Results 21 to 30 of about 26,012 (183)
Plasma Membrane and Chromaffin Granule Characteristics in Digitonin-Treated Chromaffin Cells [PDF]
, 1985 Digitonin permeabilizes the plasma membranes of bovine chromaffin cells to Ca 2+ , ATP, and proteins and allows micromolar Ca 2+ in the medium to stimulate directly catecholamine secretion.
Ronald W. Holz +3 more
core +1 more source
Separation of the Inner and Outer Mitochondrial Membrane in HeLa Cells
Bio-Protocol, 2014 Mitochondria are organelles that have important functions in oxidative phosphorylation, fatty acid oxidation and apoptosis signaling. They have two distinct membranes, outer membrane (OM) and inner membrane (IM).
Naotaka Nishimura, Masato Yano
doaj +1 more source
Nuclear entry of hepatitis B virus capsids involves disintegration to protein dimers followed by nuclear reassociation to capsids. [PDF]
PLoS Pathogens, 2009 Assembly and disassembly of viral capsids are essential steps in the viral life cycle. Studies on their kinetics are mostly performed in vitro, allowing application of biochemical, biophysical and visualizing techniques.
Birgit Rabe +8 more
doaj +1 more source
Molecular Therapy: Nucleic Acids, 2018 Antisense oligonucleotide (AON) therapy for Duchenne muscular dystrophy has drawn great attention in preclinical and clinical trials, but its therapeutic applications are still limited due to inefficient delivery.
Mingxing Wang +4 more
doaj +1 more source
Synergistic and additive effects of epigallocatechin gallate and digitonin on Plasmodium sporozoite survival and motility. [PDF]
PLoS ONE, 2010 BackgroundMost medicinal plants contain a mixture of bioactive compounds, including chemicals that interact with intracellular targets and others that can act as adjuvants to facilitate absorption of polar agents across cellular membranes.
Janina K Hellmann +3 more
doaj +1 more source
Separation of Thylakoid Protein Complexes with Two-dimensional Native-PAGE
Bio-Protocol, 2018 The hierarchical composition and interactions of the labile thylakoid protein complexes can be assessed by sequential 2D-native gel-electrophoresis system.
Marjaana Rantala +2 more
doaj +1 more source
Journal of Lipid Research, 1974 Hepatic sterol synthesis is accelerated in animals after a single dose of allylisopropylacetamide, a chemical known to be porphyrogenic. Evidence is provided to show that enhanced sterol biosynthesis is due to an increased formation of mevalonic acid ...
Luigi Taddeini +2 more
doaj +1 more source
Hsc70 staining of digitonin permeabilized cells.
, 2012 Hsc70 staining of digitonin permeabilized cells. MEF, TT MEF, MEF-H2 & TT MEF-H2 heated at 44°C 20 min and harvested at specified time points after heating.
Rohan Steel (109615) +3 more
core +1 more source
Freeze-fracture identification of sterol-digitonin complexes in cell and liposome membranes. [PDF]
, 1978 To advance our understanding of the organization of cholesterol within cell membranes, we used digitonin in freeze-fracture investigations of model lipid vesicles and tissues.
P M Elias +7 more
core +1 more source
Hsp70.1 staining of digitonin permeabilized cells.
, 2012 Hsp70.1 staining of digitonin permeabilized cells. MEF, TT MEF, MEF-H2 & TT MEF-H2 heated at 44°C 20 min and harvested at specified time points after heating.
Rohan Steel (109615) +3 more
core +1 more source