Results 71 to 80 of about 861,394 (224)

Human papillomavirus detection and typing using a nested-PCR-RFLP assay

Brazilian Journal of Infectious Diseases
BACKGROUND: It is clinically important to detect and type human papillomavirus (HPV) in a sensitive and specific manner. OBJECTIVES: Development of a nested-polymerase chain reaction-restriction fragment length polymorphism (nested-PCR-RFLP) assay to ...
Janaina Coser, Thaís da Rocha Boeira, André Salvador Kazantzi Fonseca, Nilo Ikuta, Vagner Ricardo Lunge   +4 more
doaj   +1 more source

Genotyping of Flavobacterium psychrophilum using PCR-RFLP analysis [PDF]

Diseases of Aquatic Organisms, 2003
Genetic variability among 242 strains of Flavobacterium psychrophilum was characterized using polymerase chain reaction (PCR) followed by restriction fragment length polymorphism (RFLP) analysis. Universal Primers GYR-1 and GYR-1R, which were designed to amplify the gyrase subunit B gene (gyrB), yielded a 1178 bp PCR product encoding gyrB and a 290 bp ...
Shotaro, Izumi, Futoshi, Aranishi, Hisatsugu, Wakabayashi   +2 more
openaire   +2 more sources

LAMP Assay for Detection and Differentiation of ‘Candidatus Phytoplasma solani’ 16SrXII‐P and 16SrXII‐A in Beta vulgaris and Pentastiridius leporinus Using Crude Extracts

Plant Pathology, Volume 75, Issue 6, November‐December 2026.
A rapid LAMP assay targeting the stamp2 gene enables specific detection of ‘Candidatus Phytoplasma solani’ 16SrXII‐P. Combined with an existing assay, it allows fast and reliable discrimination of two major subgroups in sugar beet and insect vectors within 30 min using simple crude extracts.
Dennis Rahenbrock, Tjaša Jakomin, Mark Varrelmann, Omid Eini, Nataša Mehle   +4 more
wiley   +1 more source

Online exercise for the design and simulation of PCR and PCR-RFLP experiments [PDF]

, 2013
[EN] Background: Polymerase Chain Reaction (PCR) and Restriction Fragment Length Polymorphism of PCR products (PCR-RFLP) are extensively used molecular biology techniques.
Javier Garaizar, San Millán Gutiérrez, Rosario María, Rementeria Ruiz, Aitor Domingo, Joseba Bikandi, Martínez Ballesteros, Ilargi, Bikandi Bikandi, Joseba, Garaizar Candina, Javier, Rosario San Millán, Aitor Rementeria, Ilargi Martínez-Ballesteros   +9 more
core   +1 more source

Blood Meal Identification in Field-Captured Sand flies: Comparison of PCR-RFLP and ELISA Assays

Journal of Arthropod-Borne Diseases, 2009
Background: We aimed to develop a PCR-RFLP assay based on available sequences of putative vertebrate hosts to iden­tify blood meals ingested by field female sand fly in the northwest of Iran.
N Maleki-Ravasan, MA Oshaghi, E Javadian, Y Rassi, J Sadraei, F Mohtarami   +5 more
doaj  

Genotoxicity evaluation of acephate and profenofos by the PCR-RFLP assay

Toxicology International, 2014
In this study we have evaluated the genotoxic potential of pesticides acephate and profenofos by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay with the mosquito Culex quinquefasciatus taken as experimental model.Second instar larvae were treated with LC20 of each pesticide for 24 h and induced mutations in the ...
Bhinder, Preety, Chaudhry, Asha
openaire   +3 more sources

Discrimination of Kratom Products by an Improved PCR-RFLP Method

YAKUGAKU ZASSHI, 2020
In Japan, mitragynine, 7-hydroxymitragynine and Mitragyna speciosa Korth. (M. speciosa, "Kratom") were controlled as Designated Substances under the Pharmaceutical and Medical Device Act from March 2016. In this study, the origins of 16 Kratom products obtained from the illegal drug market in Japan were investigated by DNA analyses and LC-MS analyses ...
Jun, Ogata, Maiko, Kawamura, Takashi, Hakamatsuka, Ruri, Kikura-Hanajiri   +3 more
openaire   +3 more sources

SPG4 and Dementia: Expanding the Clinical Spectrum

Annals of Clinical and Translational Neurology, Volume 13, Issue 10, Page 2002-2016, October 2026.
ABSTRACT Objective Hereditary spastic paraplegia (HSP) is a group of disorders characterized by progressive spasticity and lower limb weakness, with mutations in SPG4/SPAST being the most common cause. Detailed studies and clinical and molecular comparisons across different populations are missing.
Emanuele Panza, Arun Meyyazhagan, Eliseo Picchi, Gustavo Ribas, Ingrid Faber, Ryosuke Miyamoto, Preethi Basavaraju, Paolo Eusebi, Haripriya Kuchi Bhotla, Mario Stasi, Fabrizio Gaudiello, Francesco Patti, Filippo Maria Santorelli, Marcondes Cavalcante França Jr, José Luiz Pedroso, Orlando Graziani Povoas Barsottini, Hélio Afonso Ghizoni Teive, Peter Henry St George‐Hyslop, Toshitaka Kawarai, Antonio Orlacchio   +19 more
wiley   +1 more source

Molecular Characterization of Environmental Non-Tuberculous Mycobacteria Using PCR- RFLP Analysis of 441 Bp Heat Shock Protein 65 Fragments [PDF]

Iranian Journal of Public Health, 2012
Background: Non- Tuberculous Mycobacteria are environmental opportunistic pathogens that can be found in various terrestrial and aquatic habitats. There are an epidemiological links between species isolated in tap water and those isolated from patients ...
B Nasr-Esfahani, E Sarikhani, S Moghim, J Faghri, H Fazeli, N Hoseini, H Rezaei-Yazdi   +6 more
doaj   +1 more source

Brains for dementia research at 18: Commitment to impact the value of human post‐mortem tissue

Alzheimer's &Dementia, Volume 22, Issue 10, October 2026.
Abstract The Brains for Dementia Research (BDR) project was established to provide high quality, neuropathologically characterized post‐mortem brain tissue from a cohort of participants longitudinally assessed for outcomes that are relevant for research in relation to dementia.
Keeley J. Brookes, Nicola Barnett, Johanna S. Jackson, Emma J Kidd, Debra J. Lett, Jillian Madine, Ben Middlehurst, Laura E. Palmer, Laura Parkkinen, Andrew C. Robinson, Rebecca Sims, Lindsey Sinclair, Alexandra Stolzing, Philip Tinkler, Claire Troakes, Lauren Walker, The BDR Collaborative, Alan Thomas, Patrick G. Kehoe   +18 more
wiley   +1 more source