology methods like Northern or Southern blot analyse non-amplified DNA or RNA, but need large amounts of nucleic acids, in many instances from tissues or cells that are heterogeneous. In contrast, polymerase chain reaction (PCR)-based techniques allow us to obtain genetic information through the specific ...
R, Jung, K, Soondrum, M, Neumaier
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Abstract The classic molecular biology methods like Northern or Southern blot analyse non-amplified DNA or RNA, but need large amounts of nucleic acids, in many instances from tissues or cells that are heterogeneous. In contrast, polymerase chain reaction (PCR)-based techniques allow us to obtain genetic information through the specific ...
R, Jung, K, Soondrum, M, Neumaier
openaire +2 more sources
Reverse-Transcription PCR (RT-PCR)
2013RT-PCR is commonly used to test for genetic diseases and to characterize gene expression in various tissue types, cell types, and over developmental time courses. This serves as a form of expression profiling, but typically as a candidate approach. RT-PCR is also commonly used to clone cDNAs for further use with other molecular biology techniques (e.g.,
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2016
Real-time Apta-PCR is a methodology that can be used for a wide variety of applications ranging from food quality control to clinical diagnostics. This method takes advantage of the combination of the sensitivity of nucleic acid amplification with the selectivity of aptamers.
Alessandro, Pinto +5 more
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Real-time Apta-PCR is a methodology that can be used for a wide variety of applications ranging from food quality control to clinical diagnostics. This method takes advantage of the combination of the sensitivity of nucleic acid amplification with the selectivity of aptamers.
Alessandro, Pinto +5 more
openaire +2 more sources
European Journal of Cancer, 1992
cytotoxicity in human ovarian cancer cell lines by glutathione depletion. Cancer Res 1984,44,5427-5431. 51. Hansson J, Berhane K, Castro VM, Jungnelius U, Mannervik B, Ringborg U. Sensitization of human melanoma cells to the cytotoxic effects of melphalan by the glutathione transferase inhibitor e-&crynic acid. CancerRes 1991,51,94-98. 52.
R A, Eeles, W, Warren, A, Stamps
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cytotoxicity in human ovarian cancer cell lines by glutathione depletion. Cancer Res 1984,44,5427-5431. 51. Hansson J, Berhane K, Castro VM, Jungnelius U, Mannervik B, Ringborg U. Sensitization of human melanoma cells to the cytotoxic effects of melphalan by the glutathione transferase inhibitor e-&crynic acid. CancerRes 1991,51,94-98. 52.
R A, Eeles, W, Warren, A, Stamps
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2017
Escherichia coli and Saccharomyces cerevisiae are currently the two most important organisms in synthetic biology. E.coli is almost always used for fundamental DNA manipulation while yeast is the simplest host system for studying eukaryotic gene expression and performing large scale DNA assembly.
Azevedo, Flávio +2 more
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Escherichia coli and Saccharomyces cerevisiae are currently the two most important organisms in synthetic biology. E.coli is almost always used for fundamental DNA manipulation while yeast is the simplest host system for studying eukaryotic gene expression and performing large scale DNA assembly.
Azevedo, Flávio +2 more
openaire +3 more sources
The impact of the PCR plateau phase on quantitative PCR
Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression, 1994The quantitative use of the polymerase chain reaction (PCR) is often compromised by the variability of the amplification. The most useful system for quantitation by PCR involves the use of controls which are almost identical to the target and which can be amplified using the same primers as the sequences of interest.
C, Morrison, F, Gannon
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