Results 71 to 80 of about 118,125 (294)

Primer Preactivation of Peptidoglycan Polymerases [PDF]

Journal of the American Chemical Society, 2011
Peptidoglycan glycosyltransferases are highly conserved bacterial enzymes that catalyze glycan strand polymerization to build the cell wall. Because the cell wall is essential for bacterial cell survival, these glycosyltransferases are potential antibiotic targets, but a detailed understanding of their mechanisms is lacking.
Yuriy Rebets, Suzanne Walker, Tsung-Shing Andrew Wang, Yihui Wu, Tania J. Lupoli, Hirokazu Tsukamoto, Daniel Kahne, Yuto Sumida   +7 more
openaire   +3 more sources

Analysis of the regulation of undecaprenyl diphosphate dephosphorylation in Escherichia coli

FEBS Open Bio, EarlyView.
BacA, PgpB, and YbjG phosphatases are involved in undecaprenyl phosphate (C55P) synthesis in Escherichia coli. We analyzed the lipid contents and the gene expression in the gene‐disruption strains. Undecaprenyl diphosphate (C55PP) level increased in the bacA, ybjG double‐disruption strain, but C55P levels were similar in all strains.
Tomotaka Jitsukawa, Yasushi Shigeri, Shingo Fujisaki   +2 more
wiley   +1 more source

Structural basis for the hydrolytic activity of the transpeptidase-like protein DpaA to detach Braun’s lipoprotein from peptidoglycan

mBio, 2023
The peptidoglycan layer is a defining characteristic of bacterial cells, providing them with structural support and osmotic protection. In Escherichia coli, this layer is linked to the outer membrane via the abundant membrane-anchored protein Lpp, known ...
Hsiu-Jung Wang, Víctor M. Hernández-Rocamora, Chiao-I Kuo, Kan-Yen Hsieh, Szu-Hui Lee, Meng-Ru Ho, Zhijay Tu, Waldemar Vollmer, Chung-I Chang   +8 more
doaj   +1 more source

Imaging Active Infection in vivo Using D-Amino Acid Derived PET Radiotracers. [PDF]

, 2017
Occult bacterial infections represent a worldwide health problem. Differentiating active bacterial infection from sterile inflammation can be difficult using current imaging tools. Present clinically viable methodologies either detect morphologic changes
Blecha, Joseph E, Flavell, Robert R, Kwak, Tiffany, Mutch, Christopher A, Neumann, Kiel D, Ohliger, Michael A, Rosenberg, Oren S, Sriram, Renuka, VanBrocklin, Henry F, Villanueva-Meyer, Javier E, Wilson, David M   +10 more
core   +1 more source

Peptidoglycan Perception in Plants

PLOS Pathogens, 2015
Higher eukaryotes have evolved mechanisms to detect and discriminate between a wide range of commensal and infectious microorganisms. The first inducible line of defence common between plant and animal systems is microbe-associated molecular pattern (MAMP)-triggered immunity (MTI) [1,2].
openaire   +5 more sources

A PANoptosis‐Based Signature for Survival and Immune Predication in Glioblastoma Multiforme

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective PANoptosis is a concept of total cell death characterized by pyroptosis, apoptosis, and necroptosis. We aimed to explore the clinical significance of PANoptosis‐related genes (PARGs) in glioblastoma multiforme (GBM). Methods Expression profiles of GBM were downloaded from the XENA database as a training dataset to construct a ...
Jun Yang, Da Lin, Dongyuan Liu, Dongxu Zhang, Hao Wang   +4 more
wiley   +1 more source

The Peptidoglycan-Binding Protein SjcF1 Influences Septal Junction Function and Channel Formation in the Filamentous Cyanobacterium Anabaena

mBio, 2015
Filamentous, heterocyst-forming cyanobacteria exchange nutrients and regulators between cells for diazotrophic growth. Two alternative modes of exchange have been discussed involving transport either through the periplasm or through septal junctions ...
Mareike Rudolf, Nalan Tetik, Félix Ramos-León, Nadine Flinner, Giang Ngo, Mara Stevanovic, Mireia Burnat, Rafael Pernil, Enrique Flores, Enrico Schleiff   +9 more
doaj   +1 more source

A lysozyme-like protein in Brucella abortus is involved in the early stages of intracellular replication [PDF]

, 2013
Secretion of proteins in Gram-negative bacteria is a high-energy consuming process that requires the translocation across two membranes and a periplasmic space composed of a mesh like layer, the peptidoglycan.
Czibener, Cecilia, del Giudice, Mariela Giselda, Ugalde, Juan Esteban   +2 more
core   +1 more source

Binding of Bacterial Peptidoglycan to CD14 [PDF]

Journal of Biological Chemistry, 1998
The hypothesis that soluble peptidoglycan (sPGN, a macrophage-activator from Gram-positive bacteria) binds to CD14 (a lipopolysaccharide (LPS) receptor) was tested. sPGN specifically bound to CD14 in the following three assays: binding of soluble 32P-CD14 (sCD14) to agarose-immobilized sPGN, enzyme-linked immunosorbent assay, and photoaffinity cross ...
Richard I. Tapping, Roman Dziarski, Peter S. Tobias   +2 more
openaire   +3 more sources

Current and Future Cornea Chip Models for Advancing Ophthalmic Research and Therapeutics

Advanced Biology, EarlyView.
This review analyzes cornea chip technology as an innovative solution to corneal blindness and tissue scarcity. The examination encompasses recent developments in biomaterial design and fabrication methods replicating corneal architecture, highlighting applications in drug screening and disease modeling while addressing key challenges in mimicking ...
Minju Kim, Kanghoon Choi, Amy Lin, Jungkyu Kim   +3 more
wiley   +1 more source