Results 71 to 80 of about 1,217,363 (294)

PCR-RFLP detection of point mutations A2143G and A2142G in 23S rRNA gene conferring resistance to clarithromycin in Helicobacter pylori strains.

Acta Biochimica Polonica, 2014
BACKGROUND The occurrence of clarithromycin resistance among Helicobacter pylori strains is a major cause of the treatment failure. Resistance to this drug is conferred by point mutations in 23S rRNA gene and the most prevalent mutations are A2143G and ...
Karolina Klesiewicz, P. Nowak, E. Karczewska, I. Skiba, Izabela Wojtas-Bonior, E. Sito, A. Budak   +6 more
semanticscholar   +1 more source

‘Candidatus Phytoplasma solani’: From infection to all‐round characterisation of distinct tuf‐type strains with different virulence in tomato plants

Annals of Applied Biology, EarlyView.
‘Candidatus Phytoplasma (Ca. P.) solani’ is associated with bois noir (BN) of grapevine and stolbur of solanaceous plants and is primarily transmitted by Hyalesthes obsoletus Signoret. Four tuf‐a and five tuf‐b1 ‘Ca. P. solani’ strains were transmitted to tomato plants (cv. Micro‐Tom) by using bait‐plants and by capturing H.
Gaia Carminati, Francesco Pavan, Chiara Bernardini, Diana Sommaro, Abderraouf Sadallah, Rita Musetti, Paolo Ermacora, Marta Martini   +7 more
wiley   +1 more source

MITOCHONDRIAL DNA POLYMORPHISMS AND FERTILITY IN BEEF CATTLE [PDF]

, 2002
Two regions of mitochondrial DNA, D-loop and ND-5 were characterized using polymerase chain reaction – restriction fragment length polymorphism (PCR-RFLP) involving 422 beef cattle of Hereford and composite breeds from Wokalup’s ...
., Sutarno, A.J, Lymbery, J.M, Cummins, RCA, Thompson   +3 more
core  

Real-time PCR/MCA assay using fluorescence resonance energy transfer for the genotyping of resistance related DHPS-540 mutations in Plasmodium falciparum [PDF]

, 2008
BACKGROUND: Sulphadoxine-pyrimethamine has been abandoned as first- or second-line treatment by most African malaria endemic countries in favour of artemisinin-based combination treatments, but the drug is still used as intermittent preventive treatment ...
AO Talisuna, AP Alker, C Wongsrichanalai, CH Sibley, Chantal van Overmeir, CV Plowe, DG Altman, FO ter Kuile, G Dorsey, J Chaparro, J Le Bras, Jean-Claude Dujardin, JJ Juliano, Maryline Bonnet, MK Laufer, MT Duraisingh, P Ouma, PE Wilson, Petra F Mens, RA Cardullo, S Cohuet, S Decuypere, T Triglia, Umberto d'Alessandro, World Health Organization Regional Office for Africa, World Health Organization Regional Office for Africa   +25 more
core   +9 more sources

Genotoxicity evaluation of acephate and profenofos by the PCR-RFLP assay

Toxicology International, 2014
In this study we have evaluated the genotoxic potential of pesticides acephate and profenofos by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay with the mosquito Culex quinquefasciatus taken as experimental model.Second instar larvae were treated with LC20 of each pesticide for 24 h and induced mutations in the ...
Preety Bhinder, Asha Chaudhry
openaire   +4 more sources

Fly diversity revealed by PCR‐RFLP of mitochondrial DNA [PDF]

Biochemistry and Molecular Biology Education, 2008
AbstractIn this article, we describe an inexpensive, two‐session undergraduate laboratory activity that introduces important molecular biology methods in the context of biodiversity. In the first session, students bring tentatively identified flies (order Diptera, true flies) to the laboratory, extract DNA, and amplify a region of the mitochondrial ...
Nancy P. Sayar, Colin A. Smith, Khouzama M. Knio, Jimmy F. Asraoui   +3 more
openaire   +3 more sources

Coconut rhinoceros beetle Oryctes rhinoceros (Coleoptera: Scarabaeidae) confirmed in the Americas

EPPO Bulletin, EarlyView.
Abstract In 2022, a suspected incursion of Oryctes rhinoceros (coconut rhinoceros beetle, CRB) was reported in Bahía de Banderas, Nayarit, México, based on damage symptoms observed on coconut palms and photographs of beetles on a social media site. This prompted collaboration with local biologists and land managers, and a survey of the area with the ...
Trevor A. Jackson, Miguel Nájera‐Rincón, Sulav Paudel, Mitchell K. Weston, Amy Beattie, Sean D. G. Marshall   +5 more
wiley   +1 more source

Genetic diversity among Frankia strains nodulating members of the family Casuarinaceae in Australia revealed by PCR and restriction fragment length polymorphism analysis with crushed root nodules [PDF]

, 1996
DNA extracted directly from nodules was used to assess the genetic diversity of #Frankia# strains symbiotically associated with two species of genus #Casuarina# and two of the genus #Allocasuarina# naturally occuring in northeastern Australia.
Normand, Philippe, Prin, Yves, Reddell, Paul, Rouvier, Carole, Simonet, Pascal   +4 more
core  

Characterization of two new alleles at the goat CSN1S2 locus. [PDF]

, 2001
Two novel alleles at the goat CSN1S2 locus have been identified: CSN1S2(F) and CSN1S2(D). Sequence analyses revealed that the CSN1S2(F) allele is characterized by a G --> A transition at the 13th nucleotide in exon 3 changing the seventh amino acid of ...
COSENZA, GIANFRANCO, DI GREGORIO P, GALLO, DANIELA, LONGOBARDI E, PAPPALARDO, MARGHERITA, PASTORE N, RAMUNNO, LUIGI, RANDO A.   +7 more
core   +1 more source

Molecular Testing in Sickle Cell Disease: From Newborn Screening to Transfusion Care

International Journal of Laboratory Hematology, EarlyView.
ABSTRACT Sickle cell disease (SCD) is one of the most frequent monogenic diseases worldwide and a highly heterogeneous and complex disease. SCD care carries several challenges. This includes early and accurate diagnosis as well as optimal red blood cell transfusion matching in this population carrying a high risk of alloimmunization.
Thomas Pincez, Yves D. Pastore
wiley   +1 more source