Results 241 to 250 of about 3,745,037 (345)

Construction of hyperthermostable d‐allulose 3‐epimerase from Arthrobacter globiformis M30 using the sequence information from Arthrobacter psychrolactophilus

open access: yesFEBS Open Bio, EarlyView.
d‐Allulose can be produced from d‐fructose by d‐allulose 3‐epimerase. Based on sequence homology information, we successfully engineered thermostable mutants with the protein engineering method. By integrating positive mutations, we constructed an enzyme that exhibits hyperthermostability without a loss in the activity.
Kensaku Shimada   +3 more
wiley   +1 more source

Pathologic complete response after neoadjuvant therapy for locally advanced rectal cancer in a real-world setting: a population-based study. [PDF]

open access: yesFront Oncol
Cadili L   +8 more
europepmc   +1 more source

Maximizing sensitivity and specificity of PCR by preamplification heating

open access: green, 1991
Richard T. D’Aquila   +5 more
openalex   +2 more sources

Improved yields of long PCR products using gene 32 protein [PDF]

open access: green, 1990
Klaus Schwarz   +2 more
openalex   +1 more source

Current trends in single‐cell RNA sequencing applications in diabetes mellitus

open access: yesFEBS Open Bio, EarlyView.
Single‐cell RNA sequencing is a powerful approach to decipher the cellular and molecular landscape at a single‐cell resolution. The rapid development of this technology has led to a wide range of applications, including the detection of cellular and molecular mechanisms and the identification and introduction of novel potential diagnostic and ...
Seyed Sajjad Zadian   +6 more
wiley   +1 more source

Immune Subtyping Identifies Patients With Hormone Receptor-Positive Early-Stage Breast Cancer Who Respond to Neoadjuvant Immunotherapy (IO): Results From Five IO Arms of the I-SPY2 Trial. [PDF]

open access: yesJCO Precis Oncol
Wolf DM   +35 more
europepmc   +1 more source

Biophysical characterization and ion transport with cell‐based and proteoliposome reconstitution assays of invertebrate K+‐Cl− cotransporters

open access: yesFEBS Open Bio, EarlyView.
The K+‐Cl− cotransporters (KCCs) facilitate the symport of ions across the plasma membrane. They participate in physiological processes including neuronal regulation. Here, we characterized KCCs from Drosophila and Hydra vulgaris. Comparative analyses of transporters provide insights into the mechanism of KCC ion transport, regulation, and evolution ...
Satoshi Fudo   +4 more
wiley   +1 more source

The impact of PCR duplication on RNAseq data generated using NovaSeq 6000, NovaSeq X, AVITI, and G4 sequencers. [PDF]

open access: yesGenome Biol
Zajac N   +10 more
europepmc   +1 more source

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