Results 61 to 70 of about 2,958,232 (316)

Multiplex PCR Assay for unequivocal differentiation of Actinobacillus pleuropneumoniae serovars 1 to 3, 5 to 8, 10, and 12 [PDF]

, 2014
This work was supported by a Longer and Larger (LoLa) grant from the Biotechnology and Biological Sciences Research Council (grant numbers BB/G020744/1, BB/G019177/1, BB/G019274/1, and BB/G003203/1) and the United Kingdom Department for Environment, Food
Maskell, Duncan J, Holden, M T, Chaudhuri, RR, Tucker, AW, Rycroft, Andrew N, Rycroft, AN, Angen, O, Tucker, A W, Weinert, LA, Weinert, Lucy A, Wren, Brendan W, Cambridge, Holden, Matthew T, Bossé, J T, BRaDP1T Consortium, LSHTM, Chaudhuri, R R, Bossé, Janine T, Angen, Oystein, Tucker, Alexander W, Norwegian Vet Instit, Chaudhuri, Roy R, Langford, PR, Holden, MT, Angen, Øystein, Rycroft, A N, Bosse, JT, Holden, Matt T, Wellcome Trust Sanger Institute, Wren, BW, Imperial College London, Williamson, Susanna M, Williamson, S M, Maskell, D J, Wren, B W, Williamson, SM, Weinert, L A, Li, Yanwen, Langford, P R, AHVLA, Maskell, DJ, Langford, Paul R, Holden, Matthew, Li, Y, Angen, Ø   +44 more
core   +1 more source

PCR-based genotyping of Exoc7 flox KI design.

, 2022
(a) PCR-RFLP design to validate LoxP KI alleles. The AscI and EcoRV digest the restriction sites adjacent to Left LoxP and Right LoxP, respectively. The arrows represent PCR primers for the digested DNA fragments, including PCR product sizes.
Michito Hamada (11965042), Masafumi Muratani (4760067), Natsumi Iki (11965030), Shinya Ayabe (3592004), Tra Thi Huong Dinh (11965039), Akihiro Kuno (4733382), Atsushi Yoshiki (102433), Kotaro Sakamoto (4019420), Sayaka R. Suzuki (11965018), Arata Wakimoto (11965024), Yoko Daitoku (11965036), Satoru Takahashi (32871), Seiya Mizuno (6169970), Natsuki Mikami (11965027), Kanako Kato (5145539), Yuko Hamada (142543), Megumi Takemura (11965033), Kazuya Murata (4043480), Yoshihisa Ikeda (564228), Miyuki Ishida (7829756), Fumihiro Sugiyama (759758), Yoko Tanimoto (6158447), Kento Morimoto (11965021)   +22 more
core   +1 more source

Partial depletion of plasminogen activator inhibitor‐1 decreases subcutaneous fat cell hypertrophy and liver cholesterol in high‐fat‐fed female mice

FEBS Letters, EarlyView.
Obesity raises blood levels of PAI‐1, a protein linked to metabolic dysfunction‐associated steatotic liver disease in people with obesity. In female mice fed a high‐fat diet, partially lowering PAI‐1 led to smaller subcutaneous fat cells and lower liver cholesterol, without changing body weight or insulin sensitivity.
Claudia E. Ramirez Bustamante, Robert Sharp, Pradip K. Saha, Elizabeth S. Anaya, Elizabeth L. Lieu, Evelyn L. de Groot, Pooja Patel, Mi Jeong Heo, Aaron R. Cox, Kang Ho Kim, Sean M. Hartig   +10 more
wiley   +1 more source

COVID-19 in Pakistan

BioMedica, 2020
Coronavirus disease-2019 (COVID-19) outbreak was first declared as emergency on January 30th2020 by World Health Organization (WHO). The Government of Pakistan presented a National action plan, which aimed to inhibit the spread of this Corona virus and ...
Ghazala Rubi, Irfan Malik, Zainab Javaid
doaj   +1 more source

Functional comparison of EncB and EncC cargo proteins in iron storage within the Myxococcus xanthus encapsulin

FEBS Letters, EarlyView.
Encapsulins are protein nanocompartments that play an important role in iron storage. In the Myxococcus xanthus encapsulin system, two cargo proteins called EncB and EncC contribute to iron mineralization. Here, we show that EncB and EncC generate iron‐containing minerals with distinct chemical compositions, suggesting that the composition of stored ...
Harry B. McDowell, Thomas Walther, Egbert Hoiczyk   +2 more
wiley   +1 more source

Comparison of PCR and microscopy for detection of Cryptosporidium parvum in human fecal specimens: clinical trial [PDF]

, 1998
PCR technology offers alternatives to conventional diagnosis of Cryptosporidium for both clinical and environmental samples. We compared microscopic examination by a conventional acid-fast staining procedure with a recently developed PCR test that can ...
Thompson, R.C.A., Dwyer, B.W., Morgan, U.M., Richomme, G., Forbes, D.A., Pallant, L.   +5 more
core  

A new PCR-based approach indicates the range of Clonorchis sinensis now extends to central Thailand [PDF]

, 2009
Differentiation of the fish-borne trematodes belonging to the Opisthorchiidae, Heterophyidae and Lecithodendriidae is important from a clinical and epidemiological perspective, yet it is impossible to do using conventional coprological techniques, as the
Thompson, R.C.A., K Darwin Murrell, Leelayoova, S, Murrell, K. Darwin, Cribb, Thomas, Cribb, T, Thomas Cribb, Cribb, T., Macaranas, J, Thompson R. C. Andrew, Julie Macaranas, Thompson, RCA, R C Andrew Thompson, Saovanee Leelayoova, Mungthin, M, Macaranas, Julie, Mungthin, M., Leelayoova, Saovanee, Thompson, R. C. Andrew, Macaranas Julie, Murrell K. Darwin, Traub, Rebecca J., Mungthin, Mathirut, Mathirut Mungthin, Traub, RJ, Murrell, KD, Cribb Thomas, Macaranas, J., Traub, R.J., Rebecca J Traub, Leelayoova, S., Traub Rebecca J., Mungthin Mathirut, Murrell, K.D., Leelayoova Saovanee   +34 more
core   +2 more sources

Structural and biochemical analysis of a B12 superbinder

FEBS Letters, EarlyView.
BtuG proteins are vitamin B12 scavengers in Bacteroides thetaiotaomicron, a dominant human gut bacterium. We present crystal structures of three BtuG homologs bound to cobalamin and its precursor cobinamide, revealing picomolar binding affinities, among the highest known for any natural protein.
Jose M. Martinez Felices, Jacob J. Whittaker, Dirk J. Slotboom, Albert Guskov   +3 more
wiley   +1 more source

Groundtruthing next-gen sequencing for microbial ecology-biases and errors in community structure estimates from PCR amplicon pyrosequencing [PDF]

, 2012
Analysis of microbial communities by high-throughput pyrosequencing of SSU rRNA gene PCR amplicons has transformed microbial ecology research and led to the observation that many communities contain a diverse assortment of rare taxa-a phenomenon termed ...
Polson, Shawn W., Shawn W. Polson (139348), Craig W. Herbold (139346), Charles K Lee, Ian R McDonald, S Craig Cary, K Eric Wommack, K. Eric Wommack (139352), Charles K. Lee (139341), Ian R. McDonald (139355), Herbold, Craig W., Lee, Charles Kai-Wu, Shawn W Polson, Shannon J. Williamson (38225), Williamson, Shannon J., Cary, S. Craig, S. Craig Cary (133811), Craig W Herbold, Shannon J Williamson, McDonald, Ian R., Wommack, K. Eric   +20 more
core   +1 more source

Genotype/subgenotype distribution of hepatitis B virus among hemodialysis patients with chronical hepatitis B

Annals of Hepatology, 2012
Introduction. Hepatitis B virus (HBV) infection in hemodialysis (HD) patients is a major concern, but limited information exists on the HBV genotyping in patients on maintenance HD in Turkey.Aim.
Murat Sayan, M.D., Cengiz Dogan
doaj   +1 more source