Results 131 to 140 of about 3,342,563 (345)

One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products.

Proceedings of the National Academy of Sciences of the United States of America, 2000
K. Datsenko, B. Wanner
semanticscholar   +1 more source

Molecular characterisation of human penile carcinoma and generation of paired epithelial primary cell lines

Molecular Oncology, EarlyView.
Generation of two normal and tumour (cancerous) paired human cell lines using an established tissue culture technique and their characterisation is described. Cell lines were characterised at cellular, protein, chromosome and gene expression levels and for HPV status.
Simon Broad, Mahmood Hachim, Tom Bertin, Karolina Penderecka, Rifat Hamoudi, Saif Khan, Rui Henrique, Natalie Bergmoser, Manit Arya, Kalle Sipila, Matteo Vietri Rudan, Asif Muneer, Aamir Ahmed   +12 more
wiley   +1 more source

Generation of linear expression constructs by one-step PCR with vaccinia DNA topoisomerase I [PDF]

, 2007
Jinhua Xiao, Xin Wen, Yongjie Liu, Robert W. Murphy, Da‐Wei Huang   +4 more
openalex   +1 more source

The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments.

Clinical Chemistry, 2009
S. Bustin, V. Beneš, J. Garson, J. Hellemans, J. Huggett, M. Kubista, Reinhold D. Mueller, T. Nolan, M. Pfaffl, G. Shipley, J. Vandesompele, C. Wittwer   +11 more
semanticscholar   +1 more source

Cis‐regulatory and long noncoding RNA alterations in breast cancer – current insights, biomarker utility, and the critical need for functional validation

Molecular Oncology, EarlyView.
The noncoding region of the genome plays a key role in regulating gene expression, and mutations within these regions are capable of altering it. Researchers have identified multiple functional noncoding mutations associated with increased cancer risk in the genome of breast cancer patients.
Arnau Cuy Saqués, Aracele Martinez‐Mendez, John Crown, Alex Eustace   +3 more
wiley   +1 more source

Analyses of PCR products using DNA templates containing a consecutive deoxyinosine sequence [PDF]

, 2004
Atsushi Kobayashi
openalex   +1 more source

Detection of circulating tumor DNA in colorectal cancer patients using a methylation‐specific droplet digital PCR multiplex

Molecular Oncology, EarlyView.
We developed a cost‐effective methylation‐specific droplet digital PCR multiplex assay containing tissue‐conserved and tumor‐specific methylation markers. The assay can detect circulating tumor DNA with high accuracy in patients with localized and metastatic colorectal cancer.
Luisa Matos do Canto, Louise Raunkilde, Jan Lindebjerg, Mads Malik Aagaard, Christina Therkildsen, Jakob Kleif, Lars Henrik Jensen, Torben Frøstrup Hansen, Rikke Fredslund Andersen   +8 more
wiley   +1 more source

Evaluation of Nested PCR in Blood, Urine and Stool in Detection of Typhoid Fever by Sequences in the VI Region of the Flagellin Gene [PDF]

, 2020
Aalaa Abouelnour, Maysaa El Sayed Zaki, Sherif M. H. Elkannishy   +2 more
openalex   +1 more source

Perspectives in educating molecular pathologists on liquid biopsy: Toward integrative, equitable, and decentralized precision oncology

Molecular Oncology, EarlyView.
Liquid biopsy enables minimally invasive, real‐time molecular profiling through analysis of circulating biomarkers in biological fluids. This Perspective highlights the importance of training pathologists through integrative educational programs, such as the European Masters in Molecular Pathology, to ensure effective and equitable implementation of ...
Marius Ilié, Umberto Malapelle, Catherine Alix‐Panabières, Claus Lindbjerg Andersen, Sandra Chlebowski, Vivien Lake, Caroline Lacoux, Virginie Lespinet‐Fabre, Olivier Bordone, Simon Heeke, Christophe Bontoux, Ellen Heitzer, Klaus Pantel, Paul Hofman   +13 more
wiley   +1 more source

Methylation-specific PCR: a novel PCR assay for methylation status of CpG islands.

Proceedings of the National Academy of Sciences of the United States of America, 1996
J. Herman, J. Graff, S. Myöhänen, B. Nelkin, S. Baylin   +4 more
semanticscholar   +1 more source