Results 41 to 50 of about 2,958,232 (316)

Accurate quantification of microorganisms in PCR-inhibiting environmental DNA extracts by a novel Internal Amplification Control approach using Biotrove OpenArrays [PDF]

, 2009
PCR-based detection assays are prone to inhibition by substances present in environmental samples, thereby potentially leading to inaccurate target quantification or false-negative results.
Gent-Pelzer, M. van, Gent-Pelzer, M.P.E., van, Van Doorn, R., Doorn, R. van, Speksnijder, A., Speksnijder, A.G.C.L., Klerks, M.M., Schoen, C.D., van Gent-Pelzer, M., Klerks, M., Gent-Pelzer van, M., Doorn, R., van, Kowalchuk, G.A., van Gent-Pelzer, M.P.E.   +13 more
core   +2 more sources

Extraction of HCV-RNA from Plasma Samples: Development towards Semiautomation

International Journal of Analytical Chemistry, 2015
A semiautomated extraction protocol of HCV-RNA using Favorgen RNA extraction kit has been developed. The kit provided protocol was modified by replacing manual spin steps with vacuum filtration. The assay performance was evaluated by real-time qPCR based
Imran Amin, Tania Jabbar, Fawad Niazi, Muhammad Saeed Akhtar   +3 more
doaj   +1 more source

Biphasic Clinical Course of Pediatric Cytomegalovirus‐Associated Immune Thrombocytopenia: Early Poor Treatment Response but Favorable Long‐Term Outcomes

Pediatric Blood &Cancer, EarlyView.
ABSTRACT Background Cytomegalovirus (CMV) is a recognized trigger of immune thrombocytopenia (ITP); however, its incidence and impact on disease course in children remain controversial. Therefore, we aimed to characterize the clinical course of pediatric patients with CMV‐associated ITP.
Oded Gilad, Ohad Atia, Osama Tanous, Dafna Brik Simon, Michal Dvori, Miriam Cohen, Tracie A. Goldberg, Efraim Bilavsky, Joanne Yacobovich, Orna Steinberg‐Shemer   +9 more
wiley   +1 more source

PCR-based screening for the HGT candidates.

, 2021
(a) Results of PCR amplification with the AsBGNL/LpBGNL_F and AsBGNL_R primers (top panel), and the AsFMOL_con_f1_AstII and AsFMOL_con_r1_AstII primers (second panel).
Yugo Watanabe (11419734), Hiroshi Shinozuka (6181910), German C. Spangenberg (7869902)   +2 more
core   +1 more source

An isoform of 14‐3‐3 protein regulates transbilayer lipid movement at the plasma membrane

FEBS Letters, EarlyView.
Loss of 14‐3‐3ζ in CHO cells confers resistance to exogenous phosphatidylserine (PS) and impairs endocytosis‐independent inward flip‐flop of fluorescent PS at the plasma membrane. RNAi‐mediated knockdown reproduces this defect, while no additive effect is seen in ATP11C‐deficient cells.
Akiko Yamaji‐Hasegawa, Akira Hattori, Masafumi Tsujimoto, Toshihide Kobayashi   +3 more
wiley   +1 more source

Validation of a DIVA qPCR Duplex Assay to Differentiate Primun Salmonella T Vaccine from Salmonella enterica subsp. enterica Serovar Typhimurium Wild Strains

Applied Sciences
Salmonella enterica subsp. enterica serovar Typhimurium is an important foodborne pathogen, and poultry products are a major source of human infection.
Antonio Martínez-Murcia, Aaron Navarro, Adrián García-Sirera, Paloma Romero, Caridad Miró-Pina, Jesús María Rubio, Ante Blazevic   +6 more
doaj   +1 more source

Septin 9 PB domains coordinate centrosome positioning and microtubule acetylation to control epithelial polarity

FEBS Letters, EarlyView.
Septin 9 polybasic domains couple phosphoinositide‐rich membrane binding to centrosome positioning, Golgi organization, and microtubule acetylation to control epithelial polarity. Their loss disrupts this axis, causing centrosome mispositioning, Golgi fragmentation, reduced microtubule acetylation, and polarity inversion via upregulation of the ...
Ting ting Cai, Mohyeddine Omrane, Nassima Benzoubir, Didier Samuel, Ama Gassama‐Diagne   +4 more
wiley   +1 more source

Desenvolvimento de iniciadores e sondas para detecção de cry1A.105 e cry2Ab2 e aplicação de PCR e PCR em tempo real para detecção de OGM em alimentos [PDF]

, 2011
Tese (doutorado) - Universidade Federal de Santa Catarina, Centro de Ciências Agrárias, Programa de Pós-Graduação em Ciência dos Alimentos, Florianópolis, 2011Reação em Cadeia da Polimerase (PCR) e PCR quantitativa em tempo real são usadas para detecção ...
Dinon, Andréia Zilio
core  

Investigating transcription factor dynamics in health and disease using FRAP

FEBS Letters, EarlyView.
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj, Carolina Vazquez Garzon, Jose Joaquin Velasco, Janine N. Post   +3 more
wiley   +1 more source

Association between interleukin-1 receptor polymorphism and human herpesvirus 8 among lymphoma patients

Medical Journal of Babylon, 2023
Background: Human herpesvirus 8 (HHV-8) stimulates lymphoproliferation, by activating the signaling pathway of the interleukin-6 receptor, along with several other regulatory mechanisms.
Ameer J Abbas Al-Jawdhari, Shakir H Mohammed Al-Alwany   +1 more
doaj   +1 more source