Results 71 to 80 of about 279,150 (304)

Extract of mouse embryonic stem cells induces the expression of pluripotency genes in human adipose tissue-derived stem cells [PDF]

Iranian Journal of Basic Medical Sciences, 2017
Objective(s): In some previous studies, the extract of embryonic carcinoma cells (ECCs) and embryonic stem cells (ESCs) have been used to reprogram somatic cells to more dedifferentiated state. The aim of this study was to investigate the effect of mouse
Paria Motamen Salehi, Tahereh Foroutan, Arash Javeri, Masoumeh Fakhr Taha   +3 more
doaj   +1 more source

KDM7A and KDM1A inhibition suppresses tumour promoting pathways in prostate cancer

Molecular Oncology, EarlyView.
Treatment resistance is a major challenge for patients with advanced prostate cancer. This study examined an alternative approach to target the major prostate cancer‐promoting pathway by targeting epigenetic factors, whose levels are higher in tumours.
Jennie N Jeyapalan, Veronika M Metzler, Simone de Brot, Corinne L Woodcock, Anna E Harris, Jennifer Lothion‐Roy, Emeli M Nilsson, Atara Ntekim, Michael S Toss, Jenny L Persson, Francesca Khani, Brian D Robinson, Lorraine J Gudas, Emad Rakha, David M Heery, Catrin S Rutland, Nigel P Mongan   +16 more
wiley   +1 more source

Hsp70.1 staining of digitonin permeabilized cells.

, 2012
Hsp70.1 staining of digitonin permeabilized cells. MEF, TT MEF, MEF-H2 & TT MEF-H2 heated at 44°C 20 min and harvested at specified time points after heating.
Rohan Steel (109615), Robin L. Anderson (109618), Sarah L. Ellis (109617), Ryan S. Cross (109616)   +3 more
core   +1 more source

Image analysis for FLAG-SG1 transfectants demonstrates that FLAG staining is above background in both permeabilized and non-permeabilized cells.

, 2021
Amoebae were stably transfected with a plasmid for expression of full-length FLAG-tagged SG1. Immunofluorescence was used to determine the localization of SG1 in heterogeneous transfectants and imaging flow cytometry was used for analysis.
Katherine S. Ralston (11768120), Charles G. Barbieri (11768114), Tammie S. Y. Tam (11768117), Wesley Huang (6373970), Rene L. Suleiman (11768108), Samuel S. Hunter (10099278), Hannah W. Miller (3716773), Akhila Bettadapur (11768105), Maura C. Ruyechan (11768111), Matthew L. Settles (9019334)   +9 more
core   +1 more source

Flow Enabled Target Capture Halbach‐based magnetic enrichment increases circulating tumor cell capture from blood in metastatic cancer patients

Molecular Oncology, EarlyView.
Pair‐wise comparison of the CellSearch and FETCH enrichment technologies for circulating tumor cells (CTCs) from metastatic breast, prostate, and small cell lung cancer patients shows an increased capture of CTCs using FETCH enrichment. The clinical implementation of circulating tumor cells (CTCs) as a predictive tool for therapy efficacy in the ...
Michiel Stevens, Anouk Mentink, Tom Niessink, Frank Coumans, Leonie Mekenkamp, Ruchi Bansal, Jeroen Hiltermann   +6 more
wiley   +1 more source

Glycolytic flux in permeabilized freshly isolated vascular smooth muscle cells

, 1998
To determine whether channeling of glycolytic intermediates can occur in vascular smooth muscle (VSM), we permeabilized freshly isolated VSM cells from hog carotid arteries with dextran sulfate.
Christopher D. Hardin, Dorian R. Finder
core   +1 more source

Exploring the functional domain and the target of the tetanus toxin light chain in neurohypophysial terminals [PDF]

, 1994
The tetanus toxin light chain blocks calcium induced vasopressin release from neurohypophysial nerve terminals. Here we show that histidine residue 233 within the putative zinc binding motif of the tetanus toxin light chain is essential for the ...
Weller, U., Dayanithi, G., Yamasaki, S., Niemann, H., Gratzl, Manfred, Stecher, B., Höhne-Zell, B., Binz, T.   +7 more
core   +1 more source

Nuclear entry of hepatitis B virus capsids involves disintegration to protein dimers followed by nuclear reassociation to capsids. [PDF]

PLoS Pathogens, 2009
Assembly and disassembly of viral capsids are essential steps in the viral life cycle. Studies on their kinetics are mostly performed in vitro, allowing application of biochemical, biophysical and visualizing techniques.
Birgit Rabe, Mildred Delaleau, Andreas Bischof, Michael Foss, Irina Sominskaya, Paul Pumpens, Christian Cazenave, Michel Castroviejo, Michael Kann   +8 more
doaj   +1 more source

USP29‐regulated noncanonical stabilization of the hypoxia‐inducible factor‐α in aggressive prostate cancer

Molecular Oncology, EarlyView.
We identify USP29 as the only DUB mirroring CA9 expression, a marker of hypoxia and HIF pathway activation associated with PCA aggressiveness. USP29 stabilizes HIF‐1α and HIF‐2α via a noncanonical mechanism that is independent of PHD/pVHL activity yet relies on proteasomal regulation, establishing USP29 as a previously unrecognized regulator of hypoxic
Amelie S Schober, Leire Moreno‐Cugnon, Laura Martínez‐Pérez, Amaia Ercilla, Amaia Zabala‐Letona, Adrián Vicente‐Barrueco, Maider Fagoaga‐Eugui, Saioa Garcia‐Longarte, Blanca Calle‐Ciborro, Encarnación Pérez‐Andrés, Onintza Carlevaris, Sara Pozo, Isabel Mendizabal, Ugo Mayor, Arkaitz Carracedo, Violaine Sée, Edurne Berra   +16 more
wiley   +1 more source

How DASPMI reveals mitochondrial membrane potential : fluorescence decay kinetics and steady-state anisotropy in living cells

, 2008
Spectroscopic responses of the potentiometric probe 2-(4-(dimethylamino)styryl)-1-methylpyridinium iodide (DASPMI) were investigated in living cells by means of a time- and space-correlated single photon counting technique.
Ramadass, Radhan, Bereiter-Hahn, Jürgen
core   +1 more source