Results 81 to 90 of about 811,842 (227)

Application of polymerase chain reaction (PCR) and PCR based restriction fragment length polymorphism for detection and identification of dermatophytes from dermatological specimens

Indian Journal of Dermatology, 2008
Objective: To develop and optimize polymerase chain reaction-based restriction fragment length polymorphism (PCR-RFLP) targeting 18S rDNA and internal transcribed spacer (ITS) region of fungi for rapid detection and identification of dermatophytes ...
Bagyalakshmi R, Senthilvelan B, Therese K, Murugusundram S, Madhavan H   +4 more
doaj  

Rapid PCR-RFLP method for identification of five billfish species [PDF]

, 2010
[[abstract]]A rapid and reliable polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method was developed to identify billfish species Xiphias gladius (swordfish), Makaira nigricans (blue marlin), Makaira indica (black marlin ...
Hung-Sheng Hsieh;Tuu-Jyi Chai;Deng-Fwu Hwang
core  

Primers and PCR programs for HVEM PCR-RFLP genotyping.

, 2013
Primers and PCR programs for HVEM PCR-RFLP genotyping.
Dalin Li (91843), Dianjun Li (124542), Liqun Li (446845), Zhenkun Fu (124534), Yanhong Liu (124527), Shuang Chen (12981), Weiguang Yuan (124539), Da Pang (110728)   +7 more
core   +1 more source

PCR-RFLP for Campylobacter jejuni subtyping [PDF]

, 2020
Diez cepas de Campylobacter jejuni aisladas de fetos porcinos abortados fueron identificadas por pruebas bioquímicas: 8 como C. jejuni biotipo II de Lior, y 2 como C. jejuni biotipo I.
Perfumo, Carlos Juan, Echeverría, María Gabriela, Giacoboni, Gabriela   +2 more
core  

Resistance of Botrytis cinerea to anilinopyrimidine fungicides: A novel ARMS‐PCR method for the detection of Bcpos5 mutations and characterization of resistance using CRISPR/Cas9 editing

Pest Management Science, Volume 82, Issue 9, Page 8189-8198, September 2026.
A TETRA‐primer amplification refractory mutation system polymerase chain reaction (T‐ARMS‐PCR) method was developed for simultaneous detection of the L412F/V and G408V mutations. L412F/V mutants were created using the CRISPR/Cas tool and no fitness cost was observed.
Georgios Sofianos, Athanasios Petmezas, Anastasios Samaras, George Karaoglanidis   +3 more
wiley   +1 more source

Human Cytomegalovirus Genetic Diversity, Clinical Relevance, and Emerging Insights

Reviews in Medical Virology, Volume 36, Issue 5, September 2026.
ABSTRACT Human cytomegalovirus (HCMV) is a globally prevalent virus that poses a significant public health concern, especially for newborns and immunocompromised patients, causing a wide range of infections. Clinical outcomes associated with HCMV infection include congenital disease, graft rejection in transplant recipients, and life‐threatening ...
Hajar Y. AlQahtani, Fadilah Sfouq Aleanizy, Fulwah Yahya Alqahtani   +2 more
wiley   +1 more source

Genetic variability of myostatin and prolactin genes in popular goat breeds in Egypt

Journal of Genetic Engineering and Biotechnology, 2018
The genetic polymorphisms of two functional genes named: myostatin (MSTN) and prolactin (PRL) were investigated in three goat breeds (Barki, Damascus and Zaraibi) using Sanger nucleotide sequence and restriction fragment length polymorphism (RFLP ...
Sekena H. Abdel-Aziem, K.F. Mahrous, M.A.M. Abd El-Hafez, M. Abdel Mordy   +3 more
doaj   +1 more source

Sequence differences in the diagnostic target region of the oocyst wall protein gene of Cryptosporidium parasites [PDF]

, 2000
Nucleotide sequences of the Cryptosporidium oocyst wall protein (COWP) gene were obtained from Various Cryptosporidium spp. (C. wrairi, C. felis, C. meleagridis, C. baileyi, C. andersoni, C. muris, and C. serpentis) and C. parvum genotypes (human, bovine,
Sulaiman, I., Limor, J., Thompson, R.C.A., Morgan, U.M., Xiao, L., Lal, A.A.   +5 more
core  

Transcriptional and Functional Insights Into the All‐Stage Resistance Gene GmRmd1‐Mediated Regulation of Resistance to Powdery Mildew in Soybean

Molecular Plant Pathology, Volume 27, Issue 9, September 2026.
GmRmd1 is a soybean all‐stage powdery mildew (PMD) resistance gene. Under normal conditions, GmWRKY27 and GmERF138 suppress the expression of GmRmd1. Upon Microsphaera diffusa infection, GmRmd1 interacts with GmRmd2, GmRmd3, GmRnl1 and GmPER1, forming functional protein complexes to regulate lignin biosynthesis and thereby modulate disease resistance ...
Guoqiang Liu, Chen Ling, Tingting Xu, Xuehui Zhang, Jiacan Jiang, Xiaomin Xu, Lu Li, Jiajian Zhang, Bing Hu, Mengshi Liu, Yunxin He, Cunyi Yang   +11 more
wiley   +1 more source

Restriction fragment length polymorphism analysis of isolates of infectious bursal disease viruses from Turkey

Veterinární Medicína, 2003
Infectious bursal disease Virus (IBDV) specific reverse transcriptase/polymerase chain recation (RT/PCR) positive 40 broiler bursa fabricius samples obtained from a commercially reared flock were investigated for genetic diversity by PCR-RFLP assay.
G. Ozbes, H.B. Ertas, A. Muzo
doaj   +1 more source