Results 71 to 80 of about 655,509 (235)

Molecular Characterization of Environmental Non-Tuberculous Mycobacteria Using PCR- RFLP Analysis of 441 Bp Heat Shock Protein 65 Fragments [PDF]

open access: yesIranian Journal of Public Health, 2012
Background: Non- Tuberculous Mycobacteria are environmental opportunistic pathogens that can be found in various terrestrial and aquatic habitats. There are an epidemiological links between species isolated in tap water and those isolated from patients ...
H Rezaei-Yazdi   +6 more
doaj   +2 more sources

Blood Meal Identification in Field-Captured Sand flies: Comparison of PCR-RFLP and ELISA Assays

open access: yesJournal of Arthropod-Borne Diseases, 2009
Background: We aimed to develop a PCR-RFLP assay based on available sequences of putative vertebrate hosts to iden­tify blood meals ingested by field female sand fly in the northwest of Iran.
N Maleki-Ravasan   +5 more
doaj  

Genotoxicity evaluation of acephate and profenofos by the PCR-RFLP assay

open access: yesToxicology International, 2014
In this study we have evaluated the genotoxic potential of pesticides acephate and profenofos by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay with the mosquito Culex quinquefasciatus taken as experimental model.Second instar larvae were treated with LC20 of each pesticide for 24 h and induced mutations in the ...
Bhinder, Preety, Chaudhry, Asha
openaire   +3 more sources

Polymorphism of the Gonadotropin‐Releasing Hormone Receptor Gene and Its Association With Production Traits in Isfahani Native Hens

open access: yesVeterinary Medicine and Science, Volume 12, Issue 4, July 2026.
This research was conducted to identify genetic polymorphisms of the GNRHR gene on Isfahan native hen traits and their relationship with production traits. For this purpose, blood was taken, DNA was extracted, the target fragment was amplified and directly sequenced, and finally it was blasted with the reference gene in NCBI.
Farshid Kheiri   +4 more
wiley   +1 more source

Cucumber green mottle mosaic virus: A persistent seed‐borne threat to cucurbits

open access: yesAnnals of Applied Biology, Volume 189, Issue 1, July 2026.
Cucumber green mottle mosaic virus (CGMMV) is a highly stable, seed‐borne tobamovirus that threatens global cucurbit production. Its efficient mechanical and seed transmission, long‐term environmental persistence, and the spread of Clade II CGMMV isolates drive its rapid dissemination and cause severe yield losses.
Esperanza Gea‐Caballero   +1 more
wiley   +1 more source

Differentiation of Brucella abortus and B. melitensis biovars using PCR-RFLP and REP-PCR

open access: yesNew Microbes and New Infections, 2019
Brucellosis is one of the most common zoonotic diseases of animal and human beings. This study aimed to differentiate the Brucella spp. and determines the patterns of biovars by using repetitive element palindromic (REP)-PCR and PCR restriction fragment ...
M. Amoupour   +6 more
doaj   +1 more source

Fecal DNA Identifies a Disjunct Population of an Endemic Deer (Mazama jucunda) From the Atlantic Forest, Brazil

open access: yesAnimal Genetics, Volume 57, Issue 3, June 2026.
ABSTRACT Limited knowledge of brocket deer distribution persists due to their elusive behavior and the morphological similarities among species. A population of red brocket deer (genus Mazama) was recently discovered in the Rio Doce State Park (PERD), a protected area within the Brazilian Atlantic Forest; however, species‐level identification was ...
Jeferson L. S. Freitas   +4 more
wiley   +1 more source

Primers and PCR programs for HVEM PCR-RFLP genotyping.

open access: yes, 2013
Primers and PCR programs for HVEM PCR-RFLP genotyping.
Dalin Li (91843)   +7 more
core   +1 more source

A family screening of CD19 gene mutation by PCR-RFLP

open access: yes, 2022
Introduction and aim. Mutation(s) in the gene encoding the CD19 molecule affect CD19 protein expression and primary immunodeficiency (PID) occurs. The PCR-RFLP method, which is faster and cheaper than other mutation detection methods, is rarely used in ...
Karaselek, Mehmet Ali   +13 more
core   +1 more source

Application of polymerase chain reaction (PCR) and PCR based restriction fragment length polymorphism for detection and identification of dermatophytes from dermatological specimens

open access: yesIndian Journal of Dermatology, 2008
Objective: To develop and optimize polymerase chain reaction-based restriction fragment length polymorphism (PCR-RFLP) targeting 18S rDNA and internal transcribed spacer (ITS) region of fungi for rapid detection and identification of dermatophytes ...
Bagyalakshmi R   +4 more
doaj  

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