Results 91 to 100 of about 26,012 (183)

The effect of digitonin-containing fixatives on the retention of free cholesterol and cholesterol esters

open access: yes, 1978
The influence of several fixation and dehydration procedures on the retention of free cholesterol and cholesterol esters was studied in filter paper preparations.
Gent, C.M. van   +3 more
core  

The cholesterol-complexing agent digitonin modulates ligand binding of the bovine hippocampal serotonin1A receptor [PDF]

open access: yes, 2005
The serotonin1A (5-HT1A) receptor is an important member of the superfamily of seven transmembrane domain G-protein-coupled receptors. We have examined the modulatory role of cholesterol on the ligand binding of the bovine hippocampal 5-HT1A receptor by ...
Pucadyil, Thomas J.   +2 more
core   +1 more source

Comprehensive in vitro profiling of traditional and emerging stimulants at monoamine transporters and the 5‐HT2A receptor

open access: yesBritish Journal of Pharmacology, Volume 183, Issue 19, Page 5871-5894, October 2026.
A pharmacological profiling of 58 synthetic stimulants of various classes at dopamine, norepinephrine, and serotonin transporters and the 5‐HT2A receptor, most showing distinct class‐dependent profiles, with some indicating a high abuse potential. Background and Purpose New psychoactive substances (NPSs) often emerge on the illicit drug market with ...
Darta Stalberga   +9 more
wiley   +1 more source

Determination of the subcellular localization of TcPRODH enzyme by digitonin extraction.

open access: yes, 2013
Panel (A) intact epimastigotes were treated with digitonin (0–5 mg/mL) and the pyruvate kinase (PK) (inverted triangle), hexokinase (HK) (diamond), citrate synthase (CS) (circle) and proline dehydrogenase (PRODH) (square) activities were measured.
Brian Suárez Mantilla (435600)   +8 more
core   +1 more source

Digitonin fractionation followed by Western blot analysis.

open access: yes, 2013
FV1 [HV-LmDAT NEO] and FV1 [HV-LmDAT-ΔC3 NEO] were fractionated in the presence of digitonin as described in Materials and Methods. Cell supernatants were then subjected to Western blot analysis in the presence of monoclonal anti-V5 antibodies (V5), and ...
Tongtong Zhu (339166)   +5 more
core   +1 more source

Phosphomevalonate kinase is a cytosolic protein in humans

open access: yesJournal of Lipid Research, 2004
In the past decade, a predominant peroxisomal localization has been reported for several enzymes functioning in the presqualene segment of the cholesterol/isoprenoid biosynthesis pathway.
Sietske Hogenboom   +5 more
doaj   +1 more source

THE INHIBITORY EFFECT OF DEHYDROEPIANDROSTERONE ON THE ACTIVITY AND ISOENZYME PATTERNS OF LIVER GLUCOSE 6-PHOSPHATE DEHYDROGENASE DURING DIFFERENT STAGES OF GROWTH [PDF]

open access: yesJournal of Sciences, Islamic Republic of Iran, 1990
The effect of dehydroepiandrosterone (DHEA) on the activity and isoenzyme patterns of liver glucose 6- phosphate dehydrogenase (G6PD) was investigated in rats during different stages of growth.
doaj  

Human neutrophils permeabilized with digitonin respond with lysosomal enzyme release when exposed to micromolar levels of free calcium [PDF]

open access: yes, 1986
We have recently reported that human neutrophils can be permeabilized with the cholesterol complexing agent saponin and that these cells can be induced to secrete the granule enzyme lysozyme in response to micromolar levels of free calcium. We now report
Smolen, James E.   +2 more
core   +1 more source

Stabilization of cholesterol in myelin with digitonin: observation with polarized light.

open access: yes, 1977
Cholesterol can be preserved in tissues dehyrated for light microscopy by incorporating digitonin into glutaraldehyde fixation. Fresh and fixed frozen sections of rat sciatic nerve exhibit strong, radially positive birefringence when viewed with ...
L C Saland, L M Napolitano
core   +1 more source

Cа2+ transporting systems in secretory cells of the rat exorbital lacrimal gland. І. Ca2+ pumps of plasma membrane and endoplasmic reticulum

open access: yesБіологічні студії, 2012
The mechanism of secretion by lacrimal glands, the innervation and orientation effects, and the mechanism of transduction of primary agonists are similar to the major salivary glands.
A. B. Kotliarova, V. V. Manko
doaj   +1 more source

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