Results 81 to 90 of about 2,958,232 (316)

Internally controlled real-time PCR method for quantitative species-specific detection and vapA genotyping of Rhodococcus equi [PDF]

, 2006
We developed a novel quantitative real-time PCR (Q-PCR) method for the soil actinomycete Rhodococcus equi, an important horse pathogen and emerging human pathogen.
Makrai, L, D. A. Lewis, Rodríguez-Lázaro, AD, Scortti, MM, Rodríguez-Lázaro, David, Hernandez, Marta, Lewis, DA, Vazquez-Boland, Jose A, M. Hernandez, J. A. Vazquez-Boland, Makrai, Laszlo, U. Fogarty, Scortti, Mariela, Hernández, M, Navas, Jesus, Ocampo-Sosa, A, Vázquez-Boland, JA, J. Navas, Ocampo-Sosa, Alain A, Lewis, Deborah A, Fogarty, U, Fogarty, Ursula, M. Scortti, A. A. Ocampo-Sosa, L. Makrai, Navas, J, D. Rodriguez-Lazaro   +26 more
core   +1 more source

IMPDH inhibition enhances cytarabine efficacy in SAMHD1‐expressing leukaemia cells via guanine nucleotide depletion

Molecular Oncology, EarlyView.
Cytarabine is a key therapy for acute myeloid leukaemia (AML), but its efficacy is limited by the dNTPase SAMHD1, which hydrolyses its active metabolite. Screening nucleotide biosynthesis inhibitors revealed that IMPDH inhibitors selectively sensitise SAMHD1‐proficient AML cells to cytarabine.
Miriam Yagüe‐Capilla, Christopher Dirks, Caroline Eiden, Sonja K. Fesenmayer, Femke M. Hormann, Yolande Klootsema, Ingrid Lilienthal, Si Min Zhang, Nikolas Herold, Sean G. Rudd   +9 more
wiley   +1 more source

Microvariation artifacts introduced by PCR and cloning of closely related 16S rRNA gene sequences [PDF]

, 2001
A defined template mixture of seven closely related 16S-rDNA clones was used in a PCR-cloning experiment to assess and track sources of artifactual sequence variation in 16S rDNA clone libraries. At least 14% of the recovered clones contained aberrations.
Speksnijder, Arjen, Jong, S., de, Kowalchuk, G.A., Stephen, J.R., De Jong, S., Speksnijder, A.G.C.L., Jong, S. de, Kline, E., Laanbroek, H.J.   +8 more
core   +3 more sources

PCR-based genotyping of Usp46 flox KI design.

, 2022
(a) PCR-RFLP design to validate LoxP KI alleles. The AscI and EcoRV digest the restriction sites adjacent to Left LoxP and Right LoxP, respectively. The arrows represent PCR primers for the digested DNA fragments, including PCR product sizes.
Michito Hamada (11965042), Masafumi Muratani (4760067), Natsumi Iki (11965030), Shinya Ayabe (3592004), Tra Thi Huong Dinh (11965039), Akihiro Kuno (4733382), Atsushi Yoshiki (102433), Kotaro Sakamoto (4019420), Sayaka R. Suzuki (11965018), Arata Wakimoto (11965024), Yoko Daitoku (11965036), Satoru Takahashi (32871), Seiya Mizuno (6169970), Natsuki Mikami (11965027), Kanako Kato (5145539), Yuko Hamada (142543), Megumi Takemura (11965033), Kazuya Murata (4043480), Yoshihisa Ikeda (564228), Miyuki Ishida (7829756), Fumihiro Sugiyama (759758), Yoko Tanimoto (6158447), Kento Morimoto (11965021)   +22 more
core   +1 more source

Asymptomatic Cryptosporidium infections in ewes and lambs are a source of environmental contamination with zoonotic genotypes of Cryptosporidium parvum

Parasite, 2020
Protozoan parasites of the Cryptosporidium genus cause severe cryptosporidiosis in newborn lambs. However, asymptomatic infections also occur frequently in lambs and ewes. In sheep, the most commonly detected Cryptosporidium species are C.
Bordes Léa, Houert Pauline, Costa Damien, Favennec Loïc, Vial-Novella Corinne, Fidelle Francis, Grisez Christelle, Prévot Françoise, Jacquiet Philippe, Razakandrainibe Romy   +9 more
doaj   +1 more source

KDM7A and KDM1A inhibition suppresses tumour promoting pathways in prostate cancer

Molecular Oncology, EarlyView.
Treatment resistance is a major challenge for patients with advanced prostate cancer. This study examined an alternative approach to target the major prostate cancer‐promoting pathway by targeting epigenetic factors, whose levels are higher in tumours.
Jennie N Jeyapalan, Veronika M Metzler, Simone de Brot, Corinne L Woodcock, Anna E Harris, Jennifer Lothion‐Roy, Emeli M Nilsson, Atara Ntekim, Michael S Toss, Jenny L Persson, Francesca Khani, Brian D Robinson, Lorraine J Gudas, Emad Rakha, David M Heery, Catrin S Rutland, Nigel P Mongan   +16 more
wiley   +1 more source

A direkt sejtes PCR technika tesztelése laborunkban

, 2015
A direkt sejtes PCR technika egy gyors és költséghatékony módszer bizonyos nukleinsav szakaszok amplifikálására. A DNS izolálás hosszadalmas lépéseit kihagyva lehet közvetlenül, spórából vagy hifából templátot készíteni a PCR reakcióhoz.gjBiomérnökiBSc ...
Tamasik, Dániel
core  

Development of a temperature-switch PCR-based SNP typing method for Mycobacterium ulcerans [PDF]

, 2012
Mycobacterium ulcerans (M. ulcerans), the causative agent of the devastating skin disease Buruli ulcer (BU), is characterized by an extremely low level of genetic diversity.
Danso Emelia, Röltgen, K., Gerd Pluschke, Pluschke Gerd, Yeboah-Manu, D., Katharina Röltgen, Dorothy Yeboah-Manu (120059), Gerd Pluschke (7855), Emelia Danso, Katharina Röltgen (120045), Danso, E., Pluschke, G., Kobina Assan-Ampah, Emelia Danso (120054), Dorothy Yeboah-Manu, Kobina Assan-Ampah (120049), Yeboah-Manu Dorothy, Assan-Ampah Kobina, Assan-Ampah, K., Röltgen Katharina   +19 more
core   +1 more source

Flexible methods for uncertainty estimation of digital PCR data

iScience
Summary: Digital PCR (dPCR) is an accurate technique for quantifying nucleic acids, but variance estimation remains a challenge due to violations of the assumptions underlying many existing methods.
Yao Chen, Ward De Spiegelaere, Matthijs Vynck, Wim Trypsteen, David Gleerup, Jo Vandesompele, Olivier Thas   +6 more
doaj   +1 more source

Transcriptional profiling of circulating extracellular vesicles from prebiopsy prostate cancer patients

Molecular Oncology, EarlyView.
RNA profiling of circulating extracellular vesicles (EVs) from blood samples of men undergoing prostate biopsy identifies transcripts associated with clinically significant prostate cancer. Integrative analysis with public tumor datasets links EV‐derived gene signatures to tumor stage and progression‐free survival, highlighting CASP3, XRCC2, and RIT1 ...
Stefan Werner, Pierre Tennstedt, Randi C. Pose, Christian Müller, Katharina Besler, Svenja Schneegans, Malik Alawi, Marie C. Roesch, Sven Peine, Desiree Bonci, Joanna Budna‐Tukan, Evi Lianidou, Catherine Alix‐Panabières, Derya Tilki, Klaus Pantel   +14 more
wiley   +1 more source