Results 71 to 80 of about 26,012 (183)
Detection of cholesterol by digitonin conjugated gold nanoparticles
Functionalized colloidal gold is widely used for qualitative and quantitative detection of specific analytes. We report here a novel modification of gold nanoparticles by digitonin, a glycoside used for precipitating membrane cholesterol.
core +1 more source
Permeabilization of yeast cells (Kluyveromyces fragilis) to lactose by digitonin
Kluyveromyces fragilis, the lactose-fermenting yeast, was made permeable to the disaccharide lactose using the surfactant digitonin. Optimal beta-galactosidase activity was observed when the cells were treated with 0.1% digitonin at room temp. for 30 min.
Katwa, L. C. +3 more
core +1 more source
ISOLATION OF MYCOPLASMAS FROM EQUINE IN BAGHDAD AREA
Out of 225 specimences taken from nasal, Vaginal, eye swabs, milk synovial fluid and from internal organsof horses. Twelve isolates were recovered as follows 9 isolates from nasal swabs, 3 isolates from vaginal swabs.
Abdul S. Al-Safar +1 more
doaj +1 more source
The mitochondrial membrane‐shaping machinery MICOS is assembled surprisingly early during the life cycle of Trypanosoma brucei, before the mitochondrion acquires its mature architecture or full respiratory capacity. Our study also identifies two previously uncharacterized proteins associated with MICOS, providing new insight into the early organization
Michala Boudová +5 more
wiley +1 more source
A rapid radioassay procedure for plasma lecithin-cholesterol acyltransferase.
A rapid and accurate single step procedure is described for the assay of lecithin-cholesterol acyltransferase activity. After incubation, using radiolabeled cholesterol as the substrate, an ethanolic solution of digitonin is added directly to the ...
U Piran, R J Morin
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Daily Heat Stress Induces Donor‐Side Limitation of PSI Via Downregulation of the Cyt b6f Complex
ABSTRACT The rapidly warming climate is driving increasingly frequent and intense heat waves worldwide, posing major challenges to plant metabolism and growth. Despite the central role of photosynthesis in plant growth, the effects of prolonged heat stress on the primary reactions of photosynthesis remain poorly understood.
Laura Laihonen +5 more
wiley +1 more source
The kinetics of ATP-driven Ca 2+ uptake by the dense tubules were studied in digitonin-permeablized human blood platelets. Digitonin at 3 μg/ml was shown capable of permeablizing the plasma membrane to lactate dehydrogenase and the cytoplasmic Ca 2 ...
Jy, Wenche, Haynes, Duncan H
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Ambivalence of drug metabolism: Exploration of cilazapril and candesartan prodrugs for transdermal delivery and search for toxic reactive intermediates of N(G)-acylated hetarylpropylguanidines [PDF]
Figuratively, drug metabolism can be considered as two sides of the same coin; one leading to desired, beneficial effects, e.g. by activation of prodrugs, whereas the other one results in adverse side effects due to toxic metabolites.
Ertel, Miriam
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Macrophage extracellular traps (METs) represent a recently discovered complex defence mechanism that is distinct from phagocytosis and involves the release of DNA and antibacterial proteins.
Thang Ngoc Nguyen +7 more
doaj +1 more source
Effect of cholesterol feeding and fasting on sterol synthesis in seventeen tissues of the rat
Rates of sterol synthesis were measured in 17 tissues of the rat, and the responsiveness of these rates to cholesterol feeding and to fasting was determined. The liver and gastrointestinal tract together account for 90% of synthetic activity of the whole
John M. Dietschy, Marvin D. Siperstein
doaj +1 more source

