Results 11 to 20 of about 256 (152)
Pathogens, 2022 In this study, we demonstrate that Trypanosoma cruzi epimastigotes previously grown in LIT medium supplemented with 20 mM galactose and exposed to sub-lethal concentrations of hydrogen peroxide (100 μM) showed two-fold and five-fold viability when ...
Ángel Lobo-Rojas +5 more
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Flow Cytometric Analysis of the Cell Cycle in Transfected Cells Without Cell Fixation
BioTechniques, 1999 We describe a simple and rapid protocol for the flow cytometric analysis of the cell cycle in transfected cells using a green fluorescent protein anchored in the intracellular membranes of the endoplasmic reticulum (ER-GFP) as a transfection marker.
Dimitri G. Pestov +2 more
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Application of flow cytometry to determine differential redistribution of cytochrome c and Smac/DIABLO from mitochondria during cell death signaling. [PDF]
PLoS ONE, 2012 Mitochondrially mediated apoptosis is characterized by redistribution of proteins from mitochondria to cytoplasm following permeabilization of the outer mitochondrial membrane.
Heling Ng +2 more
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Journal of Lipid Research, 2012 Quantitative analysis of mitochondrial FA β-oxidation (FAO) has drawn increasing interest for defining lipid-induced metabolic dysfunctions, such as in obesity-induced insulin resistance, and evaluating pharmacologic strategies to improve β-oxidation ...
Regina Ensenauer +11 more
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Rapid quantitative procedure for removing cholesterol from butter fat
Journal of Lipid Research, 1967 Cholesterol is rapidly and quantitatively removed from butter fat by passage of the fat in hexane or benzene solution over a column of Celite impregnated with an aqueous solution of digitonin.
D.P. Schwartz +2 more
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Rapid enrichment of mitochondria from mammalian cell cultures using digitonin
MethodsX, 2021 We describe here a simple method to enrich mitochondrial fractions from mammalian cells for downstream analyses in the lab. Mitochondria purification involves cell lysis followed by separation of the organelles from the rest of the cellular components ...
Bhavna Dixit +4 more
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Nuclear entry of hepatitis B virus capsids involves disintegration to protein dimers followed by nuclear reassociation to capsids. [PDF]
PLoS Pathogens, 2009 Assembly and disassembly of viral capsids are essential steps in the viral life cycle. Studies on their kinetics are mostly performed in vitro, allowing application of biochemical, biophysical and visualizing techniques.
Birgit Rabe +8 more
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Separation of the Inner and Outer Mitochondrial Membrane in HeLa Cells
Bio-Protocol, 2014 Mitochondria are organelles that have important functions in oxidative phosphorylation, fatty acid oxidation and apoptosis signaling. They have two distinct membranes, outer membrane (OM) and inner membrane (IM).
Naotaka Nishimura, Masato Yano
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Studies on the fractionation by digitonin of chloroplast membranes [PDF]
FEBS Letters, 1980 11. The basic action of digitonin was to separate the grana thyla- koids from the intergrana thylakoids [Z]. Thus the heavy fraction (10 000 X g pellet, enriched in photo- sytem II) consisted mostly of grana, while the light fraction (10 000 X g supernatant, enriched in photo- system I) consisted mostly of intergrana membranes.
Jennings R. C. +3 more
openaire +2 more sources