Results 91 to 100 of about 1,720,158 (289)

Identifying and characterising a plant GH1 β‐glucosidase that exhibits hydrolytic activity on N‐linked glucopyranoside

open access: yesFEBS Open Bio, EarlyView.
We report the first β‐glucosidase with demonstrated hydrolytic activity on an N‐linked glycopyranoside. The enzyme, native to maize, was biochemically characterised for this novel reaction, and structural modelling of the enzyme–substrate complex revealed several clues to the underlying reduced catalytic rate relative to its native O‐glycopyranoside ...
Hani Gharabli   +3 more
wiley   +1 more source

Setting staffing requirements for time-dependent queueing networks: the case of accident and emergency departments [PDF]

open access: yes, 2012
An incentive scheme aimed at reducing patients’ waiting times in accident and emergency departments was introduced by the UK government in 2000. It requires 98% of patients to be discharged, transferred, or admitted to inpatient care within 4 hours of ...
Dave Worthington   +6 more
core   +1 more source

Solution to uncertainty of renewable energy sources and peak hour demand in smart grid system

open access: yesMeasurement: Sensors
Today's need for environmental sustainability prompted the smart grid to incorporate more renewable energy sources. Reducing the operational costs of power generation and emission dispatch can both be accomplished with the integration of renewable energy
Govind Rai Goyal, Shelly Vadhera
doaj   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

open access: yesFEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh   +2 more
wiley   +1 more source

Avidin is evolutionarily conserved in fish but dispensable for development and resistance against Streptococcus agalactiae in zebrafish

open access: yesFEBS Open Bio, EarlyView.
The presence of biotin‐binding avidin proteins in fish and their biological significance are poorly characterized. We cataloged fish avidins and demonstrate that they are widely present and evolutionarily conserved. We created avd knockout zebrafish and show that zebavidin is dispensable for development and that resistance of avd knockout embryos in ...
Anni K. Saralahti   +5 more
wiley   +1 more source

Identifications peak hours on intersections set in Bielsko-Biała city

open access: yesScientific Journal of Silesian University of Technology. Series Transport, 2016
Traffic flow in cities is usually examined locally. This method is not effective for through traffic analysis. The paper discusses the problem of determining peak traffic hours taking into account vehicle distributions. Peak hours represent time periods of traffic flow which demand special treatment by traffic control systems.
openaire   +2 more sources

Identification and characterisation of calcitonin receptor isoforms expressed in glioblastoma derived glioma stem and U‐87 MG cells

open access: yesFEBS Open Bio, EarlyView.
Glioblastoma cells express calcitonin receptor variants (CT receptor isoforms) that may help them survive stress. Using qPCR, transcript‐specific long‐read nanopore sequencing, immunofluorescence co‐localisation and comparative sequence analysis, this study identifies a novel alternatively spliced CALCR transcript that encodes the CTb receptor isoform ...
Pragya Gupta   +7 more
wiley   +1 more source

Escape from the peak procedure [PDF]

open access: yes, 2014
The peak procedure is a timing procedure used to measure the ability of animals to time intervals. The peak procedure consists of fixed interval (FI) trials and some non-reinforced or extinction trials (EXT).
Juekarun, Phloiuma
core  

Cell surface CD11c as a neutrophil aging marker molecule

open access: yesFEBS Open Bio, EarlyView.
Cell surface CD11chi neutrophils were more aged and had better phagocytic function than CD11c−/lo neutrophils. Transcriptomic analysis of CD11chi neutrophils and CD11c−/lo neutrophils in pediatric population showed that the most difference was seen in infants.
Sophia Koutsogiannaki   +5 more
wiley   +1 more source

Static optimization of a semiflexible on‐demand public bus line for peak hours

open access: yesInternational Transactions in Operational Research
AbstractIn this paper, we study a public bus line operating between a terminal and a city center for which there exists a clear demand imbalance between the two directions during peak hours. Despite the demand imbalance, traditional public bus systems typically operate with a fixed‐route and fixed‐timetable scheme in both directions.
Dilay Aktas   +2 more
openaire   +5 more sources

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