Results 71 to 80 of about 26,012 (183)

Detection of cholesterol by digitonin conjugated gold nanoparticles

open access: yes, 2011
Functionalized colloidal gold is widely used for qualitative and quantitative detection of specific analytes. We report here a novel modification of gold nanoparticles by digitonin, a glycoside used for precipitating membrane cholesterol.

core   +1 more source

Permeabilization of yeast cells (Kluyveromyces fragilis) to lactose by digitonin

open access: yes, 1989
Kluyveromyces fragilis, the lactose-fermenting yeast, was made permeable to the disaccharide lactose using the surfactant digitonin. Optimal beta-galactosidase activity was observed when the cells were treated with 0.1% digitonin at room temp. for 30 min.
Katwa, L. C.   +3 more
core   +1 more source

ISOLATION OF MYCOPLASMAS FROM EQUINE IN BAGHDAD AREA

open access: yesThe Iraqi Journal of Veterinary Medicine, 1986
Out of 225 specimences taken from nasal, Vaginal, eye swabs, milk synovial fluid and from internal organsof horses. Twelve isolates were recovered as follows 9 isolates from nasal swabs, 3 isolates from vaginal swabs.
Abdul S. Al-Safar   +1 more
doaj   +1 more source

Trypanosomal MICOS Is Assembled on Non‐Respiring Mitochondrial Crista Precursors and Associates With Two Integral Microproteins

open access: yesMolecular Microbiology, EarlyView.
The mitochondrial membrane‐shaping machinery MICOS is assembled surprisingly early during the life cycle of Trypanosoma brucei, before the mitochondrion acquires its mature architecture or full respiratory capacity. Our study also identifies two previously uncharacterized proteins associated with MICOS, providing new insight into the early organization
Michala Boudová   +5 more
wiley   +1 more source

A rapid radioassay procedure for plasma lecithin-cholesterol acyltransferase.

open access: yesJournal of Lipid Research, 1979
A rapid and accurate single step procedure is described for the assay of lecithin-cholesterol acyltransferase activity. After incubation, using radiolabeled cholesterol as the substrate, an ethanolic solution of digitonin is added directly to the ...
U Piran, R J Morin
doaj   +1 more source

Daily Heat Stress Induces Donor‐Side Limitation of PSI Via Downregulation of the Cyt b6f Complex

open access: yesPlant, Cell &Environment, EarlyView.
ABSTRACT The rapidly warming climate is driving increasingly frequent and intense heat waves worldwide, posing major challenges to plant metabolism and growth. Despite the central role of photosynthesis in plant growth, the effects of prolonged heat stress on the primary reactions of photosynthesis remain poorly understood.
Laura Laihonen   +5 more
wiley   +1 more source

Calcium uptake and release characteristics of the dense tubules of digitonin-permeabilized human platelets

open access: yes, 1988
The kinetics of ATP-driven Ca 2+ uptake by the dense tubules were studied in digitonin-permeablized human blood platelets. Digitonin at 3 μg/ml was shown capable of permeablizing the plasma membrane to lactate dehydrogenase and the cytoplasmic Ca 2 ...
Jy, Wenche, Haynes, Duncan H
core   +1 more source

Ambivalence of drug metabolism: Exploration of cilazapril and candesartan prodrugs for transdermal delivery and search for toxic reactive intermediates of N(G)-acylated hetarylpropylguanidines [PDF]

open access: yes, 2012
Figuratively, drug metabolism can be considered as two sides of the same coin; one leading to desired, beneficial effects, e.g. by activation of prodrugs, whereas the other one results in adverse side effects due to toxic metabolites.
Ertel, Miriam
core   +1 more source

Store-operated calcium entry facilitates LPS-induced superoxide anion-dependent macrophage extracellular traps

open access: yesOpen Biology
Macrophage extracellular traps (METs) represent a recently discovered complex defence mechanism that is distinct from phagocytosis and involves the release of DNA and antibacterial proteins.
Thang Ngoc Nguyen   +7 more
doaj   +1 more source

Effect of cholesterol feeding and fasting on sterol synthesis in seventeen tissues of the rat

open access: yesJournal of Lipid Research, 1967
Rates of sterol synthesis were measured in 17 tissues of the rat, and the responsiveness of these rates to cholesterol feeding and to fasting was determined. The liver and gastrointestinal tract together account for 90% of synthetic activity of the whole
John M. Dietschy, Marvin D. Siperstein
doaj   +1 more source

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