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Detectionof pectinesterase in polyacrylamide gels
2015A rapid and sensitive method of detecting pectinesterase activity following electrophoresis or isoelectric focusing in polyacrylamide gels is described. The method uses ruthenium red and requires no addition of substrate when making the gels, thus obviating direct enzyme-subtrate contact during electrophoresis.
Alonso, Jesús, Rodríguez, M. Teresa
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On the occurrence of multiple molecular forms of pectinesterase
Archives of Biochemistry and Biophysics, 1963Abstract Evidence is presented based on differential extraction procedures, pH-dependence studies, activation or inactivation by sodium dodecyl sulfate, and differential temperature inactivation that the pulp of the banana, Musa sapientum , contains at least three molecular forms of pectinesterase.
H.O. Hultin, A.S. Levine
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Pectinesterase in the cucumber
Archives of Biochemistry and Biophysics, 1951T A, BELL, J L, ETCHELLS, I D, JONES
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Pectinesterases from Phytophthora infestans
1988Publisher Summary This chapter discusses the assay method and purification of pectinesterases from Phytophthora infestans. The chapter uses simple and less time-consuming method that has been used successfully. Like the titration method it is based on the decrease in pH of the reaction mixture due to increase in free carboxyl groups during enzyme ...
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Characterization of tomato pectinesterase.
Archives of biochemistry, 2008H R, PITHAWALA +2 more
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