Results 71 to 80 of about 2,958,232 (316)

The Shewanella oneidensis Fic enzyme SoFic targets the switch‐I region of EF‐Tu for AMPylation

FEBS Letters, EarlyView.
Fic enzymes mediate diverse post‐translational modifications across all domains of life, including AMPylation. Prokaryotic EF‐Tu can be AMPylated and deAMPylated by the conserved Fic enzyme SoFic. Structural and biochemical approaches were used to characterize the effect of AMPylation on EF‐Tu, SoFic's enzymatic activities, and the enzyme‐target ...
Svenja Runge, Vivian Pogenberg, Alexander Baumgart, Bente Siebels, Hartmut Schlüter, Michael Hecht‐Bucher, Aymelt Itzen   +6 more
wiley   +1 more source

A novel real-time PCR for Listeria monocytogenes that monitors analytical performance via an internal amplification control [PDF]

, 2005
We describe a novel quantitative real-time (Q)-PCR assay for Listeria monocytogenes based on the coamplification of a target hly gene fragment and an internal amplification control (IAC). The IAC is a chimeric double-stranded DNA containing a fragment of
Vázquez-Boland, JA, Pla, Maria, Monzó, HJ, Rodríguez-Lázaro, AD, Scortti, MM, Rodríguez-Lázaro, David, Monzó, Hector J, Pla, M, Scortti, Mariela, Vazquez-Boland, Jose A   +9 more
core  

L‐aspartate oxidase provides new insights into fumarate reduction in anaerobic darkness in Synechocystis sp. PCC6803

FEBS Letters, EarlyView.
Synechocystis strains deficient in succinate dehydrogenase (SDH) secrete more succinate than the WT under dark anaerobic conditions, supporting that SDH then primarily acts as SDH, not as a fumarate reductase. L‐aspartate oxidase (Laspo) from Synechocystis is functional under anaerobic conditions, reducing fumarate to succinate.
Kateryna Kukil, Jeffrey A. Hawkes, Cecilia Blikstad, Pia Lindberg   +3 more
wiley   +1 more source

PCR-confirmed cases.

, 2019
PCR-confirmed cases.
Rebecca Bridge (8156520), Michael Worobey (31168), James O. Lloyd-Smith (8156526), Shane Brady (8156523), Katelyn M. Gostic (6885455), Cécile Viboud (27823)   +5 more
core   +1 more source

A context‐dependent modulatory role for eIF6 in acquired resistance to vemurafenib in melanoma

FEBS Letters, EarlyView.
Acquired resistance to vemurafenib upregulates the translation factor eIF6 in melanoma cells. Silencing eIF6 in resistant cells reduces proliferation and partially restores drug sensitivity, whereas its overexpression increases sensitivity across melanoma lines regardless of BRAF status, via modulation of mTOR, S6K, and MAPK signaling.
George Kyriakopoulos, Antonia Petropoulou, Alexandra Anastogianni, Angelina Bania, Vassilis Stamatakis, Argyris Alexiou, Chi‐Ping Day, Glenn Merlino, Katerina Grafanaki, Constantinos Stathopoulos   +9 more
wiley   +1 more source

Comparison of quantitative real time PCR with Sequencing and ribosomal RNA-FISH for the identification of fungi in Formalin fixed, paraffin-embedded tissue specimens [PDF]

, 2011
Background: Identification of the causative agents of invasive fungal infections (IFI) is critical for guiding antifungal therapy. Cultures remain negative in a substantial number of IFI cases.
Rickerts Volker, Ko Daisy L, Fredricks, David N., Prasanna D Khot, David N Fredricks, David Myerson, Khot Prasanna D, Myerson David, Ko, Daisy L., Khot, Prasanna D., Myerson, David, Volker Rickerts, Lambrecht, Evelyn, Lambrecht Evelyn, Rickerts, Volker, Fredricks David N, Daisy L Ko, Evelyn Lambrecht   +17 more
core   +2 more sources

PCR-RFLP.

, 2022
Restriction fragment length polymorphism analysis of ITS1-PCR fragments amplified from DNA samples using Hae III enzyme. NC: Negative control (water); M: molecular marker (123 bp); La: Leishmania amazonensis (IOC / L0575-MHOM / BR / 1967 / PH8); Lb ...
Gabriela Torres Rebech (6053297), Valéria Marçal Felix de Lima (8115269), Sidnei Ferro Costa (8115260), Flavia de Rezende Eugênio (12290056), Marilene Oliveira dos Santos (12290053), Jaqueline Poleto Bragato (5488034), Jéssica Henrique de Freitas (12290050), Paulo Sérgio Patto dos Santos (12290059)   +7 more
core   +1 more source

Reassessment of Aeromonas oralensis. Comment on Mashzhan et al. Whole-Genome Sequencing of a Potentially Novel Aeromonas Species Isolated from Diseased Siberian Sturgeon (Acipenser baerii) Using Oxford Nanopore Sequencing. Microorganisms 2025, 13, 1680

Microorganisms
In this comment, we re-evaluate the proposal of Aeromonas oralensis as a novel species by Mashzhan et al. (2025) [...]
Antonio Martínez-Murcia, Aaron Navarro, Caridad Miró-Pina   +2 more
doaj   +1 more source

The role of miR‐335‐5p in the redifferentiation of BRAF p.V600E thyroid cancers

Molecular Oncology, EarlyView.
The BRAF p.V600E mutation promotes thyroid cancer dedifferentiation and radioiodine resistance. Using a network approach, we identified miR‐335‐5p as a key regulator of BRAF‐mutated thyroid tumors. Restoring miR‐335‐5p increased thyroid‐specific gene expression and iodine uptake in cells and organoids.
Valeria Pecce, Simone Bini, Marialuisa Sponziello, Giorgio Grani, Giulia Fiscon, Paola Paci, Lorenzo Farina, Rosa Falcone, Valentina Maggisano, Sebastiano Filetti, Cosimo Durante, Antonella Verrienti   +11 more
wiley   +1 more source

A PCR sikertörténete

, 2012
A polimeráz láncreakciót ki és hogyan fedezte fel. A módszer alkalmazása DNS-szekvenálásra.
Czinege, Anikó
core