Results 261 to 270 of about 401,752 (293)

Synthesis of a 125I-radiolabeled penicillin for penicillin-binding proteins studies

Analytical Biochemistry, 1983
Radioiodination of penicillin X (p-hydroxybenzylpenicillin) with 125INa, using the chloramine-T method, is simple and almost quantitative. The product thus obtained can be used without further purification for the penicillin-binding proteins (PBPs) assay.
J M, Masson, R, Labia
openaire   +2 more sources

Identification of the Penicillin-Binding Active Site of Penicillin-Binding Protein 2 of Escherichia coli

The Journal of Biochemistry, 1988
We determined the active site of penicillin-binding protein (PBP) 2 of Escherichia coli. A water-soluble form of PBP 2, which was constructed by site-directed mutagenesis, was purified by affinity chromatography, labeled with dansyl-penicillin, and then digested with a combination of proteases.
A, Takasuga, H, Adachi, F, Ishino, M, Matsuhashi, T, Ohta, H, Matsuzawa   +5 more
openaire   +2 more sources

Purification of Streptococcus faecium penicillin binding protein 5, a multifunctional penicillin-binding protein.

Microbiologica, 1986
Penicillin-binding protein 5 of Streptococcus faecium has been solubilized and partially separated from other membrane proteins by covalent affinity chromatography. PBP 5 was successively purified to homogeneity by resolution on SDS-polyacrylamide gel, elution and renaturation of penicillin-binding activity.
A. Grossato, Y. R. Cheng, E. Tonin, P. Jacques, FONTANA, Roberta   +4 more
openaire   +2 more sources

Penicillin-Binding Proteins inFusobacteriumSpecies

Anaerobe, 1996
Abstract PBPs were identified in four species ofFusobacterium. Each species had a distinctive PBP pattern, although some intra-species variation was noted. Most species had five or six PBPs, ranging in molecular weight from ∼100 kDa to ∼40 kDa. The two strains ofF. nucleatumtested had characteristic “wavy” PBP patterns.F. mortiferumwas distinctive in
K. Tunér, H.M. Wexler, D. Reeves, S.M. Finegold   +3 more
openaire   +1 more source

Penicillin-binding proteins of clostridia

Current Microbiology, 1993
The penicillin-binding protein (PBP) profiles of 33Clostridium perfringens and sixClostridium species isolated from clinically significant infections were analyzed. Three new PBPs—PBPs 2B, 4B, and 5B (84, 70, and 49 kDa respectively)—and a high-molecular-weight PBP 6 (45 kDa) were demonstrated in theC. perfringens isolates. In addition to PBPs 1 and 2,
Lynda Chalkley, Ingrid Van der Westhuyzen   +1 more
openaire   +1 more source

Analysis of Amino Acid Sequences of Penicillin-Binding Proteins 1a, 2b, and 2x in Invasive Streptococcus pneumoniae Nonsusceptible to Penicillin Isolated from Children in India

Microbial Drug Resistance, 2021
Rosemol Varghese, Ayyanraj Neeravi, Balaji Veeraraghavan   +2 more
exaly  

Investigations on the role of CH…O interactions and its impact on stability and specificity of penicillin binding proteins

Computers in Biology and Medicine, 2015
P Lavanya, Anand Anbarasu
exaly  

Penicillin-binding Protein

2006
openaire   +2 more sources

Differentiation of mycoplasmatales from bacterial protoplast L-forms by assay for penicillin binding proteins

Archives of Microbiology, 1980
H H Martin, Hans-Gerd Schiefer
exaly  

β-Lactam resistance development affects binding of penicillin-binding proteins (PBPs) of Clostridium perfringens to the fluorescent penicillin, BOCILLIN FL

Anaerobe, 2020
Miseon Park, John B Sutherland, Fatemeh Rafii   +2 more
exaly