Study of the bacterial diversity of foods: PCR-DGGE versus LH-PCR
The present study compared two culture-independent methods, polymerase chain reaction denaturing gradient gel electrophoresis (PCR-DGGE) and length-heterogeneity polymerase chain reaction (LH-PCR), for their ability to reveal food bacterial microbiota.
Cristiana Garofalo +2 more
exaly +4 more sources
Safety evaluation and bacterial community of kung-som using PCR-DGGE technique [PDF]
This study evaluates the safety of kung-som which was distributed in local markets and using PCR-DGGE technique to identify microflora in kung-som. Lactic acid bacteria (LAB) were found at counts of more than 7 log CFU g-1 in all samples and the total ...
Sutanate Saelao +3 more
doaj +2 more sources
Analysis on Microbial Flora Changes During Processing and Storage of Spiced Goose Based on Pcr-Dgge Combined with Conventional Microbial Culture Methods [PDF]
During the processing and storage process of spiced goose, samples were collected from raw goose meat (R0), spiced goose meat cooled for 2 hours (2h) and spiced goose meat stored for 1day (1d), 3 days (3d) and 5 days (5d).
Li Xing +7 more
doaj +1 more source
Approach to analyze the diversity of myxobacteria in soil by semi-nested PCR-denaturing gradient gel electrophoresis (DGGE) based on taxon-specific gene. [PDF]
The genotypic diversity of insoluble macromolecules degraded myxobacteria, provided an opportunity to discover new bacterial resources and find new ecological functions.
Baiyuan Li, Qing Yao, Honghui Zhu
doaj +1 more source
Evaluating primers for profiling anaerobic ammonia oxidizing bacteria within freshwater environments. [PDF]
Anaerobic ammonia oxidizing (anammox) bacteria play an important role in transforming ammonium to nitrogen gas and contribute to fixed nitrogen losses in freshwater environments.
Puntipar Sonthiphand, Josh D Neufeld
doaj +1 more source
بررسی تکنیک PCR-DGGE برای شناسایی گونههای مختلف کاندیدا [PDF]
کاندیدا از جمله شایعترین مخمرهای عفونت زای انسانی میباشد و در سالهای اخیر نرخ عفونتهای ایجاد شده توسط این مخمر افزایش یافته است. شناسایی زود هنگام کاندیداهای بیماری زا نقش مهمی را برای انجام اقدامات درمانی و کنترل عفونتهای بیمارستانی ایفا مینماید ...
آیدا حمیدخانی +3 more
doaj +1 more source
Diversity of Marine Microbes by PCR-DGGE
Recently, the development of various culture-independent identification techniques for environmental microbes has greatly enhanced our knowledge of microbial diversity. In particular, denaturing gradient gel electrophoresis (DGGE) of 16S rDNA fragments, generated using the polymerase chain reaction (PCR) is frequently used to examine the diversity of ...
Yeong-Jin Kim +5 more
openaire +2 more sources
Are Bifidobacterium Species Key Players in the Progression of Type 1 Diabetes? A Systematic Review. [PDF]
Bifidobacterium are generally considered beneficial bacteria, and are found in probiotic formulations currently being trialled for type 1 diabetes (T1D) prevention. In this systematic review, we find that there is a reduction in Bifidobacterium species after T1D diagnosis and an increase or no change in Bifidobacterium abundance in those at risk of T1D.
Vergoz V, Jeong D, Hamilton-Williams EE.
europepmc +2 more sources
Background: Marcha is an ethnic amylolytic starter that is used to ferment boiled cereals to produce alcoholic drinks, commonly called jaanr, in the Himalayan Regions of Sikkim and Darjeeling of India.
Shankar P. Sha +4 more
doaj +1 more source
Detection of Listeria pathogens by gradient/constant denaturing gel electrophoresis [PDF]
Listeriosis is a serious, but preventable disease, and the virulence factor of this disease is produced only by the two pathogenic species L. monocytogenes and L. ivanovii in humans and/or animals.
A. Al-Mariri, L. Ramadan, L. Al-Halab
doaj +1 more source

