Results 61 to 70 of about 26,012 (183)
Ultrastructural Localization of Digitonin-Cholesterol Complex in the Adrenal Cortex [PDF]
, 1988 The ultrastructural localization of cholesterol was studied by digitonin reaction which has been used to identify the free cholesterol for electron microscopy.
Tsuchiyama, Hideo, Kawai, Kioko
core +1 more source
Journal of Lipid Research, 1982 Subcellular localization of nonesterified cholesterol has been determined in normal human fibroblasts from cultures incubated with human low density lipoprotein (LDL).
H S Kruth +4 more
doaj +1 more source
Sources and regulation of nitric oxide synthesis in uterus smooth muscle cells [PDF]
The Ukrainian Biochemical Journal, 2019 It was proved that NO synthesis in isolated mitochondria of rat uterus smooth muscle depended on the entry of exogenous Ca2+ to mitochondria (inhibited by 1-10 mM Mg2+ in the absence of ATP and by 10 μM ruthenium red) and was suppressed by calmodulin ...
H. V. Danylovych +4 more
doaj +1 more source
Identification of Novel Interacting Proteins of FUZ and GPR161
PROTEOMICS, EarlyView.ABSTRACT Protein–protein interactions are central to the dynamic regulation of signaling pathways and provide critical insight into the cellular mechanisms underlying human disease. Our previous study demonstrated biochemical and genetic interactions between FUZ and GPR161 in sonic hedgehog signaling during spinal neural tube development. In this study,
Gabriella Salazar +3 more
wiley +1 more source
Effects of purified myosin light chain kinase on myosin light chain phosphorylation and catecholamine secretion in digitonin-permeabilized chromaffin cells [PDF]
, 1987 Many non-muscle cells including chromaffin cells contain actin and myosin. The 20,000 dalton light chain subunits of myosin can be phosphorylated by a Ca 2+ /calmodulin-dependent enzyme, myosin light chain kinase.
Lee, Sung A. +3 more
core +1 more source
In vivo studies of sterol and squalene secretion by human skin
Journal of Lipid Research, 1974 This work was aimed at studying the quantity and composition of sterols and squalene secreted by the human skin. Lipids secreted by the entire skin were recovered by Soxhlet extraction of the clothing worn by a patient for 24 hr with a chloroform ...
T. Nikkari +2 more
doaj +1 more source
Phytotherapy Research, EarlyView.Schematic diagram illustrating the underlying mechanisms by which ECM and its active components activate the FANCD2‐cGAS/STING/IFN‐I signaling axis, thus enhancing the therapeutic efficacy of anti‐PD‐1 in NSCLC. ABSTRACT Activation of cGAS/STING/IFN‐I signaling pathway serves as a potential therapeutic strategy to enhance CD8+ T cells infiltration and ...
Han‐Chen Wang +9 more
wiley +1 more source
Yeast, EarlyView.We present cryoBN‐PAGE, a method that enables blue native polyacrylamide gel electrophoresis analysis of mitochondrial respiratory complexes and supercomplexes directly from whole yeast cells using cryogenic milling. This approach eliminates the need for mitochondrial isolation, reducing time and cost while preserving native protein complexes.
Lizeth J. Camacho Lopez +3 more
wiley +1 more source
A quad‐cistronic fluorescent biosensor system for real‐time detection of subcellular Ca2+ signals
British Journal of Pharmacology, EarlyView.Background and Purpose The calcium ion (Ca2+) is a versatile cellular messenger regulating a variety of biological processes. Compounds modulating subcellular Ca2+ signals hold substantial pharmacological potential. Advances in fluorescent biosensors have revolutionised Ca2+ imaging.
Anna Lischnig +7 more
wiley +1 more source
Rescue of pATOM36‐depleted T. brucei by human MTCH1/2 reveals common features of protein insertases
The FEBS Journal, EarlyView.Human MTCH1 and MTCH2 (with an N‐terminal extension) functionally complement the loss of pATOM36 in T. brucei, indicating that these evolutionarily distant proteins recognize a common set of substrates and share fundamental principles of mitochondrial outer membrane protein insertion. Structural analyses reveal a common hydrophilic cavity, supporting a
Stephan Berger +4 more
wiley +1 more source