Improved PCR-DGGE analysis by emulsion-PCR for the determination of food geographical origin: A case study on Greek PDO “avgotaracho Mesolonghiou” [PDF]
Current Research in Food Science, 2021 Greek avgotaracho Mesolonghiou (fish eggs from Flathead Mullet) is a highly valuable food product which holds Protected Destination of Origin status. The aim of this work was to use PCR-DGGE technique to examine whether there is a correlation between ...
Maria-Eleni Dimitrakopoulou +2 more
doaj +2 more sources
Comparison of culture and PCR-DGGE methods to evaluate the airways of cystic fibrosis patients and determination of their antibiotic resistance profile [PDF]
Iranian Journal of Microbiology, 2023 Background and Objectives: Respiratory infections are the most serious condition in cystic fibrosis (CF) patients; therefore, a thorough comprehension of the diversity and dominant microbial species in CF airways has a crucial role in treatment.
Somayeh Moazami Goudarzi +7 more
doaj +2 more sources
Homing in on Endogenous Badnaviral Elements: Development of Multiplex PCR-DGGE for Detection and Rapid Identification of Badnavirus Sequences in Yam Germplasm [PDF]
Frontiers in Plant Science, 2022 Viruses of the genus Badnavirus (family Caulimoviridae) are double-stranded DNA-reverse transcribing (dsDNA-RT) plant viruses and have emerged as serious pathogens of tropical and temperate crops globally.
Gonçalo Silva +7 more
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Bacterial and Fungal Communities of Gioddu as Revealed by PCR-DGGE Analysis. [PDF]
Indian J Microbiol, 2020 Gioddu is the sole variety of fermented milk originating in Italy. Despite the long history of consumption, Gioddu still represents an undisclosed source of microbial diversity. The present study was aimed to get an insight into the bacterial and fungal diversity of Gioddu samples collected from two artisan producers located in Sardinia.
Maoloni A +8 more
europepmc +4 more sources
Approach to determine the diversity of Legionella species by nested PCR-DGGE in aquatic environments. [PDF]
PLoS ONE, 2017 In this study, we describe a nested PCR-DGGE strategy to detect Legionella communities from river water samples. The nearly full-length 16S rRNA gene was amplified using bacterial primer in the first step.
Wen-Chien Huang +7 more
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Archives of Environmental Protection, 2014 Nitritation, the first stage of ammonia removal process is known to be limiting for total process performance. Ammonia oxidizing bacteria (AOB) which perform this process are obligatory activated sludge habitants, a mixture consisting of Bacteria ...
Ziembińska-Buczyńska Aleksandra +2 more
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Emulsion PCR (ePCR) as a Tool to Improve the Power of DGGE Analysis for Microbial Population Studies
Microorganisms, 2020 To the authors’ knowledge, this is the first report of the use of emulsion-Polymerase chain reaction (e-PCR) coupled with denaturing gradient gel electrophoresis (DGGE) analysis. In the present work the effectiveness of ePCR in improving the power of the
Lucilla Iacumin +3 more
doaj +3 more sources
Analysis of microbial diversity in Shenqu with different fermentation times by PCR-DGGE. [PDF]
Braz J Microbiol, 2017 Shenqu is a fermented product that is widely used in traditional Chinese medicine (TCM) to treat indigestion; however, the microbial strains in the fermentation process are still unknown. The aim of this study was to investigate microbial diversity in Shenqu using different fermentation time periods.
Liu T +5 more
europepmc +5 more sources
Comparison of DNA extraction kits for PCR-DGGE analysis of human intestinal microbial communities from fecal specimens [PDF]
Nutrition Journal, 2010 Background The influence of diet on intestinal microflora has been investigated mainly using conventional microbiological approaches. Although these studies have advanced knowledge on human intestinal microflora, it is imperative that new methods are ...
Nakatsu Cindy H +2 more
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Impact of whole grain highland hull-less barley on the denaturing gradient gel electrophoresis profiles of gut microbial communities in rats fed high-fat diets [PDF]
Microbiology SpectrumPolymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) is a traditional non-culture technique that can provide a fingerprint of the microbial community.
Xuejuan Xia +6 more
doaj +2 more sources