Results 1 to 10 of about 256 (152)

Membrane Disintegration Caused by the Steroid Saponin Digitonin Is Related to the Presence of Cholesterol

open access: yesMolecules, 2015
In the present investigation we studied the molecular mechanisms of the monodesmosidic saponin digitonin on natural and artificial membranes. We measured the hemolytic activity of digitonin on red blood cells (RBCs). Also different lipid membrane models (
Ana J García-Sáez   +2 more
exaly   +3 more sources

Downregulation of ATP1A1 Expression by Panax notoginseng (Burk.) F.H. Chen Saponins: A Potential Mechanism of Antitumor Effects in HepG2 Cells and In Vivo

open access: yesFrontiers in Pharmacology, 2021
The Na+/K+-ATPase α1 subunit (ATP1A1) is a potential target for hepatic carcinoma (HCC) treatment, which plays a key role in Na+/K+ exchange, metabolism, signal transduction, etc.
Xiao-Yi Feng   +5 more
doaj   +1 more source

Experimental substantiation of permeabilized hepatocytes model for investigation of mitochondria in situ respiration [PDF]

open access: yesThe Ukrainian Biochemical Journal, 2015
To verify experimentally the model of permeabilized hepatocytes, the degree of cell permeability was assessed using trypan blue and polarographycally determined cell respiration rate upon succinate (0.35 mM) and α-ketoglutarate (1 mM) oxidation ...
V. M. Merlavsky   +3 more
doaj   +1 more source

Isolation of Mycoplasma Membranes by Digitonin [PDF]

open access: yesJournal of Bacteriology, 1972
The cell membrane of Mycoplasma hominis was isolated by lysing the cells with digitonin. Electron microscopy and chemical, density gradient, and electrophoretic analyses of the membrane proteins showed the membranes so obtained, like those isolated by osmotic lysis, to be relatively free of cytoplasmic contaminants.
S, Rottem, S, Razin
openaire   +2 more sources

In Vitro Effect of Cholesterol and Different Sugars on Digitonin Production in Multiplied Shoots of Digitalis purpurea L. Plant

open access: yesIraqi Journal of Pharmaceutical Sciences, 2017
Production of  the steroidal saponin digitonin  in multiplied shoots of Digitalis purpurea , (var. Excelsior Mixed) has been achieved in vitro by two experiments. In the experiment 1, shoot tips ( 1cm length ) explants  from the sterilized seedlings were
Zainab J.Awad
doaj   +3 more sources

Inactivation and activation of various membranal enzymes of the cholesterol biosynthetic pathway by digitonin.

open access: yesJournal of Lipid Research, 1989
The activity of rat liver microsomal squalene epoxidase is inhibited effectively by digitonin. Concentrations of 0.8 to 1.2 mg/ml of digitonin cause total inhibition of microsomal (0.75 mg protein/ml) squalene epoxidase either in microsomes that were ...
H Eilenberg   +3 more
doaj   +1 more source

Analysis of Mitochondrial Calcium Retention Capacity in Cultured Cells: Permeabilized Cells Versus Isolated Mitochondria

open access: yesFrontiers in Physiology, 2021
In response to various pathological stimuli, such as oxidative and energy stress accompanied by high Ca2+, mitochondria undergo permeability transition (PT) leading to the opening of the non-selective PT pores (PTP) in the inner mitochondrial membrane ...
Sehwan Jang   +3 more
doaj   +1 more source

Identification and Genetic Characterization of Mycoplasma Species Affecting Respiratory System in Egyptian Cattle [PDF]

open access: yesBenha Veterinary Medical Journal, 2021
: Mycoplasma species are the main important causative agents causing pneumonia in cattle, there are about 200 species or more. The current work aimed to investigate various mycoplasma spp.
Ashraf Abd El-Tawab   +3 more
doaj   +1 more source

Isolation and identification of phospholipids of bovine rhodopsin

open access: yesJournal of Lipid Research, 1969
Phospholipids present in digitonin solutions of bovine rhodopsin have been identified and assayed. Digitonin interferes with extraction of lipids by the usual methods; digitonin was therefore removed from the preparation as an ergosterol digitonide ...
Ralph G. Adams
doaj   +1 more source

The effect of digitonin on egg quality, cholesterol content in eggs, biochemical and haematological parameters in laying hens

open access: yesCzech Journal of Animal Science, 2004
The aim of the present study was to describe the effect of digitonin on egg quality, cholesterol content in eggs, some parameters of serum lipid metabolism and blood picture of laying hens. The experiment was split into 3 groups (8 laying hens per group);
E. Tůmová   +3 more
doaj   +1 more source

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