Results 241 to 250 of about 3,284,156 (346)

Quantification of mRNA using real-time reverse transcription PCR (RT-PCR): trends and problems.

Journal of Molecular Endocrinology, 2002
S. Bustin
semanticscholar   +1 more source

Lançamento do Pcr Creader123 OBD2 Automatic Diagnostic Tool Atualização gratuita X431 PCR123 ABS/SRS/câmbio/motor Pcr Creader 123 Hp Creader VII

, 1987
Henrique Rattner
openalex  

Transcriptional regulation of neuropeptide receptors underlies context‐dependent adaptation in Drosophila melanogaster

FEBS Open Bio, EarlyView.
Under environmental changes, the expression level of neuropeptide (NP) and neuropeptide receptor (NPR) genes changes to confer context‐dependent adaptation to the model organism Drosophila melanogaster. Through finding more regulatory elements in the NPR genes in comparison with their ligands (NPs), we found that NPR‐biased transcriptional regulation ...
SeungHeui Ryu, Yanan Wei, Zekun Wu, Tianmu Zhang, DoHoon Lee, Hadi Najafi, Woo Jae Kim   +6 more
wiley   +1 more source

A chicken lncRNA is identified as a critical regulator that increases influenza virus replication by impairing innate antiviral responses. [PDF]

Vet Res
Sajjad N, Maarouf M, Wang Y, Shrestha P, Rai KR, Chi X, Wang S, Chen JL.   +7 more
europepmc   +1 more source

Universal and rapid salt-extraction of high quality genomic DNA for PCR-based techniques.

Nucleic Acids Research, 1997
S. Aljanabi, Iciar Martinez
semanticscholar   +1 more source

Structural and functional characterization of chitinase from carnivorous plant Drosera adelae

FEBS Open Bio, EarlyView.
A class I chitinase from the carnivorous plant Drosera adelae was expressed and purified using a yeast system, revealing high enzymatic activity. Structural analyses of the catalytic and chitin‐binding domains identified key tyrosine residues involved in substrate binding, offering insights into the enzyme's adaptation for insect digestion.
Kazunari Yoneda, Yuki Naruse, Yusaku Suizu, Tomohiro Araki, Yoshikazu Hoshi, Haruhiko Sakuraba, Junji Hayashi, Toshihisa Ohshima   +7 more
wiley   +1 more source

Insights from 17 years of culture and PCR detection of animal mollicutes in a Canadian provincial laboratory. [PDF]

J Vet Diagn Invest
Ledger L, Munevar F, Nelson-Smikle P, Kellendonk C, You Q, Parker L, McRaild P, McDowall R, Eidt J, Benoit N, Bell-Rogers P, Maxie G, Cai HY.   +12 more
europepmc   +1 more source

Berberine–cinnamic acid co‐crystal effect in ameliorating hyperlipidemia might be regulated through the PI3K/AKT/mTOR/SREBP‐1 signaling pathway

FEBS Open Bio, EarlyView.
Berberine–cinnamic acid co‐crystal (BBR‐CA) inhibits the phosphorylation of the phosphatidylinositol 3‐kinase (PI3K)/AKT/mammalian target of rapamycin (mTOR) pathway, suppressing the transfer of pre‐sterol regulatory element‐binding proteins‐1 (SREBP‐1) from the endoplasmic reticulum to the nucleus. This results in a decrease in the expression level of
Wenheng Gao, Yunlong Li, Lihua Chen, Wenshuo Yang, Yong He, Ye Yang, Dengke Yin, Song Tan   +7 more
wiley   +1 more source

Rapid and Cost-Effective Digital Quantification of RNA Editing and Maturation in Organelle Transcripts by Oxford Nanopore Target-Indexed-PCR (TIP) Sequencing. [PDF]

Plant Direct
Hua Z.
europepmc   +1 more source

Establishment and optimization of the two‐step induction system for generating primordial germ cell‐like cells from chicken embryonic stem cells

FEBS Open Bio, EarlyView.
This study optimizes a two‐step induction system to generate PGCLCs from chicken ESCs. The induced cells express germline markers and migrate to gonads in ovo, offering a novel approach for avian genetic resource conservation. Primordial germ cells (PGCs) are the progenitor cells of sperm and eggs.
Zeyu Li, XianShuai Xu, GuangZheng Liu, XiaoQian Lv, JiuZhou Song, HongYan Sun, YingJie Niu, QiSheng Zuo, Wei Han, BiChun Li, Kai Jin   +10 more
wiley   +1 more source