Results 21 to 30 of about 3,284,156 (346)

Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes

Genome Biology, 2002
BackgroundGene-expression analysis is increasingly important in biological research, with real-time reverse transcription PCR (RT-PCR) becoming the method of choice for high-throughput and accurate expression profiling of selected genes.
J. Vandesompele, K. De Preter, F. Pattyn, B. Poppe, N. Van Roy, A. De Paepe, F. Speleman   +6 more
semanticscholar   +1 more source

Influence of Copper Oxide Nanoparticles on Gene Expression of Birch Clones In Vitro under Stress Caused by Phytopathogens

Nanomaterials, 2022
Recently, metal oxide nanoparticles (NPs) have attracted attention as promising components for the protection and stimulation of plant microclones in tissue culture in vitro.
Tatiana A. Grodetskaya, Peter M. Evlakov, Olga A. Fedorova, Vyacheslav I. Mikhin, Olga V. Zakharova, Evgeny A. Kolesnikov, Nadezhda A. Evtushenko, Alexander A. Gusev   +7 more
doaj   +1 more source

Chest CT for Typical 2019-nCoV Pneumonia: Relationship to Negative RT-PCR Testing

Radiology, 2020
Some patients with positive chest CT findings may present with negative results of real time reverse-transcription–polymerase chain- reaction (RT-PCR) for 2019 novel coronavirus (2019-nCoV). In this report, we present chest CT findings from five patients
Xingzhi Xie, Zheng Zhong, Wei Zhao, Chao Zheng, Fei Wang, Jun Liu   +5 more
semanticscholar   +1 more source

Genetic epidemiology using whole genome sequencing and haplotype networks revealed the linkage of SARS-CoV-2 infection in nosocomial outbreak

Infection Prevention in Practice, 2021
Summary: Background: A characteristic feature of SARS-CoV-2 is its ability to transmit from pre- or asymptomatic patients, complicating the tracing of infection pathways and causing outbreaks.
Fumihiro Ishikawa, Yuko Udaka, Hideto Oyamada, Keiko Ishino, Issei Tokimatsu, Hironori Sagara, Yuji Kiuchi   +6 more
doaj   +1 more source

Modulation of miR-145-5p and miR-146b-5p levels is linked to reduced parasite load in H9C2 Trypanosoma cruzi infected cardiomyoblasts

Scientific Reports, 2022
In the heart tissue of acutely Trypanosoma cruzi-infected mice miR-145-5p and miR-146b-5p are, respectively, downregulated and upregulated. Here, we used the H9C2 rat cardiomyoblast cell line infected with the Colombian T. cruzi strain to investigate the
Priscila Silva Grijó Farani, Beatriz Iandra Silva Ferreira, Daniel Gibaldi, Joseli Lannes-Vieira, Otacilio Cruz Moreira   +4 more
doaj   +1 more source

Monitoring for freshwater mussel presence in rivers using environmental DNA

Environmental DNA, 2021
In 2013, the U.S. Environmental Protection Agency (USEPA) updated its national recommended water quality criteria for the protection of aquatic life from the toxic effects of ammonia in freshwaters.
Ellen P. Preece, Michael Bryan, Samantha M. Mapes, Cara Wademan, Robert Dorazio   +4 more
doaj   +1 more source

Normalization of Real-Time Quantitative Reverse Transcription-PCR Data: A Model-Based Variance Estimation Approach to Identify Genes Suited for Normalization, Applied to Bladder and Colon Cancer Data Sets

Cancer Research, 2004
Accurate normalization is an absolute prerequisite for correct measurement of gene expression. For quantitative real-time reverse transcription-PCR (RT-PCR), the most commonly used normalization strategy involves standardization to a single ...
C. Andersen, J. L. Jensen, T. Ørntoft
semanticscholar   +1 more source

Investigating the mechanism of action of hemofugal and blood-stasis-expelling soup in the treatment of coronary heart disease based on network pharmacology [PDF]

BIO Web of Conferences
Objective: To investigate the mechanism of action of Xuefu yuyuxu Tang in the treatment of Coronary Heart Disease (CHD) based on network-based pharmacology.
·yusuf Arslan, Ma Yun, Wang Qi
doaj   +1 more source

Establishment of a murine model of congenital toxoplasmosis and validation of a qPCR assay to assess the parasite load in maternal and fetal tissues

Frontiers in Microbiology, 2023
Toxoplasma gondii is the causative agent of toxoplasmosis, a disease that affects warm-blooded animals and one third of the human population worldwide.
Jéssica S. Souza, Priscila S. G. Farani, Priscila S. G. Farani, Beatriz I. S. Ferreira, Helene S. Barbosa, Rubem F. S. Menna-Barreto, Otacilio C. Moreira, Rafael M. Mariante   +7 more
doaj   +1 more source

Chelex 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material.

BioTechniques, 2013
Procedures utilizing Chelex 100 chelating resin have been developed for extracting DNA from forensic-type samples for use with the PCR. The procedures are simple, rapid, involve no organic solvents and do not require multiple tube transfers for most ...
P. Walsh, D. A. Metzger, Mohammed Taha
semanticscholar   +1 more source