Artificial Extracellular Matrix Proteins Containing Phenylalanine Analogues Biosynthesized in Bacteria Using T7 Expression System and the PEGylation [PDF]
, 2011 In vivo incorporation of phenylalanine (Phe) analogues into an artificial extracellular matrix protein (aECM-CS5-ELF) was accomplished using a bacterial expression host that harbors the mutant phenylalanyl-tRNA synthetase (PheRS) with an enlarged binding
Furukawa, Yuya +6 more
core +2 more sources
Cell Transplantation, 2008 The necessity to transplant islet tissue without the need for immunosuppressant therapy has led to the development of materials for immune modulation. Pegylation makes islets antigenically silent, protecting them from the adsorption of foreign protein ...
Yu-Mee Wee +12 more
doaj +1 more source
The Effect of Length and Structure of Attached Polyethylene Glycol Chain on Hydrodynamic Radius, and Separation of PEGylated Human Serum Albumin by Chromatography [PDF]
Advanced Pharmaceutical Bulletin, 2021 Purpose: This study focuses on the effect of length and structure of attached polyethylene glycol (PEG) chain on hydrodynamic radius (Rh ) and chromatographic retention of PEGylated protein. To this aim human serum albumin (HSA) as a standard protein was
Parvin Akbarzadehlaleh +3 more
doaj +1 more source
An antibody that prevents serpin polymerisation acts by inducing a novel allosteric behavior [PDF]
, 2016 Serpins are important regulators of proteolytic pathways with an antiprotease activity that involves a conformational transition from a metastable to a hyperstable state. Certain mutations permit the transition to occur in the absence of a protease; when
Faull, Sarah V. +14 more
core +1 more source
Theoretical study on pegylation reaction mechanisms of IFN-α-2a, IFN-α-2b and IFN-β-1a [PDF]
Journal of the Serbian Chemical Society, 2017 Ab initio and DFT calculations have been carried out to study the reaction mechanism between interferons (IFNs) α-2a, α-2b and β-1a and polyethylene glycol (PEG) group.
Taqavian Mohammad +3 more
doaj +1 more source
Escherichia coli TatA and TatB Proteins Have N-out, C-in Topology in Intact Cells [PDF]
, 2012 The twin arginine protein transport (Tat) system translocates folded proteins across the cytoplasmic membrane of prokaryotes and the thylakoid membrane of chloroplasts.
Alami +58 more
core +3 more sources
BF2-Azadipyrromethene Fluorophores for Intraoperative Vital Structure Identification
Molecules, 2023 A series of mono- and bis-polyethylene glycol (PEG)-substituted BF2-azadipyrromethene fluorophores have been synthesized with emissions in the near-infrared region (700–800 nm) for the purpose of fluorescence guided intraoperative imaging; chiefly ureter
Cathal Caulfield +14 more
doaj +1 more source
Drug Delivery, 2018 This work aimed to achieve long-lasting delivery of radix ophiopogonis polysaccharide (ROP) by sucrose acetate isobutyrate (SAIB)-based in situ forming systems (ISFSs) alone or combined with mono-PEGylation of ROP.
LiNa Wang +5 more
doaj +1 more source
Structure-based antigenic epitope and PEGylation improve the efficacy of staphylokinase
Microbial Cell Factories, 2017 Staphylokinase (Sak) holds promise for use in thrombolytic therapy for acute myocardial infarction. However, its immunogenicity is a major disadvantage under clinical conditions.
Yanying Xu +9 more
doaj +1 more source
A Non-Covalent Antibody Complex for the Delivery of anti-cancer drugs [PDF]
, 2019 Antibody drug conjugates (ADCs), which are obtained by coupling a potent cytotoxic agent to a monoclonal antibody (mAb), are traditionally bound in a random way to lysine or cysteine residues, with the final product's heterogeneity having an important ...
Grigoletto, Antonella +5 more
core +1 more source