Results 261 to 270 of about 279,150 (304)
Some of the next articles are maybe not open access.
Cell cracking: Permeabilizing cells to macromolecular probes
1989The ball homogenizer described here can render cells of diameter greater than approximately 10 microns permeable to macromolecular probes. This approach may be useful for studying a variety of cellular processes which require structural integrity for function, such as secretion.
openaire +2 more sources
Chemical Permeabilization of Cells for Intracellular Product Release
2020This chapter reviews the concept of chemical permeabilization of microbial cells as it applies to the recovery of intracellular products. The topic is considered in light of the disadvantages of mechanical disruption. The chapter introduces other uses of chemical permeabilization and permeabilization of higher eucaryotes.
T J, Naglak, D J, Hettwer, H Y, Wang
openaire +2 more sources
Nuclear import of DNA in digitonin-permeabilized cells
Journal of Cell Science, 1997ABSTRACT DNA can enter intact mammalian nuclei with varying degrees of efficiency in both transfected and microinjected cells, yet very little is known about the mechanism by which it crosses the nuclear membrane. Nucleocytoplasmic transport of fluorescently labeled DNA was studied using a digitonin-permeabilized cell system.
J E, Hagstrom +5 more
openaire +2 more sources
Shock wave-induced permeabilization of mammalian cells
Physics of Life Reviews, 2018Controlled permeabilization of mammalian cell membranes is fundamental to develop gene and cell therapies based on macromolecular cargo delivery, a process that emerged against an increasing number of health afflictions, including genetic disorders, cancer and infections.
Luz M. López-Marín +3 more
openaire +2 more sources
Ribonucleotides are not channeled into DNA in permeabilized mammalian cells
Biochemical and Biophysical Research Communications, 1983Reddy and Pardee earlier reported that permeabilized CHEF/18 cells (J. Biol. Chem 257, 12526) or soluble extracts from such cells (Cell 32, 443) under the proper conditions preferentially incorporated a labeled ribonucleotide (CDP) into DNA without dilution by an excess of non-labeled dCTP, suggesting channeling of ribonucleotides into DNA by a ...
G, Spyrou, P, Reichard
openaire +2 more sources
Organellar DNA synthesis in permeabilized soybean cells
Plant Molecular Biology, 1986Cultured cells of Glycine max (L.) Merr. v. Corsoy were permeabilized by treatment with L-α-lysophosphatidylcholine (LPC). The permeabilized cells were capable of uptake and incorporation of deoxynucleoside triphosphates into DNA. Incorporation of exogenous nucleotides into DNA was linear for at least 90 minutes and the initial rate of incorporation ...
G C, Cannon, S, Heinhorst, A, Weissbach
openaire +2 more sources
[58] Permeabilized and immobilized cells
1988Publisher Summary Cells can be permeabilized without lysis of cells or destruction of the whole inner organization. Permeabilized cells are useful for the analysis of complicated metabolic processes such as DNA synthesis. After permeabilization with organic solvents cells usually are no longer viable.
openaire +1 more source
Nuclear Protein Transport in Digitonin Permeabilized Cells
2016The high concentration of cholesterol in the plasma membrane relative to the endomembranes of eukaryotic cells allows the selective permeabilization of the plasma membrane with the glycoside digitonin leaving the intracellular membrane bound organelles intact.
openaire +2 more sources