Hsc70 staining of digitonin permeabilized cells.
, 2012 Hsc70 staining of digitonin permeabilized cells. MEF, TT MEF, MEF-H2 & TT MEF-H2 heated at 44°C 20 min and harvested at specified time points after heating.
Rohan Steel (109615) +3 more
core +1 more source
Sequestration of Muscarinic Cholinergic Receptors in Permeabilized Neuroblastoma Cells [PDF]
, 1994 The feasibility of using a permeabilized preparation of human SH-SY-5Y neuroblastoma cells for studies of muscarinic acetylcholine receptor (mAChR) sequestration has been evaluated. Exposure of cells permeabilized with digitonin, streptolysin-O, or the Α-
Diana M. Slowiejko +5 more
core +1 more source
MITF maintains genome stability in nonmelanocyte lineages
Molecular Oncology, EarlyView.MITF is essential for melanocyte survival and acts as an oncogene in 10%–20% of melanomas. We show that MITF depletion causes genome instability in nonmelanocytic cells, leading to LATS2‐mediated P53 activation, cell cycle arrest, and apoptosis. This study highlights the role of MITF as a genome maintenance factor beyond the melanocyte lineage. Created
Drifa H. Gudmundsdottir +13 more
wiley +1 more source
Identification of α2 AR and REEP expression in permeabilized and non-permeabilized cells.
, 2013 HEK293A cells were transfected with HA-α2A AR, HA-α2C AR, Flag-REEP1, Flag-REEP2, or Flag–REEP6 cDNA. Forty-eight hrs post-transfections, cells were fixed with 4% PFA, under permeabilized and non-permeabilized conditions, and examined by wide field ...
Carl M. Hurt (465560) +3 more
core +1 more source
ATP-dependent regulation of phospholipase C in permeabilized 3T3 cells [PDF]
, 1990 Regulation of phospholipase C (PLC) coupled with a G-protein was studied with Swiss 3T3 cells permeabilized by digitonin. In permeabilized cells, activation ofphospholipase C required millimolar concentrations of ATP in addition to a G-protein activator,
Higashi, Kyoichiro +3 more
core +1 more source
Oncogenic DMTF1β promotes cancer cell motility by regulating autophagy through ULK1 stabilization
Molecular Oncology, EarlyView.In the current study, we demonstrate that the oncogene DMTF1β regulates ULK1 stability by reducing its proteasomal degradation in cancer cells. This stabilization enables ULK1 to induce autophagy, which in turn facilitates cancer cell migration. Consequently, reduced DMTF1β levels lead to decreased autophagy and impaired cancer cell migration.
Jun Xu +13 more
wiley +1 more source
Biochemistry and Biophysics Reports, 2017 Membrane permeant acetaldehyde and menadione induced the synergistic reduction of toluylene blue (TB) acting as non-membrane permeant redox indicator in yeast cell suspension.
Shiro Yamashoji
doaj +1 more source
Nuclear entry of hepatitis B virus capsids involves disintegration to protein dimers followed by nuclear reassociation to capsids. [PDF]
PLoS Pathogens, 2009 Assembly and disassembly of viral capsids are essential steps in the viral life cycle. Studies on their kinetics are mostly performed in vitro, allowing application of biochemical, biophysical and visualizing techniques.
Birgit Rabe +8 more
doaj +1 more source
Hsp70.1 staining of digitonin permeabilized cells.
, 2012 Hsp70.1 staining of digitonin permeabilized cells. MEF, TT MEF, MEF-H2 & TT MEF-H2 heated at 44°C 20 min and harvested at specified time points after heating.
Rohan Steel (109615) +3 more
core +1 more source
Guanine nucleotide activation of adenylate cyclase in saponin permeabilized glioma cells [PDF]
, 1986 We have compared the regulation of adenylate cyclase activity in membrane fractions from C6 glioma cells and in monolayer cultures of C6 cells that had been permeabilized with saponin.
Mark M. Rasenick +3 more
core +1 more source