Results 101 to 110 of about 3,342,563 (345)

A Basic Guide to Real Time PCR in Microbial Diagnostics: Definitions, Parameters, and Everything

Frontiers in Microbiology, 2017
Real time PCR (quantitative PCR, qPCR) is now a well-established method for the detection, quantification, and typing of different microbial agents in the areas of clinical and veterinary diagnostics and food safety.
P. Kralik, M. Ricchi
semanticscholar   +1 more source

Modeling hepatic fibrosis in TP53 knockout iPSC‐derived human liver organoids

Molecular Oncology, EarlyView.
This study developed iPSC‐derived human liver organoids with TP53 gene knockout to model human liver fibrosis. These organoids showed elevated myofibroblast activation, early disease markers, and advanced fibrotic hallmarks. The use of profibrotic differentiation medium further amplified the fibrotic signature seen in the organoids.
Mustafa Karabicici, Soheil Akbari, Ceyda Caliskan, Canan Celiker, Ozden Oz, Leman Binokay, Gökhan Karakulah, Serif Senturk, Esra Erdal   +8 more
wiley   +1 more source

تجزیه‌وتحلیل نواحی ژنی E1A و E1B از آدنوویروس انسانی نوع 8 در بیماران مبتلا به کراتوکونژونکتیویت آدنوویروسی در شهر اهواز [PDF]

مجله دانشگاه علوم پزشکی سبزوار
زمینه و هدف: کراتوکونژونکتیویت اپیدمیک یک اختلال عفونی حاد چشمی است که اغلب با آدنوویروس انسانی نوع D8 (HAdV-D8) مرتبط می‌باشد.  E1Aو E1B آدنوویروسی به‌ترتیب از پروتئین‌های ضروری برای شروع عفونت آدنوویروسی و اتصال به p53 سلولی می‌باشند. این مطالعه با هدف
شهرام جلیلیان, ناهید امیدی, آذرخش آذران, منوچهر مکوندی, غلامرضا خاتمی نیا, کامبیز احمدی انگالی   +5 more
doaj   +1 more source

A PCR plus restriction enzyme-based technique for detecting target-enzyme mutations at position Pro-106 in glyphosate-resistant Lolium perenne

, 2021
Hossein Ghanizadeh, Andrew G. Griffiths, Christopher E. Buddenhagen, Craig B. Anderson, K.C. Harrington   +4 more
openalex   +1 more source

PYCR1 inhibition in bone marrow stromal cells enhances bortezomib sensitivity in multiple myeloma cells by altering their metabolism

Molecular Oncology, EarlyView.
This study investigated how PYCR1 inhibition in bone marrow stromal cells (BMSCs) indirectly affects multiple myeloma (MM) cell metabolism and viability. Culturing MM cells in conditioned medium from PYCR1‐silenced BMSCs impaired oxidative phosphorylation and increased sensitivity to bortezomib.
Inge Oudaert, Lauren van den Broecke, Osman Aksoy, Judith Lind, Sonia Vallet, Arne Van der Vreken, Gamze Ates, Ann Massie, Ken Maes, Kim De Veirman, Elke De Bruyne, Karin Vanderkerken, Klaus Podar, Eline Menu   +13 more
wiley   +1 more source

Biphasic amplification of very dilute DNA samples via ‘booster‘ PCR [PDF]

, 1989
Gualberto Ruaño, Wayne A. Fenton, Kenneth K. Kídd   +2 more
openalex   +1 more source

Inhibition of CDK9 enhances AML cell death induced by combined venetoclax and azacitidine

Molecular Oncology, EarlyView.
The CDK9 inhibitor AZD4573 downregulates c‐MYC and MCL‐1 to induce death of cytarabine (AraC)‐resistant AML cells. This enhances VEN + AZA‐induced cell death significantly more than any combination of two of the three drugs in AraC‐resistant AML cells.
Shuangshuang Wu, Jianlei Zhao, Aaban Asfar Azmi, Avanti Gupte, Jenna Thibodeau, Shuang Liu, Jinli Yang, Guan Wang, Holly Edwards, Lisa A. Polin, Juiwanna Kushner, Sijana H. Dzinic, Kathryn White, Julie Boerner, Maik Hüttemann, Jay Yang, Yue Wang, Jeffrey W. Taub, Yubin Ge   +18 more
wiley   +1 more source

Mutagenic PCR. [PDF]

Genome Research, 1994
R C, Cadwell, G F, Joyce
openaire   +3 more sources

Adaptaquin is selectively toxic to glioma stem cells through disruption of iron and cholesterol metabolism

Molecular Oncology, EarlyView.
Adaptaquin selectively kills glioma stem cells while sparing differentiated brain cells. Transcriptomic and proteomic analyses show Adaptaquin disrupts iron and cholesterol homeostasis, with iron chelation amplifying cytotoxicity via cholesterol depletion, mitochondrial dysfunction, and elevated reactive oxygen species.
Adrien M. Vaquié, Davod R. Shah, Eliane E. S. Brechbühl, Michael McNicholas, Zhaoyang Xu, John H. Stockley, Laura Morcom, Diana Gold Diaz, Gemma C. Girdler, Rachel V. Seear, Gabriel Balmus, Rajiv R. Ratan, Harry Bulstrode, James A. Nathan, Manav Pathania, Kevin M. Brindle, David H. Rowitch   +16 more
wiley   +1 more source

Polymorphism of Sikumbang Jonti ducks Growth Hormone (GH) Gene using PCR-RFLP Methods

, 2023
Teguh Rafian, Yurnalis Yurnalis, Husmaini Husmaini, Firda Arlina   +3 more
openalex   +2 more sources