ATF4‐mediated stress response as a therapeutic vulnerability in chordoma
Molecular Oncology, EarlyView.We screened 5 chordoma cell lines against 100+ inhibitors of epigenetic and metabolic pathways and kinases and identified halofuginone, a tRNA synthetase inhibitor. Mechanistically halofuginone induces an integrated stress response, with eIF2alpha phosphorylation, activation of ATF4 and its target genes CHOP, ASNS, INHBE leading to cell death ...
Lucia Cottone +11 more
wiley +1 more source
BMC Global and Public HealthBackground The Philippines’ high tuberculosis (TB) burden calls for effective point-of-care screening. Systematic TB case finding using chest X-ray (CXR) with computer-aided detection powered by deep learning-based artificial intelligence (AI-CAD ...
Nichel Marquez +6 more
doaj +1 more source
Scientific Reports, 2018 Single cell genome analysis methods are powerful tools to define features of single cells and to identify differences between them. Since the DNA amount of a single cell is very limited, cellular DNA usually needs to be amplified by whole-genome ...
Christiane Bäumer +4 more
doaj +1 more source
PCR-RFLP Using BseDI Enzyme for Pork Authentication in Sausage and Nugget Products [PDF]
, 2011 A polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) using BseDI restriction enzyme had been applied for identifying the presence of pork in processed meat (beef sausage and chicken nugget) including before and after frying ...
Abidin, M. Z. (M) +3 more
core
Cell surface interactome analysis identifies TSPAN4 as a negative regulator of PD‐L1 in melanoma
Molecular Oncology, EarlyView.Using cell surface proximity biotinylation, we identified tetraspanin TSPAN4 within the PD‐L1 interactome of melanoma cells. TSPAN4 negatively regulates PD‐L1 expression and lateral mobility by limiting its interaction with CMTM6 and promoting PD‐L1 degradation.
Guus A. Franken +7 more
wiley +1 more source
Comparison of PCR and microscopy for detection of Cryptosporidium parvum in human fecal specimens: clinical trial [PDF]
, 1998 PCR technology offers alternatives to conventional diagnosis of Cryptosporidium for both clinical and environmental samples. We compared microscopic examination by a conventional acid-fast staining procedure with a recently developed PCR test that can ...
Morgan, U.M. +5 more
core +1 more source
Diffusion Tensor Imaging for Assessment of Response to Neoadjuvant Chemotherapy in Patients With Breast Cancer. [PDF]
, 2016 In this study, the prognostic significance of tumor metrics derived from diffusion tensor imaging (DTI) was evaluated in patients with locally advanced breast cancer undergoing neoadjuvant therapy.
Harnish, Roy +6 more
core +1 more source
Plecstatin inhibits hepatocellular carcinoma tumorigenesis and invasion through cytolinker plectin
Molecular Oncology, EarlyView.The ruthenium‐based metallodrug plecstatin exerts its anticancer effect in hepatocellular carcinoma (HCC) primarily through selective targeting of plectin. By disrupting plectin‐mediated cytoskeletal organization, plecstatin inhibits anchorage‐dependent growth, cell polarization, and tumor cell dissemination.
Zuzana Outla +10 more
wiley +1 more source
A cardiac and subcutaneous canine dirofilariosis outbreak in a kennel in central France
Parasite, 2019 Canine dirofilarioses are nematode infections caused by two species of the genus Dirofilaria: D. immitis and D. repens. We describe here an outbreak of D. immitis and D.
Laidoudi Younes +4 more
doaj +1 more source
Analysis of the bacterial communities in the rhizosphere of Chrysanthemum via denaturing gradient gel electrophoresis of PCR- amplified 16S rRNA as well as DNA fragments coding for 16S rRNA [PDF]
, 2001 The effect of developing chrysanthemum roots on the presence and activity of bacterial populations in the rhizosphere was examined by using culture-independent methods.
Duineveld, B.M. +4 more
core +1 more source