Results 121 to 130 of about 2,670 (168)
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A novel form of pectinesterase in tomato
Phytochemistry, 1994The pectinesterases in tomato pericarp were fractionated by cation exchange into four forms (A-D). Form A was the most abundant and C the second most abundant. Forms A-C were further purified by gel filtration, and antibodies were raised against A and C.
A G, Warrilow, R J, Turner, M G, Jones
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Seed Science Research, 1998
AbstractMethanol accumulates in axis tissues of maturing soybean seeds, correlating with preharvest seed deterioration. Accumulation of methanol appears to be associated with the enzymic demethylation of pectin methyl esters by pectinesterase (PE; EC 3.1.1.11).
Oskar Markovič, Ralph L. Obendorf
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AbstractMethanol accumulates in axis tissues of maturing soybean seeds, correlating with preharvest seed deterioration. Accumulation of methanol appears to be associated with the enzymic demethylation of pectin methyl esters by pectinesterase (PE; EC 3.1.1.11).
Oskar Markovič, Ralph L. Obendorf
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Kinetics of papaya pectinesterase
Food Chemistry, 1995Abstract Purified papaya (Carica papaya L. var. exotica) pectinesterase (EC 3.1.1.11) was investigated for its activity as a function of NaCl, pH and temperature, and determination of its kinetic parameters. The activity was linear up to 20 min with an enzyme concentration of up to 6.14 μg.
Fayyaz, A. +4 more
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Papaya Pectinesterase inhibition by Sucrose
Journal of Food Science, 1965SUMMARY Pectinesterase (PE) was inhibited by sucrose at the same concentrations that delayed gelation of papaya purée. The inhibitory effect was linear with sucrose concentration throughout the range investigated (up to 50% sucrose).
L. W. S. CHANG +2 more
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Stability of pectinesterases of Marsh white grapefruit pulp [PDF]
Comparaison des 2 formes de pectinesterases du pomelo : forme thermostable (TS) et thermolabile (TL). La TS est aussi plus resistante au pH acide et a la proteolyse, a une plus longue duree de vie dans le jus de fruit, et est plus stable a la congelation-decongel ation.
Seymour, T.A. +5 more
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Properties of Immobilized Pectinesterase on Nylon
Journal of Macromolecular Science, Part A, 1995Abstract Pectinesterase (PE) was immobilized by covalent attachment on nylon-polyethylenimine co-polymer and the properties of the immobilized enzyme was investigated. The nylon support was activated both by using dimethyl sulphate (DMS) and triethyloxonium tetrafluoroborate (TOTFB).
Selma Ates, Sule Pekyardimci
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Action of iodine on the tomato pectinesterase
Experientia, 1977The organophosphates and carbamates did not inhibit the isolated tomato pectinesterase. Therefore this enzyme cannot be defined as serine-type esterase. The enzyme is inhibited by iodine and the inhibition (irreversible and non-competitive) is dependent on the degree of enzyme purification.
O. Markoviĉ, J. Patoĉka
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Analytical Biochemistry, 1978
Abstract A rapid assay for inhibition of pectinesterase has been developed to facilitate large scale screening for possible inhibitors. The assay gives quantitative results which can be confirmed by other assay techniques. The assay uses an agar plate in which pectin and an indicator have been incorporated.
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Abstract A rapid assay for inhibition of pectinesterase has been developed to facilitate large scale screening for possible inhibitors. The assay gives quantitative results which can be confirmed by other assay techniques. The assay uses an agar plate in which pectin and an indicator have been incorporated.
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Stability studies of papaya pectinesterase
Food Chemistry, 1995Abstract Stability studies were carried out on the purified papaya ( Carica papaya L. var. exotica ) pectinesterase (EC 3.1.1.11). The enzyme preparation was stable at 4°C in 0·02m sodium phosphate buffer (pH 7·5) solution containing 0·2m NaCl and 0·02% sodium azide and the loss in activity was less than 5% after storage for 1 year.
A Fayyaz +4 more
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Pectinesterase extraction from papaya
Food Chemistry, 1993Pectinesterase (EC.3.1.1.11) was extracted from papaya fruit (Carica papaya L.) var. Exotica. Incubation time, pH and NaCl concentration influenced the extraction process of pectinesterase from papaya fruit. A maximum activity of 6·98 units/min ml was obtained with 2 m NaCl solution, pH 8, and at 5 h incubation time.
Fayyaz, A. +4 more
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