Results 241 to 250 of about 3,585,945 (273)

The impact of the PCR plateau phase on quantitative PCR

Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression, 1994
The quantitative use of the polymerase chain reaction (PCR) is often compromised by the variability of the amplification. The most useful system for quantitation by PCR involves the use of controls which are almost identical to the target and which can be amplified using the same primers as the sequences of interest.
Frank Gannon, Ciaran G. Morrison
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Colony PCR

2017
Escherichia coli and Saccharomyces cerevisiae are currently the two most important organisms in synthetic biology. E.coli is almost always used for fundamental DNA manipulation while yeast is the simplest host system for studying eukaryotic gene expression and performing large scale DNA assembly.
Azevedo, Flávio, Pereira, Humberto, Johansson, Björn   +2 more
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PCR, Real-Time PCR, Digital PCR, and Isothermal Amplification

2015
The field of molecular biology was revolutionized with the development of the polymerase chain reaction (PCR). This chapter defines PCR, reverse transcription PCR (RT-PCR), real-time PCR, digital PCR and isothermal amplification. Within each subject a brief overview of the process is given along with the required reagents or components and highlighted ...
Janine R. Hutchison, Rachel A. Bartholomew, Douglas R. Call, Timothy M. Straub   +3 more
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Direct PCR of Intact Bacteria (Colony PCR)

Current Protocols in Microbiology, 2016
AbstractThis protocol describes an efficient method for screening intact bacteria for the presence of desired DNA sequences using the polymerase chain reaction (PCR). This method is commonly referred to as colony PCR. © 2016 by John Wiley & Sons, Inc.
Brian Stevenson, William K. Arnold, Christina R. Savage, Michael E. Woodman, Michael E. Woodman   +4 more
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PCR und Real-Time PCR

2010
Die vielfaltigen Anwendungsmoglichkeiten der Polymerasekettenreaktion (polymerase chain reaction, PCR) machen sie zu einer der wichtigsten und am haufigsten eingesetzten Methoden in der molekularbiologischen Forschung und Diagnostik. Fur diese Technologie wurde der Erfinder der Methode, Kary Mullis, 1993 mit dem Nobelpreis ausgezeichnet.
Regina Konrad, Ulrich Busch
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Reverse-Transcription PCR (RT-PCR)

2013
RT-PCR is commonly used to test for genetic diseases and to characterize gene expression in various tissue types, cell types, and over developmental time courses. This serves as a form of expression profiling, but typically as a candidate approach. RT-PCR is also commonly used to clone cDNAs for further use with other molecular biology techniques (e.g.,
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Quantitative PCR

cclm, 2000
Abstract The classic molecular biology methods like Northern or Southern blot analyse non-amplified DNA or RNA, but need large amounts of nucleic acids, in many instances from tissues or cells that are heterogeneous. In contrast, polymerase chain reaction (PCR)-based techniques allow us to obtain genetic information through the specific ...
R, Jung, K, Soondrum, M, Neumaier
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Enzymatic Amplification of RNA by PCR (RT‐PCR)

Current Protocols in Molecular Biology, 2001
AbstractMethods for enzymatic amplification of RNA by the polymerase chain reaction (RT‐PCR) are highlighted in this unit. The is especially useful for rare RNAs because all steps (annealing, reverse transcription, and amplification) are performed under optimal conditions, thereby maximizing efficiency and recovery.
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Overview of PCR

Current Protocols Essential Laboratory Techniques, 2008
AbstractThe polymerase chain reaction (PCR) has revolutionized molecular biology and has enabled many other technologies and applications commonly used in research, and is part of many life science products. This unit will cover the basic principles of PCR, including how to carry out PCR in a laboratory that has not used this powerful technique ...
Christine D. Kuslich, Carl T. Yamashiro, Buena Chui   +2 more
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CODEHOP PCR and CODEHOP PCR Primer Design

2010
While PCR primer design for the amplification of known sequences is usually quite straightforward, the design, and successful application of primers aimed at the detection of as yet unknown genes is often not. The search for genes that are presumed to be distantly related to a known gene sequence, such as homologous genes in different species, paralogs
Timothy M. Rose, Timothy M. Rose, Dina Kovarik, Richard D. Boyce, Jeannette P. Staheli   +4 more
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