Results 131 to 140 of about 23,204,048 (210)

Structural change study of pepsin in the presence of spermidine trihydrochloride: Insights from spectroscopic to molecular dynamics methods.

Spectrochimica Acta Part A - Molecular and Biomolecular Spectroscopy, 2023
Spermidine is an aliphatic polyamine that directs a set of biological processes. This work aimed to use UV-Vis spectroscopy, fluorescence spectroscopy, thermal stability, kinetic methods, docking, and molecular dynamic simulations to examine the ...
A. Habibi, S. Farhadian, B. Shareghi, Fatemeh Hashemi-Shahraki   +3 more
semanticscholar   +1 more source

A nephelometric pepsin method

Clinica Chimica Acta, 1969
Abstract A simple and rapid method for the determination of proteolytic activity is described. The method uses the nephelometric measurement of changes in substrate concentration. It is shown to be sensitive and accurate and may be useful in clinical situations.
E L, Gerring, E A, Allen
openaire   +2 more sources

Spectroscopic and molecular docking study of three kinds of cinnamic acid interaction with pepsin.

Spectrochimica Acta Part A - Molecular and Biomolecular Spectroscopy, 2023
In this work, under simulated physiological conditions (pH = 2.2, glycine hydrochloric acid buffer solution), the interactions of cinnamic acid (CA), m-hydroxycinnamic acid (m-CA) and p-hydroxycinnamic acid (p-CA) with pepsin were studied by fluorescence
Sujuan Zhu, Ting Wang, Ying Zheng, Qiang Shi, Qian Guo, Jing Zhu, Yiyang Mao   +6 more
semanticscholar   +1 more source

Investigation of the interaction behavior between quercetin and pepsin by spectroscopy and MD simulation methods.

International Journal of Biological Macromolecules, 2022
The ability of a therapeutic compound to bind to proteins is critical for characterizing its therapeutic impacts. We have selected quercetin (Qu), a most common flavonoid found in plants and vegetables among therapeutic molecules that are known to have ...
Fatemeh Hashemi-Shahraki, B. Shareghi, S. Farhadian   +2 more
semanticscholar   +1 more source

Exploration of binding mechanism of apigenin to pepsin: Spectroscopic analysis, molecular docking, enzyme activity and antioxidant assays.

Spectrochimica Acta Part A - Molecular and Biomolecular Spectroscopy, 2022
Pepsin plays an important role in nutrient metabolism. Apigenin (AP) is a beneficial polyphenol to human health. To enhance the bioavailability of AP and elucidate the inhibitory effect of AP on pepsin, the interaction mechanism of AP with pepsin was ...
Jinying Guo, Chuanfa Gan, Bo Cheng, Bo Cui, Fankai Yi   +4 more
semanticscholar   +1 more source

A comprehensive insight into the effects of caffeic acid (CA) on pepsin: Multi-spectroscopy and MD simulations methods.

Spectrochimica Acta Part A - Molecular and Biomolecular Spectroscopy, 2022
The interaction between caffeic acid (CA) and pepsin was investigated using multi-spectroscopy approaches and molecular dynamic simulations (MDS). The effects of CA on the structure, stability, and activity of pepsin were studied.
Fatemeh Hashemi-Shahraki, B. Shareghi, S. Farhadian, E. Yadollahi   +3 more
semanticscholar   +1 more source

A reactive aspartyl residue of pepsin

Biochemical and Biophysical Research Communications, 1968
Abstract Recently it was reported ( Hamilton, Spona, and Crowell, 1967 ) that pepsin was inactivated by an equimolar amount of 1-diazo-4-phenylbutanone-2 (DPB). The results indicated that the reaction occurred at or near the active site of pepsin. In the present communication we report evidence indicating that DPB reacts with pepsin to form an ester ...
K T, Fry, O K, Kim, C F, Kettering, J, Spona, G A, Hamilton   +4 more
openaire   +2 more sources

The interaction mechanism between gold nanoparticles and proteins: Lysozyme, trypsin, pepsin, γ-globulin, and hemoglobin.

Spectrochimica Acta Part A - Molecular and Biomolecular Spectroscopy, 2022
In this study, the interaction between gold nanoparticles (AuNPs) and proteins (including lysozyme, trypsin, pepsin, γ-globulin and hemoglobin) was investigated by UV-visible absorption spectroscopy, fluorescence spectroscopy, circular dichroism (CD ...
Xiangrong Li, W. Guo, Ruonan Xu, Zhizhi Song, Tianjun Ni   +4 more
semanticscholar   +1 more source

The Lung Allograft Microbiome Associates with Pepsin, Inflammation, and Primary Graft Dysfunction.

American Journal of Respiratory and Critical Care Medicine, 2022
RATIONALE Primary graft dysfunction (PGD) is the principal cause of early morbidity and mortality after lung transplantation. The lung microbiome has been implicated in later transplantation outcomes but has not been investigated in PGD.
J. McGinniss, S. Whiteside, R. Deek, A. Simon-Soro, J. Graham-Wooten, M. Oyster, Melanie D Brown, E. Cantu, J. Diamond, Hongzhe Li, J. Christie, F. Bushman, R. Collman   +12 more
semanticscholar   +1 more source

Pepsins—A chromatographic and kinetic comparison

Analytical Biochemistry, 1969
Abstract A simplified procedure for the preparation of pepsin from porcine pepsinogen has been developed. Chromatographic and kinetic data indicate that this procedure yields a pepsin product identical with that obtained by the conventional method.
R, Trujillo, M, Schlamowitz
openaire   +2 more sources