Cleavable N‐terminal Thioredoxin fusion enabled soluble expression and purification of otherwise insoluble SARS‐CoV‐2 Nucleocapsid (N) protein. A four‐step purification strategy yielded highly homogeneous, RNA‐free N protein. Binding assays showed high RNA affinity (Kd ~ 28 nm). The study will facilitate high‐resolution structural studies of N protein,
Shweta Singh, Gagan D. Gupta
wiley +1 more source
Impact of Ablation Energy Sources on Perceived Quality of Life and Symptom in Atrial Fibrillation Patients: A Comparative Study. [PDF]
Matteucci A+10 more
europepmc +1 more source
A psychophysical approach to the evaluation of perceived focusing quality of CRT displays [PDF]
Kwang Ho Yoon+2 more
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Genetic diversity and population structure of the Taigan dog breed
The Taigan is a rare sighthound from the mountains of Kyrgyzstan. We used DNA markers to study its genetic diversity and compare it with other sighthound breeds. Our findings show that the Taigan shares close genetic ties with the Kazakh Tazy, suggesting a common ancestry shaped by nomadic traditions and regional adaptation.
Kira Bespalova+10 more
wiley +1 more source
The Effect of the Difficulties Faced by Palliative Care Nurses on Perceived Quality of Palliative Care: A Cross-Sectional Descriptive Study. [PDF]
Öner U, Cengiz Z, Erol A.
europepmc +1 more source
Content Analysis of Quality Nursing Care Perceived by Nurses
Sung Bok Kwon+7 more
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Interaction of class III cellobiose dehydrogenase with lytic polysaccharide monooxygenase
The activity of lytic polysaccharide monooxygenase (LPMO) is supported by its auxiliary enzyme cellobiose dehydrogenase (CDH). The catalytic activity of both enzymes is coupled by electron transfer and a cyclic cascade generating substrates for both enzymes – hydrogen peroxide for LPMO and oxidized and non‐oxidized cellobiose and cello‐oligosaccharide ...
Angela Giorgianni+4 more
wiley +1 more source
Impact of the hierarchical medical system on the perceived quality of primary care in China: a quasi-experimental study. [PDF]
Zhao Y+7 more
europepmc +1 more source
Traumatic Stress, Perceived Global Stress, and Life Events: Prospectively Predicting Quality of Life in Breast Cancer Patients. [PDF]
Deanna M. Golden‐Kreutz+7 more
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Functional parameters of spermatozoa obtained by a new selection device
This study compares density gradient centrifugation and the LensHooke CA0™ device for sperm separation. DGC requires multiple steps, specialized equipment, and technical expertise. In contrast, the CA0 device offers key practical and methodological advantages, resulting in a faster, simpler, and more affordable sperm selection method.
Julio C. Chávez+8 more
wiley +1 more source