Results 21 to 30 of about 769,066 (216)
Application of Denaturing Gradient Gel ElectrophoresisApplication of Denaturing Gradient Gel Electrophoresis(DGGE) Methods on Parent-Offspring Relationship of the Coral Pocillopora damicornis [PDF]
, 2008 DGGE (Denaturing Gradient Gel Electrophoresis) is the most powerful methods for mutation detection currentlyavailable. In DGGE, DNA fragments of the same length but with different sequences can be separated.
Permata W, Diah, Hirose, M, Hidaka , M
core +1 more source
International Journal of Speleology, 2014 The aim of this study was to experimentally verify the significance of microbial transport through low-permeability fault zones in a compartmentalised carbonate aquifer system in Southern Italy.
Antonio Bucci +4 more
doaj +1 more source
Lactic acid bacteria and identification with PCR-DGGE
International Journal of Biological Research, 2017 Lactic acid bacteria (LAB) are an important group in the industrially using microorganisms. The first pure cultures of bacteria was "Bacterium lactis" (probably Lactococcus lactis), obtained in 1873 by J. Lister. LAB are Gram-positive, non motile, non spore-forming, except Sporolactobacillus inulinus, catalase negative, microaerophilic or anaerobic ...
Yusuf BİÇER, Gürkan UÇAR
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Analysis of Microbial Diversity in Nuruk Using PCR-DGGE [PDF]
Journal of Life Science, 2012 Nuruk plays a significant role in the flavor and quality of Takju and Yakju, which are produced through saccharification and alcohol fermentation by various microorganisms. In this study, we identified microbial strains isolated from a plate count and PCR-denaturing gradient gel electrophoresis (DGGE) analysis targeting the 16S and 28S rRNA genes, in ...
Seung-Jik Kwon, Jae-Hak Sohn
openaire +1 more source
Exon Concatenation to Increase the Efficiency of Mutation Screening by DGGE
BioTechniques, 2002 For genes that have a substantial number of exons and long intronic sequences, mutation screening by denaturing gradient gel electrophoresis (DGGE) requires the amplification of each exon from genomic DNA by PCR.
X. Agirre +5 more
doaj +1 more source
Development of a DGGE method to explore Legionella communities
Heliyon, 2020 Legionella risk assessment is nowadays based on the presence and concentration of either Legionella pneumophila or Legionella spp. Many species of Legionella can cause Legionnaires’ disease, indeed about half of the known species have been associated ...
S. Bayle +6 more
doaj +1 more source
Molecular analysis of microbial communities in endotracheal tube biofilms. [PDF]
PLoS ONE, 2011 BACKGROUND: Ventilator-associated pneumonia is the most prevalent acquired infection of patients on intensive care units and is associated with considerable morbidity and mortality.
Scott Cairns +7 more
doaj +1 more source
Autogenic versus environmental control during development of river biofilm [PDF]
, 2009 In the natural environment, microbial community structure of river biofilm is controlled by biotic and abiotic factors. This study explored the capacity to manipulate the structure of microbial communities by modifying environmental conditions during the
Émilie Lyautey +7 more
core +1 more source
Journal of Lipid Research, 1995 Familial hypercholesterolemia (FH) results from mutations in the low density lipoprotein receptor (LDLR) gene. We applied denaturing gradient gel electrophoresis (DGGE) to screen for sequence variations in the coding and splice site consensus sequences ...
P Lombardi +6 more
doaj +1 more source
Assessment of variation in bacterial composition among microhabitats in a mangrove environment using DGGE fingerprints and barcoded pyrosequencing. [PDF]
PLoS ONE, 2012 Here, we use DGGE fingerprinting and barcoded pyrosequencing data, at six cut-off levels (85-100%), of all bacteria, Alphaproteobacteria and Betaproteobacteria to assess composition in the rhizosphere of nursery plants and nursery-raised transplants ...
Daniel F R Cleary +3 more
doaj +1 more source