Results 41 to 50 of about 26,012 (183)

Effect of digitonin on rat myometrium subcellular membrane fractions

, 1981
Isopycnic centrifugation experiments using sucrose density gradients showed that in digitonin-treated microsomes the distribution of the plasma membrane (PM) marker 5′-nucleotidase was shifted to higher densities.
J. Crankshaw, E. E. Daniel, C. Y. Kwan, A. K. Grover   +3 more
core   +1 more source

Cancer‐Associated BCL‐2 Mutants Reveal Mechanisms Towards Venetoclax Resistance

Advanced Science, EarlyView.
Venetoclax (VEN) resistance in chronic lymphocytic leukemia arises from diverse BCL2 mutations. We map mechanisms contributing to VEN resistance across common BCL‐2 variants. G101V and D103Y reduce drug binding and increase sequestration of pro‐apoptotic proteins. V156D blocks VEN allosterically.
Jonas Aufdermauer, Ian de Ridder, Mahjoobeh Ehsani, Marasim Khan, Justin Kale, Lukas P. Frenzel, David Andrews, Spyridoula Karamanou, Ana J. Garcia‐Saez, Geert Bultynck   +9 more
wiley   +1 more source

NSD2 Coordinates the Neurogenic‐to‐Gliogenic Transition via H3K36me2‐Dependent Activation of the EGFR‐ERK Pathway

Advanced Science, EarlyView.
NSD2 coordinates the neurogenic‐to‐gliogenic transition in the developing neocortex through H3K36me2‐dependent activation of EGFR–ERK signaling. Loss of NSD2 disrupts astroglial and oligodendroglial development, whereas ERK activation rescues gliogenic defects in vitro and in vivo.
Hanxue Chen, Mengyuan Li, Lin Hou, Bin Yin, Boqin Qiang, Ran Gao, Pengcheng Shu, Xiaozhong Peng   +7 more
wiley   +1 more source

Activation of caspase-3-like protease by digitonin-treated lysosomes [PDF]

, 1998
Apoptosis, a naturally occurring programmed cell death or cell `suicide', has been paid much attention as one of the critical mechanisms for morphogenesis and tissue remodeling.
Utsumi, Kozo, Furuno, Takashi, Kozo Utsumi, Utsumi, Toshihiko, Munehisa Yabuki, Masayasu Inoue, Inoue, Masayasu, Toshihiko Utsumi, Takashi Furuno, Ishisaka, Rumi, Tomoko Kanno, Yabuki, Munehisa, Kanno, Tomoko, Rumi Ishisaka   +13 more
core   +1 more source

Single‐Cell Profiling Reveals a Protective WNT5A‐ATF3‐FOSB Signaling Axis in Hair Follicle Stem Cells During Androgenetic Alopecia

Advanced Science, EarlyView.
Androgenetic alopecia (AGA) is a common form of hair loss with limited treatment options. Silencing of WNT5A signaling, which is widely known as the trigger of the ncWNT signaling pathway, happens in hair follicle stem cells from balding areas. It leads to downregulation of ATF3 and its target FOSB.
Ruiyu Luo, Ke Fang, Changjiang Zhao, Jiachao Xiong, Yuan Zhu, Mingyang Lu, He Yan, Zihan Li, Baojin Wu, Hua Jiang, Yufei Li   +10 more
wiley   +1 more source

Effects of digitonin on the intracellular content of rat hepatocytes: implications for its use in the study of intralobular heterogeneity.

, 1993
Anterograde or retrograde perfusion of rat liver with digitonin selectively permeabilizes the periportal or the perivenous zone of the hepatic lobule.
J Y Scoazec, A Moreau, L Racine, G Feldmann, D Bernuau   +4 more
core   +1 more source

Monitoring Nuclear Import with GFP-Variant Fusion Proteins in Digitonin-Permeabilized Cells

BioTechniques, 2001
A convenient assay for monitoring nuclear localization signal-mediated nuclear import of green fluorescent protein (GFP)-variant fusion proteins has been developed.
John R. Petrulis, Gary H. Perdew
doaj   +1 more source

Comprehensive benchmarking of CITE-seq versus DOGMA-seq single cell multimodal omics

Genome Biology, 2022
The recently developed method TEA-seq and similar DOGMA-seq single cell trimodal omics assays provide unprecedented opportunities for understanding cell biology, but independent evaluation is lacking.
Zhongli Xu, Elisa Heidrich-O’Hare, Wei Chen, Richard H. Duerr   +3 more
doaj   +1 more source

Autophagy Orchestrates Anti‐Tumor Immunity to Enhance Chemosensitivity via the FBXW2/C/EBPβ/TIMP‐2 Axis in Colorectal Cancer

Advanced Science, EarlyView.
Chemotherapy leverages tumor‐cell autophagy to reshape the colorectal cancer immune microenvironment. Autophagy degrades FBXW2 to promote C/EBPβ‐mediated TIMP‐2 transcription and MMP‐9 suppression, driving intra‐tumoral CD8+ T‐cell infiltration. Targeting MMP‐2/9 or restoring TIMP‐2 overcomes chemo‐resistance in autophagy‐deficient tumors. ABSTRACT The
Bing Cheng, Chaoying Wei, Zhitong Niu, Jinxin Chen, Guojie Long, Yingzhen Weng, Qiufeng Liu, Yue Jiang, Panpan Wang, Ronghua Zhang, Qiang Yu, Xiaojian Wu, Wenyu Wang   +12 more
wiley   +1 more source

Differential binding of proteins to peroxisomes in rat hepatoma cells: unique association of enzymes involved in isoprenoid metabolism

Journal of Lipid Research, 1999
Farnesyl diphosphate synthase (FPPS: EC2.5.1.10), a key enzyme in isoprenoid metabolic pathways, catalyzes the synthesis of farnesyl diphosphate (FPP) an intermediate in the biosynthesis of both sterol and non-sterol isoprenoid end products.
Sita D. Gupta, Ryan S. Mehan, Terese R. Tansey, Hua-Tang Chen, Gertrud Goping, Israel Goldberg, Ishaiahu Shechter   +6 more
doaj   +1 more source