Results 81 to 90 of about 4,982,154 (391)

Real time quantitative PCR.

open access: yesGenome Research, 1996
We have developed a novel "real time" quantitative PCR method. The method measures PCR product accumulation through a dual-labeled fluorogenic probe (i.e., TaqMan Probe).
Christian A. Heid   +3 more
semanticscholar   +1 more source

Targeting EZH2 reverses thyroid cell dedifferentiation and enhances iodide uptake in anaplastic thyroid cancer

open access: yesFEBS Letters, EarlyView.
Anaplastic thyroid cancer (ATC) lacks iodide uptake ability due to MAPK activation increasing the expression of the histone methyltransferase EZH2, which represses thyroid differentiation genes (TDGs) such as the sodium iodide symporter (NIS). Dual inhibition of MAPK (U0126) and EZH2 (EPZ6438/Tazemetostat) reverses this mechanism, thus restoring TDG ...
Diego Claro de Mello   +6 more
wiley   +1 more source

Genotype/subgenotype distribution of hepatitis B virus among hemodialysis patients with chronical hepatitis B

open access: yesAnnals of Hepatology, 2012
Introduction. Hepatitis B virus (HBV) infection in hemodialysis (HD) patients is a major concern, but limited information exists on the HBV genotyping in patients on maintenance HD in Turkey.Aim.
Murat Sayan, M.D., Cengiz Dogan
doaj   +1 more source

Simple environments fail as illustrations of intelligence: A review of R. Pfeifer and C. Scheier [PDF]

open access: yes, 2001
The field of cognitive science has always supported a variety of modes of research, often polarised into those seeking high-level explanations of intelligence and those seeking low-level, perhaps even neuro-physiological, explanations.
Gobet, F, Lane, PCR
core  

Structural insights into lacto‐N‐biose I recognition by a family 32 carbohydrate‐binding module from Bifidobacterium bifidum

open access: yesFEBS Letters, EarlyView.
Bifidobacterium bifidum establishes symbiosis with infants by metabolizing lacto‐N‐biose I (LNB) from human milk oligosaccharides (HMOs). The extracellular multidomain enzyme LnbB drives this process, releasing LNB via its catalytic glycoside hydrolase family 20 (GH20) lacto‐N‐biosidase domain.
Xinzhe Zhang   +5 more
wiley   +1 more source

Monitoring the genetic variation of some Escherichia coli strains in wild birds and cattle

open access: yesOnderstepoort Journal of Veterinary Research, 2023
To date, there is limited data about the genetic relationship of Escherichia coli between wild birds and cattle because these birds act as silent vectors for many zoonotic bacteria.
Ghada A. Ibrahim   +6 more
doaj   +1 more source

Learning perceptual schemas to avoid the utility problem [PDF]

open access: yes, 1999
This paper describes principles for representing and organising planning knowledge in a machine learning architecture. One of the difficulties with learning about tasks requiring planning is the utility problem: as more knowledge is acquired by the ...
Cheng, PCH, Gobet, F, Lane, PCR
core  

PCR Based Genotyping of Lulu Cattle of Nepal for A1, A2 Type Beta-caseins [PDF]

open access: yes, 2020
Lulu is an indigenous breed of cattle (Bos taurus) found in high altitude regions of western Nepal. Population of Lulu cattle has been declining due to introgression with other exotic breeds to increase milk productivity.
Budhathoki, N. (Nabina)   +7 more
core  

ThermoPhyl : a software tool for selecting phylogenetically optimized conventional and quantitative-PCR taxon-targeted assays for use with complex samples [PDF]

open access: yes, 2011
The ability to specifically and sensitively target genotypes of interest is critical for the success of many PCR-based analyses of environmental or clinical samples that contain multiple templates.Next-generation sequence data clearly show that such ...
Dowd, Scot E.   +2 more
core   +1 more source

Interplay between circadian and other transcription factors—Implications for cycling transcriptome reprogramming

open access: yesFEBS Letters, EarlyView.
This perspective highlights emerging insights into how the circadian transcription factor CLOCK:BMAL1 regulates chromatin architecture, cooperates with other transcription factors, and coordinates enhancer dynamics. We propose an updated framework for how circadian transcription factors operate within dynamic and multifactorial chromatin landscapes ...
Xinyu Y. Nie, Jerome S. Menet
wiley   +1 more source

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