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PCR-RFLP pattern of three kinds of Metagonimus in Korea
We tried to compare the three kinds of Metagonimus species, M. yokogawai, Metagonimus Miyata type, and M. takahashii, which were known to be distributed in Korea with polymerase chain reaction based-restriction fragment length polymorphism (PCR-RFLP) patterns. We amplified the internal transcribed spacer 1 (ITS1) site of ribosomal RNA and mitochondrial
J R, Yu +4 more
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(A) PCR-RFLP analysis of NPY exon 2 (+1128; T/C) polymorphism on 2.0% agarose gel: lanes: 1 and 5 show homozygous (TT) genotypes; lane: 2 and 6 show heterozygous (TC) genotypes; lane: 3 shows homozygous (CC) genotype; lane: 4 shows 100 bp DNA ladder. (B)
Roma Patel (3224778) +7 more
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Detección de Bartonella bacilliformis usando PCR-RFLP
Reportamos la aplicación de las técnicas moleculares PCR-RFLP para la confirmación de infecciones por Bartonella bacilliformis. El método de PCR-RFLP se basa en la amplificación in vitro de un fragmento de 380 pb correspondiente al gen citrato sintetasa ...
C Padilla +4 more
doaj
COMPARISON OF 16S rRNA-PCR-RFLP, LipL32-PCR AND OmpL1-PCR METHODS IN THE DIAGNOSIS OF LEPTOSPIROSIS
SUMMARY Leptospirosis is still one of the most important health problems in developing countries located in humid tropical and subtropical regions. Human infections are generally caused by exposure to water, soil or food contaminated with the urine of ...
Cansu ÖNLEN +4 more
core +2 more sources
The Comprehensive Exploration of PCR-RFLP
This comprehensive review navigates the intricate landscape of Polymerase Chain Reaction – Restriction Fragment Length Polymorphism (PCR-RFLP), providing an in-depth exploration of its processes, applications, advantages, drawbacks, and future potential.
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A preliminary study using multi-target polymerase chain reaction (multiplex PCR) and restriction fragment length polymorphism (PCR-RFLP) was done on the same feedstuffs to detect animal tissues.
TREZZI, ISABELLA ELENA +6 more
core +1 more source
Hepatitis B virus (HBV) can be classified into 8 genotypes, genotype A to H. Genotype of HBV is important for clinical and etiological investigations. Research for HBV genotyping, HBV transmission study using nested PCR and HBV genotyping based on RFLP ...
Aris Haryanto +4 more
doaj
[Echinococcus granulosus genotyping by PCR-RFLP].
A method to assess the length polymorphism of restriction fragments of polymerase chain reaction (PCR-LPRF) has been developed and used for the genomic typing of E. granulosus. A scheme optimal under our conditions has been chosen for analysis. To test the chosen scheme, 10 E.
N A, Nikulina +2 more
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Identification of Escherichia coli requires knowledge regarding the prevalent serotypes and virulence factors profiles allows the classification in pathogenic/non-pathogenic. However, some of these bacteria do not express flagellar antigen invitro.
Cláudia de Moura +3 more
doaj
The hsp70 PCR-RFLP assay described by Graça et al. (2012) enables to identifify different species of the genus Leishmania. Graça et.al. (2012) described four different primer pairs and associated with some restriction enzymes for the identification of Leishmania species.The hsp70 PCR-RFLP assay using the hsp70 C primers described by Graça et al. (2012)
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