Results 31 to 40 of about 26,012 (183)

Complete in vitro DNA replication of SV40 chromatin in digitonin-treated permeable cells. [PDF]

open access: yes, 1980
A permeable cell system has been developed by treatment with digitonin for studying in vitro DNA replication of chromatin. DNA replication of simian virus 40 nucleoprotein complexes (SV40 chromatin) in digitonin-treated permeable cells was analyzed by ...
Oda, Takuzo   +3 more
core   +1 more source

Isolation and identification of new constituents in milk fat

open access: yesJournal of Lipid Research, 1970
After removal of cholesterol on a digitonin column, the unsaponifiable matter of milk fat was examined for alcoholic substituents. Derivatization with pyruvic acid chloride 2,6-dinitrophenylhydrazone and fractionation of the derivatives gave four main ...
C.R. Brewington   +2 more
doaj   +1 more source

Quantitative assessment of cytosolic Salmonella in epithelial cells. [PDF]

open access: yesPLoS ONE, 2014
Within mammalian cells, Salmonella enterica serovar Typhimurium (S. Typhimurium) inhabits a membrane-bound vacuole known as the Salmonella-containing vacuole (SCV). We have recently shown that wild type S.
Leigh A Knodler   +2 more
doaj   +1 more source

Hydrogen-bond assisted, aggregation-induced emission of digitonin

open access: yes, 2015
Contrary to the conventional aggregation-induced emission (AIE) luminogens obtained from the addition of poor solvents to the solution of organic molecules in good solvents, we serendipitously observed the AIE phenomenon arising from the addition of a ...
Joseph, K, Mathew, MS, Sreenivasan, K
core   +1 more source

Biosynthesis of squalene and cholesterol by cell-free extracts of adult rat brain

open access: yesJournal of Lipid Research, 1969
Cell-free extracts of adult rat brain incubated with mevalonic acid-2-14C synthesize 14C-labeled nonsaponifiable fractions consisting largely of squalene-14C.
M.T. Kelley   +3 more
doaj   +1 more source

ALG-2 participates in recovery of cells after plasma membrane damage by electroporation and digitonin treatment. [PDF]

open access: yesPLoS ONE, 2018
The calcium binding protein ALG-2 is upregulated in several types of cancerous tissues and cancer cell death may be a consequence of ALG-2 downregulation.
Jonas M la Cour   +6 more
doaj   +1 more source

Mycoplasma isolation in milk samples from dairy herds in Chile

open access: yesAustral Journal of Veterinary Sciences, 2021
Mycoplasma bovine mastitis is a highly contagious disease, usually associated with clinical cases refractory to antibiotic treatment. The aim of this study was the isolation of Mycoplasma species in cattle milk samples from dairy herds in Chile.
Fernando Ulloa   +3 more
doaj   +1 more source

Nuclear Pore Mechanotransduction in Oncology: A Structural Axis of Vulnerability for Targeted Intervention

open access: yesAdvanced Materials, EarlyView.
Cancer‐associated NPC remodeling creates a high‐flux, low‐stringency nuclear state that supports malignant adaptation but increases mechanical fragility. Targeting the FG‐barrier or NPC scaffold may drive mechanostat failure, envelope rupture, DNA damage, and loss of nuclear integrity.
Sílvio Terra Stefanello   +5 more
wiley   +1 more source

Digitonin enhances the efficacy of carboplatin in liver tumour after intra-arterial administration

open access: yes, 1997
Platinum-containing drugs enter the cell slowly and have a poor tissue penetration. Increasing the permeability of the cell membrane might increase the intracellular drug concentration.
Lindnér, Per,   +4 more
core   +1 more source

Charting Endocrine Progenitors Across Species and Organs

open access: yesAdvanced Science, EarlyView.
Endocrine progenitors give rise to the hormone‐producing cells of the pancreas and intestine. Using single‐cell multiomics and proteomics, this study compares these progenitors across species, systems, and organs, mapping the conserved and species‐specific gene regulatory networks that guide their formation.
Changying Jing   +21 more
wiley   +1 more source

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