Unveiling the Novel Anti - Tumor Potential of Digitonin, a Steroidal Saponin, in Gastric Cancer: A Network Pharmacology and Experimental Validation Study [PDF]
Drug Design, Development and TherapyDongdong Lu,1,2 Leijie Huang,1 Chunyan Weng2 1Department of Gastroenterology, Ningbo No. 2 Hospital, Ningbo, Zhejiang Province, 315000, People’s Republic of China; 2Department of Gastroenterology, The First Affiliated Hospital of Zhejiang Chinese Medical
Lu D, Huang L, Weng C
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Salmonella enterica serovar typhi limits the potency of typhoid toxin and ADP-ribosylating toxin AB to establish a persistent infection. [PDF]
FEBS Open BioThe two catalytic subunits of typhoid toxin dissociate from the holotoxin in the ER of an intoxicated cell, but only CdtB exits the ER to generate immunosuppressive effects. PltA is retained in the ER and sequestered from its cytosolic target, thus allowing the anti‐inflammatory effects of CdtB to promote intestinal colonization.
Zabala-Rodriguez MC +4 more
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Further Characterization of Dopamine Release by Permeabilized PC 12 Cells [PDF]
, 1987 Rat pheochromocytoma cells (PC 12) permeabilized with staphylococcal α-toxin release [3H]dopamine after addition of micromolar Ca2+. This does not require additional Mg2+-ATP (in contrast to bovine adrenal medullary chromaffin cells).
Ahnert-Hilger, G. +3 more
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On the Interaction between Digitonin and Cholesterol in Langmuir Monolayers [PDF]
, 2016 In this article, we describe the effect of a highly hemolytic saponin, digitonin, on model lipids cholesterol and dipalmitoylphosphatidylcholine (DPPC) using a combination of tensiometric (surface pressure and dilatational surface elasticity ...
Orczyk, Marta +17 more
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Pathogens, 2022 In this study, we demonstrate that Trypanosoma cruzi epimastigotes previously grown in LIT medium supplemented with 20 mM galactose and exposed to sub-lethal concentrations of hydrogen peroxide (100 μM) showed two-fold and five-fold viability when ...
Ángel Lobo-Rojas +5 more
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Flow Cytometric Analysis of the Cell Cycle in Transfected Cells Without Cell Fixation
BioTechniques, 1999 We describe a simple and rapid protocol for the flow cytometric analysis of the cell cycle in transfected cells using a green fluorescent protein anchored in the intracellular membranes of the endoplasmic reticulum (ER-GFP) as a transfection marker.
Dimitri G. Pestov +2 more
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Application of flow cytometry to determine differential redistribution of cytochrome c and Smac/DIABLO from mitochondria during cell death signaling. [PDF]
PLoS ONE, 2012 Mitochondrially mediated apoptosis is characterized by redistribution of proteins from mitochondria to cytoplasm following permeabilization of the outer mitochondrial membrane.
Heling Ng +2 more
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Journal of Lipid Research, 2012 Quantitative analysis of mitochondrial FA β-oxidation (FAO) has drawn increasing interest for defining lipid-induced metabolic dysfunctions, such as in obesity-induced insulin resistance, and evaluating pharmacologic strategies to improve β-oxidation ...
Regina Ensenauer +11 more
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Rapid quantitative procedure for removing cholesterol from butter fat
Journal of Lipid Research, 1967 Cholesterol is rapidly and quantitatively removed from butter fat by passage of the fat in hexane or benzene solution over a column of Celite impregnated with an aqueous solution of digitonin.
D.P. Schwartz +2 more
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Rapid enrichment of mitochondria from mammalian cell cultures using digitonin
MethodsX, 2021 We describe here a simple method to enrich mitochondrial fractions from mammalian cells for downstream analyses in the lab. Mitochondria purification involves cell lysis followed by separation of the organelles from the rest of the cellular components ...
Bhavna Dixit +4 more
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